ORIGINAL ARTICLE
Burkholderia sp. strain TNAU-1 for biological control of root rot in mung bean (Vigna radiata L.) caused by Macrophomina phaseolina
1
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University
Coimbatore – 641 003, Tamil Nadu, India
Corresponding author
Rethinasamy Velazhahan
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University Coimbatore – 641 003, Tamil Nadu, India
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University Coimbatore – 641 003, Tamil Nadu, India
Journal of Plant Protection Research 2011;51(3):273-278
KEYWORDS
TOPICS
ABSTRACT
The potential of
Burkholderia
sp. strain TNAU-1
for the management of mung bean (
Vigna radiata
L.) root rot caused by
Macrophomina phaseolina
was evaluated under greenhouse conditions.
Burkholderia
sp. strain TNAU-1 inhibited the mycelial growth
of
M. phaseolina
in vitro
and produced an inhibition zone of 18.8 mm. Mung bean seeds when treated with the bacterial suspension,
showed significant increase in root length, shoot length and seedling vigour. A talc-based powder formulation of
Burkholderia
sp.
strain TNAU-1
was developed and evaluated for its efficacy in the management of mung bean root rot under greenhouse conditions.
Seed treatment or soil application of the powder formulation of
Burkholderia
sp. strain TNAU-1
significantly reduced the incidence of
root rot and increased the germination percentage and plant height. Seed treatment with the powder formulation of
Burkholderia
sp.
strain TNAU-1
alone was effective in controlling root rot disease; but the combined seed treatment and soil application of
Burkholderia
sp. strain TNAU-1, increased the efficacy. Seed treatment and soil application with
Burkholderia
sp. reduced the root rot incidence from
52.6 per cent (with non-bacterized seeds) to 16.7 per cent. Control of root rot with the application of
Burkholderia
sp. by seed treatment
and soil application was not statistically different from that obtained with seed treatment with carbendazim. The endophytic move-
ment of
Burkholderia
sp. in the stem, roots and leaves of mung bean was confirmed through PCR using
Burkholderia
sp. specific primers
which resulted in the amplification of a 417 bp product.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (36)
1.
Baki A.A.A., Anderson J.D. 1973. Vigour determination in soybean seed by multiple criteria. Crop Sci. 31: 630–633.
2.
Baligh M., Delgado M.A., Conway K.E. 1999. Evaluation of Burkholderia cepacia strains: Root colonization of Catharanthus roseus and in vitro inhibition of selected soil-borne fungal pathogens. Proc. Oklahama Acad. Sci.79: 19–27.
3.
Cain C.C., Henry A.T., Waldo R.H., Casida L.J., Falkinham J.O. 2000. Identification and characteristics of a novel Burkholderia strain with broad-spectrum antimicrobial activity. Appl. Environ. Microbiol. 66: 4139–4141.
4.
Chen C., Bauske E.M., Musson G., Rodriguez-Kabana R., Kloepper J.W. 1995. Biological control of Fusarium wilt on cotton by use of endophytic bacteria. Biol. Control 5: 83–91.
5.
Dennis C., Webster J. 1971. Antagonistic properties of species groups of Trichoderma 1. Production of non-volatile antibiotics. Trans. Br. Mycol. Soc. 57: 25–39.
6.
Dhingra O.B., Sinclair J.B. 1978. Biology and Pathology of Macrophomina phaseolina. Universidade Federal de Viscosa, Brazil, 166 pp.
7.
Dikin A., Sijam K., Abidin M.A.Z., Idris A.S. 2003. Biological control of seedborne pathogen of oil palm, Schizophyllum commune Fr. with antagonistic bacteria. Int. J. Agric. Biol. 5: 507–512.
8.
Gomez K.A., Gomez A.A. 1984. Statistical Procedure for Agricultural Research.Wiley, New York, NY, USA, 680 pp.
9.
Hebbar K.P., Martel M.H., Heulin T. 1998. Suppression of pre and post-emergence damping-off in corn by Burkholderia cepacia. Eur. J. Plant Pathol.104: 29–36.
10.
Heungens K., Parke J.L. 2000. Zoospore homing and infection events: Effects of the biocontrol bacteria Burkholderia cepacia AMMDR1 on two oomycete pathogen of pea (Pisum sativum L.). Appl. Environ. Microbiol. 66: 192–200.
11.
Heydari A., Misaghi I.J. 1998. Biocontrol activity of Burkholderia Cepacia against Rhizoctonia solani in herbicide-treated soils. Plant Soil 202: 109–116.
12.
