ORIGINAL ARTICLE
A new bacterial disease on blueberry (Vaccinium corymbosum) caused by Pseudomonas spp.
 
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Research Institute of Horticulture, Pomology Division, Pomologiczna 18, 96-100 Skierniewice, Poland
CORRESPONDING AUTHOR
Monika Kałużna
Research Institute of Horticulture, Pomology Division, Pomologiczna 18, 96-100 Skierniewice, Poland
Submission date: 2012-09-19
Acceptance date: 2012-11-11
 
Journal of Plant Protection Research 2013;53(1):32–36
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ABSTRACT
In 2011, leaf spot disease was observed on the blueberry (Vaccinium corymbosum) cv. Nelson growing on a commercial field located in Central Poland. The disease symptoms could be seen as russet brown, irregular spots. The diameter of the spots was 0.3–0.5 cm, and the spots often coalesced. From these leaf spots, a fluorescent bacterium was repeatedly isolated in almost pure culture. Polymerase chain reaction (PCR) using primers Ps-for and Ps-rev, specific for Pseudomonas spp. confirmed that they belong to this genus. Based on LOPAT tests [levan production from sucrose (L), presence of oxidase (O), pectolytic activity on potato (P), the presence of arginine dihydrolase (A), hypersensitivity reaction on tobacco (T)], 6 isolates were classified to the LOPAT group Ib – group of Pseudomonas syringae subsp. savastanoi and Pseudomonas delphini, and one isolate to group Ia – P. syringae. All isolates caused a hypersensitivity reaction on tobacco plants, and symptoms similar to those under natural conditions, when young leaves of blueberry cv. Nelson were inoculated. Sequence analysis of 16S rRNA and rpo B genes showed the highest similarity of 6 studied strains to the species P. avellanae. Further taxonomic study is necessary to enable definitive classification of these isolates. It is the first time that a bacterial disease caused by the Pseudomonas spp. was observed in Poland.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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