A glance at isotherapy to control weed germination
Fontanieu Gwladys 1, A-C
Darmency Henri 2, A,C-D  
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Institut Technique de l’Agriculture Biologique (ITAB), Fédération Régionale des AgroBiologistes de Champagne-Ardenne, Chalons-en-Champagne, France
Institut National de la Recherche Agronomique (INRA), 21065, Dijon, France
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Darmency Henri   

Institut National de la Recherche Agronomique (INRA), 21065, Dijon, France
Online publish date: 2019-04-04
Submission date: 2018-07-02
Acceptance date: 2019-03-19
Journal of Plant Protection Research 2019;59(1):19–25
Currently more and more research is being done on integrated weed management to reduce or avoid herbicide use. Some growers are already using isotherapic dilutions to control weeds in organic farming. Isotherapy is different from homeopathy because it uses diluted and potentized (succussed) solutions of alcoholic macerate of the very pest causing health troubles. We set up a germination experiment to test if isotherapic dilutions of leaf macerate of annual ryegrass affect the dynamics of its seed germination in Petri dishes. Our results were diverse, from no effect to 10% more growing degree days necessary to reach 50% germination. It is doubtful that so low an effect will contribute to integrated weed management unless the slightly delayed germination triggers secondary effects at other life stages. This is in accordance with the scientific literature on that topic: two-fifths of the reports showed no effect, two-fifths resulted in positive responses and one-fifth had diverse responses for the criteria tested.
The authors have declared that no conflict of interests exist.
Altieri M.A., Nicholls C.I., Montalba R. 2017. Technological approaches to sustainable agriculture at a crossroads: an agroecological perspective. Sustainability 9 (3): 349. DOI: https://doi.org/10.3390/su9030....
Awan T.H., Chauhan B.S. 2016. Effect of emergence time, inter- and intra-specific competition on growth and fecundity of Echinochloa crus-galli in dry-seeded rice. Crop Protection 87: 98−107. DOI: https://doi.org/10.1016/j.crop....
Barzman M., Bàrberi P., Birch A.N.E., Boonekamp P., Dachbrodt-Saaydeh S., Graf B., Hommel B., Jensen J.E., Kiss J., Kudsk P., Lamichhane J.R., Messéan A., Moonen A.C., Ratnadass A., Ricci P., Sarah J.L., Sattin M. 2015. Eight principles of integrated pest management. Agronomy for Sustainable Development 35 (4): 1199–1215. DOI: https://doi.org/10.1007/s13593....
Baumgartner S., Thurneysen A., Heusser P. 2004. Growth stimulation of dwarf peas (Pisum sativum L.) through homeopathic potencies of plant growth substances. Forschende Komplementarmedizin Klassische Naturheilkunde 11 (5): 281−292. DOI: https://doi.org/10.1159/000082....
Baumgartner S., Shah D., Shaller J., Kämpfer U., Thurneysen A., Heusser P. 2008. Reproducibility of dwarf pea shoot growth stimulation by homeopathic potencies of gibberellic acid. Complementary Therapies in Medicine 16 (4): 183−191. DOI: https://doi.org/10.1016/j.ctim....
Berger M., Bertin T., Kleinhans S. 2011. Expérimentation d’isothérapiques en culture maraîchère. [Isotherapy experimentation in market gardens.]. Cahiers de Biotherapie 228: 64−67.
Betti L., Trebbi G., Majewsky V., Scherr C., Shah-Rossi D., Jäger T., Baumgartner S. 2009. Use of homeopathic preparations in phytopathological models and in field trials: a critical review. Homeopathy 98 (4): 244–266. DOI: https://doi.org/10.1016/j.homp....
Carneiro S.M.T.P.G., Romano E.D.B., Pignoni E., Teixeira M.Z., Vasconcelos M.E.C., Gomes J.C. 2010. Effect of biotherapic of Alternaria solani on the early blight of tomato-plant and the in vitro development of the fungus. International Journal of High Dilution Research 9 (33): 147−155.
Diniz L.P., Maffia L.A., Dhingra O.D., Casali V.W.D., Santos R.H.S., Mizubuti E.S.G. 2006. Avaliacao de produtos alternativos para contrôle da requeima do tomateiro. [Quantification of the efficacy of alternative products for tomato late blight control.]. Fitopatologia Brasileira 31 (2): 171−179.
