RAPID COMMUNICATION
Association of ’Candidatus Phytoplasma aurantifolia’ with Cosmos bipinnatus phyllody disease in Iran
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| 1 | Department of Agriculture, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran |
| 2 | Young Researcher and Elite Clubs, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran |
| 3 | Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran |
CORRESPONDING AUTHOR
Mehrnoosh Nikooei
Department of Agriculture, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran
Department of Agriculture, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran
Submission date: 2017-05-11
Acceptance date: 2017-08-31
Journal of Plant Protection Research 2017;57(3):314–317
KEYWORDS
TOPICS
ABSTRACT
In 2017 growing season numerous examinations of Cosmos bipinnatus in Hormozgan province, Iran revealed the disease symptoms similar to those associated with phytoplasmas. Phytoplasmas were detected from all symptomatic plants by the specific polymerase chain reaction (PCR) utilizing phytoplasma universal primer pairs. Amplification, sequencing and blast analysis of 16S rDNA fragment (ca. 1.2 kb) demonstrated that C. bipinnatus plants were infected by a phytoplasma belonging to the 16SrII group. This is the first report of association of a ‘Candidatus Phytoplasma aurantifolia’-related strain with C. bipinnatus phyllody in Iran.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (12)
1.
Chaturvedi Y., Rao G.P., Tewari A.K., Duduk B., Bertaccini A. 2010. Phytoplasma on ornamentals: detection, diversity and management. Acta Phytopathologica et Entomologica Hungarica 45 (1): 31–69. DOI: https://doi.org/10.1556/aphyt.....
2.
Chen W., Huang Y., Tsai M., Lin C. 2011. Detection and identification of a new associated with periwinkle leaf yellowing disease in Taiwan. Australas Plant Pathology 40 (5): 476–483. DOI: https://doi.org/10.1007/s13313....
3.
Deng S., Hiruki C. 1991. Amplification of 16Sr RNA genes from culturable and non-culturable mollicutes. Journal of Microbiological Methods 14 (1): 53–61. DOI: https://doi.org/10.1016/0167-7....
4.
Doyle J.J., Doyle J.L. 1990. A rapid total DNA preparation procedure for fresh plant tissue. Focus 12: 3–15.
5.
Firrao G., Gibb K., Streten C. 2005. Short taxonomic guide to the genus ‘Candidatus phytoplasma’. Journal of Plant Pathology 87 (4): 249–263.
6.
Gundersen D.E., Lee I.M. 1966. Ultrasensitive detection of phytoplasma by nested-PCR assays using two universal primer sets. Phytopathologia Mediterranea 35 (3): 144–151.
7.
Hemmati C., Nikooei M. 2017. Molecular characterization of a Candidatus Phytoplasma aurantifolia-related strain associated with Zinnia elegans phyllody in Iran. Australasian Plant Disease Notes 12 (1): 1–4. DOI: https://doi.org/10.1007/s13314....
8.
Rojas-Martinez R.I., Zavaleta-Mejia E., Martinez-Soriano J.P., Lee I.M. 2003. Identification of the phytoplasma associated with cosmos (Cosmos bipinnatus cav.) phyllody and classification by RFLP analysis of 16S rDNA. Revista Mexicana de Fitopathologia 21 (1): 83–86.
9.
Schneider B., Seemuller E., Smart C.D., Kirkpatrick B.C. 1995. Phylogenetic classification of plant pathogenic mycoplasmalike organism or phytoplasma. p. 369–380. In: “Molecular and Diagnostic Procedures in Mycoplasmology” (S. Razin, J.G. Tully, eds.) Academic Press, New York, USA.
10.
Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. DOI: https://doi.org/10.1093/molbev....
11.
Wang K., Hiruki C. 2001. Use of heteroduplex mobility assay for identification and differentiation of phytoplasmas in the aster yellows group and the clover proliferation group. Phytopathology 91 (6): 546–552. DOI: https://doi.org/10.1094/phyto.....
12.
Zhao Y., Wei W., Lee I.M., Shao J., Suo X.B., Davis R.E. 2009. Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology 59 (10): 2582–2593. DOI: https://doi.org/10.1099/ijs.0.....
