RAPID COMMUNICATION
Antifungal activity of Solanum extracts against phytopathogenic Curvularia lunata
| 1 | Instituto de Ciencias Biológicas, Universidad de Ciencias y Artes de Chiapas, UNICACH , Mexico |
Online publish date: 2018-10-03
Submission date: 2018-03-04
Acceptance date: 2018-08-28
Journal of Plant Protection Research 2018;58(3):311–315
KEYWORDS:
Curvularia lunatabotanical extractscontrol plant diseasesleaf spot diseasephytopathogen inhibitionplant antifungals
TOPICS:
ABSTRACT:
Several species of Solanum produce secondary metabolites with antimicrobial activity. In the present study, the inhibitory activity of Solanum chrysotrichum, S. erianthum, S. torvum and S. rostratum against phytopathogenic Curvularia lunata was determined. Methanol extracts from roots, stems, leaves and fruits were evaluated by the method of mycelial inhibition on agar and the minimum inhibitory concentration (MIC) was determined on a liquid
medium. To increase the antimicrobial activity, the combined activity of the most active extracts for each phytopathogen was also determined (a combination of intra and interspecies extracts). The results showed that 12 of the 16 methanolic extracts of Solanum species had antifungal effects against C. lunata. The extracts of S. rostratum and S. erianthum developed the highest activity (~80% inhibition and 28.4 MIC μg . ml–1), even, equal to or greater than, the reference fungicide. The mixture of the active extracts of S. chrysotrichum and S. torvum increased their activity. Various extracts affected the macro and microscopicmorphology and most of them reduced the number of conidia of the fungus. This resulted in the capacity to control the vegetative growth and reproduction of C. lunata, the causal fungus of corn leaf spot disease.
CORRESPONDING AUTHOR:
Iván de la Cruz Chacón
Instituto de Ciencias Biológicas, Universidad de Ciencias y Artes de Chiapas, UNICACH, Libramiento Norte 1500. Col. Lajas Maciel, 29035 Tuxtla Gutierrez, Mexico
REFERENCES (16):
| 1. | Abhishek R.U., Thippeswamy S., Manjunath K., Mohana D.C. 2015. Antifungal and antimycotoxigenic potency of Solanum torvum Swartz. leaf extract: isolation and identification of compound active against mycotoxigenic strains of Aspergillus flavus and Fusarium verticillioides. Journal of Applied Microbiology 119 (6): 1624–1636. DOI: https://doi.org/10.1111/jam.12.... |
| 2. | Akram W., Anjum T., Ahmad A., Moeen R. 2014. First report of Curvularia lunata causing leaf spots on Sorghum bicolor from Pakistan. Plant Disease 98 (7): 1007. DOI: https://doi.org/10.1094/PDIS-1.... |
| 3. | Baka Z.A.M. 2014. Plant extract control of the fungi associated with different Egyptian wheat cultivars grains. Journal of Plant Protection Research 54 (3): 231–237. DOI: https://doi.org/10.2478/jppr-2.... |
| 4. | Bari M., Islam A., Khan W., Abul Mandal A.R. 2010. Antibacterial and antifungal activity of Solanum torvum (Solanaceae). International Journal of Agriculture and Biology 12 (3): 386–390. |
| 5. | Bassolé I.H.N., Juliani H.R. 2012 Essential oils in combination and their antimicrobial properties. Molecules 17 (4): 3989–4006. DOI: https://doi.org/10.3390/molecu.... |
| 6. | Chou S.C., Huang T.J., Lin E.H., Huang C.H., Chou C.H. 2012. Antihepatitis B virus constituents of Solanum erianthum. Natural Product Communications 7 (2): 153–156. |
| 7. | Das J., Lahan J.P., Srivastava R.B. 2010. Solanum melongena: A potential source of antifungal agent. Indian Journal of Microbiology 50 (Sup 1): 62–69. DOI: https://doi.org/10.1007/s12088.... |
| 8. | Eksteen D., Pretorius J.C., Nieuwoudt T.D., Zietsman P.C. 2001. Mycelial growth inhibition of plant pathogenic fungi by extracts of South African plant species. Annals of Applied Biology 139 (2): 243–249. DOI: https://doi.org/10.1111/j.1744.... |
| 9. | Essien E.E., Oqunwande I.A., Setzer W.N., Ekundayo O. 2012. Chemical composition, antimicrobial, and cytotoxicity studies on S. erianthum and S. macranthum essential oils. Pharmaceutical Biology 50 (4): 474–480. DOI: https://doi.org/10.3109/138802.... |
| 10. | Gao S., Li Y., Gao J., Suo Y., Fu K., Li Y., Chen J. 2014. Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus. BMC Genomics 15 (1): 627. DOI: https://doi.org/10.1186/1471-2.... |
| 11. | Hammer Ø., Harper D.A.T., Ryan P.D. 2001. PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4: 1–9. |
| 12. | Herrera-Arellano A., López-Villegas E.O., Rodríguez-Tovar A.V., Zamilpa A., Jiménez-Ferrer E., Tortoriello J., Martínez-Rivera M.A. 2013. Use of antifungal Saponin SC-2 of Solanum chrysotrichum for the treatment of vulvovaginal candidiasis: in vitro studies and clinical experiences. African Journal of Traditional, Complementary and Alternative Medicines 10 (3): 410–417. DOI: https://doi.org/10.4314/ajtcam.... |
| 13. | Koduru S., Grierson D.S., Afolayan A.J. 2006. Antimicrobial activity of Solanum aculeastrum. Pharmaceutical Biology 44 (4): 283–286. DOI: https://doi.org/10.1080/138802.... |
| 14. | Moussa S.H., Tayel A.A., Al-Hassan A.A., Farouk A. 2013. Tetrazolium/Formazan test as an efficient method to determine fungal chitosan antimicrobial activity. Journal of Mycology: 1–7. DOI: https://doi.org/10.1155/2013/7.... |
| 15. | Pérez-Amador M.C., Muñoz Ocotero V., García Castañeda J.M., González Esquinca A.R. 2007. Alkaloids in Solanum torvum Sw (Solanaceae). Phyton 76: 39–45. |
| 16. | Shabana Y.M., Abdalla M.E., Shahin A.A., El-Sawy M.M., Draz I.S., Youssif A.W. 2017. Efficacy of plant extracts in controlling wheat leaf rust disease caused by Puccinia triticina. Egyptian Journal of Basic and Applied Sciences 4 (1): 67–73. DOI: https://doi.org/10.1016/j.ejba.... |