Endler P.C., Matzer W., Reich C., Reischl T., Hartmann A.M., Thieves K., Pfleger A., Hoföcker U., Lothaller H., Scherer-Pongratz W. 2011. Seasonal variation of the effect of extremely diluted agitated gibberellic acid (10e-30) on wheat stalk growth − a multi researcher study. The Scientific World Journal 11: 1667−1678. DOI: https://doi.org/10.1100/2011/4....
Endler P.C., Scherer-Pongratz W., Lothaller H., Stephen S. 2015. Wheat and ultrahigh diluted gibberellic acid − further experiments and re-analysis of data. Homeopathy 104 (4): 257−262. DOI: https://doi.org/10.1016/j.homp....
Gama E.V.S., Silva F., Santos I., Malheiro R., Fermino Soares A.C., Pereira J.A., Armond C. 2015. Homeopathic drugs to control red rot disease in sisal plants. Agronomy for Sustainaible Development 35 (2): 649–656. DOI: https://doi.org/10.1007/s13593....
Giesel A., Boff M.I.C., Boff P. 2012. The effect of homeopathic preparations on the activity level of Acromyrmex leaf-cutting ants. Acta Scientarium, Agronomy 34 (4): 445−451. DOI: https://doi.org/10.4025/actasc....
Giesel A., Boff M.I.C., Boff P. 2017. Dynamized high dilutions for management of the leafcutter ant Acromyrmex laticeps Emery (Hymenoptera: Formicidae). Acta Scientarium, Agronomy 39 (4): 497−503. DOI: https://doi.org/10.4025/actasc....
Gundel P.E., Martinez-Ghersa M.A., Ghersa C.M. 2008. Dormancy, germination and ageing of Lolium multiflorum seeds following contrasting herbicide selection regimes. European Journal of Agronomy 28 (4): 606–613. DOI: https://doi.org/10.1016/j.eja.....
Hamman B., Koning G., Him Lok K. 2003. Homeopathically prepared gibberellic acid and barley seed germination. Homeopathy 92 (3): 140−144. DOI: https://doi.org/10.1016/S1475-....
Hartung H., Schiest S., Matzer W., Endler P. 2010. Wheat germination (20 h) and extremely diluted gibberellic acid (10e-30). Explorative experiments on a fundamental homoeopathy research model. European Journal of Integrated Medicine 2 (4): 224−245. DOI: https://doi.org/10.1016/j.euji....
Hribar-Marko S., Graunke H., Scherer-Pongratz W., Lothaller H., Endler P. 2013. Pre-stimulation of wheat seedling development with gibberellic acid (10e-3, 10e-4 and 10e-5) followed by application of an agitated high dilution of the same hormone. International Journal of High Dilution Research 12 (42): 26−39.
ISTA (International Seed Testing Association). 1996. International Rules for Seed Testing. Rules 1996. Seed Science and Technology 27 (Supplement). Zurich, ISTA, 335 pp.
Jäger T., Scherr C., Shah D., Majewsky V., Wolf U., Betti L., Baumgartner S. 2015. The use of plant-based bioassays in homeopathic basic research. Homeopathy 104 (4): 277−282. DOI: https://doi.org/10.1016/j.homp....
Jäger T., Scherr C., Shah D., Majewsky V., Betti L., Trebbi G., Bonamin L., Simões-Wüst A.P., Wolf U., Simon M., Heusser P., Baumgartner S. 2011. Use of homeopathic preparations in experimental studies with abiotically stressed plants. Homeopathy 100 (4): 275−287. DOI: https://doi.org/10.1016/j.homp....
Kiefer P., Matzer W., Schiest S., Hartung H., Schwärzler I., Seunig R., Hofäcker J., Endler P.C. 2012. Wheat germination and highly diluted agitated gibberelic acid (10−30) − a multi researcher study. International Journal of High Dilution Research 11 (39): 45−59.
Majewsky V., Arlt S., Shah D., Scherr C., Jäger T., Betti L., Trebbi G., Bonamin L., Klocke P., Baumgartner S. 2009. Use of homeopathic preparations in experimental studies with healthy plants. Homeopathy 98 (4): 228–243. DOI: https://doi.org/10.1016/j.homp....
Majewsky V., Scherr C., Arlt S.P., Kiener J., Frrokaj K., Schindler T., Klocke P., Baumgartner S. 2014. Reproducibility of effects of homeopathically potentised gibberellic acid on the growth of Lemna gibba L. in a randomized and blind bioassay. Homeopathy 103 (2): 113−126. DOI: https://doi.org/10.1016/j.homp....
