ORIGINAL ARTICLE
Aspergillus niger, a dominant phylloplane coloniser, influences the activity of defense enzymes in Solanum lycopersicum
| 1 | Amity Institute of Biotechnology, Amity University, Noida, India |
| 2 | Department of Botany, University of Delhi, New Delhi, India |
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Online publication date: 2019-12-18
Submission date: 2019-01-23
Acceptance date: 2019-06-18
Journal of Plant Protection Research 2019;59(4):512–518
KEYWORDS
Aspergillus nigerphylloplaneperoxidasephenylalanine ammonia lyasepolyphenol oxidaseSolanum lycopersicumtyrosine ammonia lyase
TOPICS
ABSTRACT
Phylloplane microbes have been studied as strategic tools in management against plant
pathogens. Non-pathogenic bacteria and fungi have been applied as crop protectants
against various plant diseases. The present study aimed at evaluating the potentiality of
Aspergillus niger spores in altering the activity of four key enzymes related to defense in
tomato. The experiment was designed such that two groups of 50 tomato plants were considered:
group 1 – sprayed with autoclaved distilled water (control) and group 2 – sprayed
with A. niger spores. Spraying was carried out under aseptic conditions. The experimental
parameters included analysis of the activity of peroxidase (POX), polyphenol oxidase
(PPO), phenylalanine ammonia lyase (PAL) and tyrosine ammonia lyase (TAL) as well
as expression of POX and PPO isoforms. The results demonstrated an inductive effect of
A. niger on the activity of POX, PPO, PAL and TAL. Enhanced expression of POX and PPO
isoforms was also observed. The results indicated that A. niger can be considered probiotic
for the management of tomato against its phytopathogens.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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