Matzer W., Hartmann M., Endler P.C. 2010. Wheat growth (7 days) and extremely diluted gibberellic acid (10e-30): Repetitive experiments on a fundamental homeopathy research model. European Journal of Integrated Medicine 2 (4): 245. DOI: https://doi.org/10.1016/j.euji....
Metcalf R.L., Luckmann W.H. eds. 1982. Introduction to Integrated Pest Management. New York, Wiley, 577 pp.
Modolon T.A., Boff P., Boff M.I.C., Miquelluti D.J. 2012a. Homeopathic and high dilution preparations for pest management to tomato crop under organic production system. Horticultura Brasileira 30 (1): 51−57. DOI: https://doi.org/10.1590/S0102-....
Modolon T.A., Boff P., da Rosa J.M., de Sousa P.M.R., Miquelluti D.J. 2012b. Post-harvest quality of tomato fruits treated with high dilution preparations. Horticultura Brasileira 30 (1): 58−63. DOI: https://doi.org/10.1590/S0102-....
Moot D.J., Scott W.R., Roy A.M., Nicholls A.C. 2000. Base temperature and thermal time requirements for germination and emergence of temperate pasture species. New Zealand Journal of Agricultural Research. 43 (1): 15−25. DOI: https://doi.org/10.1080/002882....
Pfleger A., Hofäcker J., Scherer-Pongratz W., Lothaller H., Reich C., Endler P. 2011. The effect of extremely diluted agitated gibberellic acid (10e-30) on wheat seedling development − a two researcher study. Complementary Therapies in Medicine 19 (3): 164−169. DOI: https://doi.orgctim.2011.03.00....
Ruehl A.T., Donath T.W., Otte A., Eckstein R.L. 2016. Impacts of short-term germination delay on fitness of the annual weed Agrostemma githago (L.). Seed Science Research 26 (2): 93−100. DOI: https://doi.org/10.1017/S09602....
Rupp L.C.D., Boff M.I.C, Boff P., Gonçalves P.A. de S., Botton M. 2012. High dilution of Staphys agria and fruit fly biotherapic preparations to manage South American fruit fly, Anastepha fraterculus, in organic peach orchards. Biological Agriculture and Horticulture 28 (1): 41−48. DOI: https://doi.org/10.1080/014487....
Scherr C., Simon M., Spranger J., Baumgartner S. 2007. Duckweed (Lemna gibba L.) as a test organism for homeopathic potencies. Journal of Alternative and Complementary Medicine 13 (9): 931−937. DOI: https://doi.org/10.1089/acm.20....
Scherr C., Simon M., Spranger J., Baumgartner S. 2009. Effect of potentized substances on growth rate of the water plant Lemna gibba L. Complementary Therapies in Medicine 17 (2): 63−70. DOI: https://doi.org/10.1016/j.ctim....
Shah-Rossi D., Heusser P., Baumgartner S. 2009. Homeopathic treatment of Arabidopsis thaliana plants infected with Pseudomonas syringae. The Scientific World Journal 9: 320−330. DOI: https://doi.org/10.1100/tsw.20....
Silviera H.R.O., Ferraz E.O., Matos C.C., Honorio I.C., Alvarenga I.C.A., Guilherme D.O., Tuffi Santos L.D., Martins E.R., Costa C.A. 2009. Use of homoeophatic preparations in the management of nutsedge (Cyperus rotundus L.). Horticultura Brasileira 27 (2, Supplement): S2420-S2424.
Stanhill G. 1990. The comparative productivity of organic agriculture. Agriculture, Ecosystems and Environment 30 (1−2): 1−26. DOI: https://doi.org/10.1016/0167-8....
Vallée P. 2004. Etude de l’effet de champs électromagnétiques basse fréquence sur les propriétés physico-chimiques de l’eau. [Study of the effects of the action of low-frequency electromagnetic fields on physicochemical properties of water]. Thèse de doctorat, Université Pierre et Marie Curie, Paris. Available on: https://tel.archives-ouvertes.... [Accessed: July 1, 2018].
Wyss E., Tamm L., Siebenwirth J., Baumgartner S. 2010. Homeopathic preparations to control the rosy apple aphid (Dysaphis plantaginea Pass.). The Scientific World Journal 10: 38−48. DOI: https://doi.org/10.1100/tsw.20....