Hwang J., Benson D.M. 2002. Biocontrol of Rhizoctonia stem and root rot of poinsettia with Burkholderia cepacia and binucleate Rhizoctonia. Plant Dis. 86: 47–53.
13.
Indira N., Gayatri S. 2003. Management of blackgram root rot caused by Macrophomina phaseolina by antagonistic microorganisms. Madras Agric. J. 90: 490–494.
14.
International Seed Testing Association. 1996. International Rules for Seed Testing. Seed Sci. Technol Supp. 24: 155–202.
15.
Janisewiez W.J., Roitmann J. 1988. Biological control of blue mold and gray mold on apple and pear with Pseudomonas cepacia. Phytopathology78, p. 1697.
16.
Karthikeyan M., Bhaskaran R., Radhika K., Mathiyazhahan S., Jayakumar V., Sandosskumar R., Velazhahan R. 2005. Endophytic Pseudomonas fluorescens Endo2 and Endo35 induce resistance in black gram [Vigna mungo(L.) Hepper] to the pathogen Macrophomina phaseolina. J. Plant Interact. 1: 135–143.
17.
King E.B., Parke J.L. 1993. Biocontrol of Aphanomyces root rot and Pythium damping-off by Pseudomonas cepacia AMMD on four pea cultivars. Plant Dis. 77: 1185–1188.
18.
Li W., Roberts D.P., Dery P.D., Meyer S.L.F., Lohrke S., Lumsden R.D., Hebbar K.P. 2002. Broad spectrum antibiotic activity and disease suppression by the potential biocontrol agent Burkholderia ambifaria BC-F. Crop Protect.21: 129–135.
19.
Lievens K.H., Rijsbergen V.R., Leyns F.R., Lambert B.J., Tenning P., Swings J., Joos J.P. 1989. Dominant rhizosphere bacteria as a source of antifungal agents. Pestic. Sci. 27: 141–154.
20.
Meyer J.M., Hohnadel D., Halle F. 1989. Cepabactin from Pseudomonas cepacia, a new type of siderophores. J. Gen. Microbiol.135: 1479–1487.
21.
Muthomi J.W., Otieno P.E., Cheminingwa G.N., Nderitu J.H., Wagachaja J.M. 2007. Effect of legume root rot pathogens and fungicide seed treatment on nodulation and biomass accumulation. J. Biol. Sci. 7: 1163–1170.
22.
Parke J.L., Rand R.E., Joy A.E., King E.B. 1991. Biological control of Pythium damping-off and Aphanomyces root rot of peas by application of Pseudomonas cepacia or Pseudomonas fluorescens to seed. Plant Dis. 75: 987–992.
23.
Raguchander T., Samiyappan R., Arjunan G. 1993. Biocontrol of Macrophomina root rot of mungbean. Indian Phytopathol.46: 379–382.
24.
Rajendran L., Samiyappan R. 2008. Endophytic Bacillus species confer increased resistance in cotton against damping off disease caused by Rhizoctonia solani. Plant Pathol. 7: 1–12.
25.
Rettinassababady C., Ramadoss N., Thirumeni S. 2002. Management of root rot [Macrophomina phaseolina(Tassi.) Goid] of rice fallow blackgram by resident isolates of Trichoderma viride. Indian J. Agric. Res. 36: 118–122.
26.
Riker A. J., Riker R. S. 1936. Introduction to Research on Plant Disease. John S. Swift Co., INC., New York, 117 pp.
27.
Saravanakumar D., Harish S., Loganathan M., Vivekananthan R., Rajendran L., Raguchander T., Samiyappan R. 2007. Rhizobacterial formulation for the effective management of Macrophomina root rot in mungbean. Arch. Phytopathol. Plant Protect. 40: 323–337.
28.
Sijam K., Dikin A. 2005. Biochemical and physiological characterization of Burkholderia cepacia as biological control agent. Int. J. Agri. Biol. 7: 385–388.
29.
Su G., Suh S.O., Schneider R.W., Rusin J.S. 2001. Host specialization in the charcoal rot fungus, Macrophomina phaseolina. Phytopathology 92: 120–126.
30.
Suparman M., Kamaruzaman S., Sariah M., Inon S. 2002. In vitro Screening of Antagonistic Bacteria against Fusarium oxysporum f.sp. lycopersici. Programme and abstracts 25th Malaysian Microbiology Symposium and 5th UNESCO National Workshop for the Promotion of Microbiology in Malaysia, 24 pp.
31.
Taylor S.R., Weaver B.D., Wood W.C., van Edzard S. 2005. Nitrogen application increases yield and early dry matter accumulation in late-planted soybean crop. Sci. J. 45: 854–858.
32.
Thilagavathi R., Saravanakumar D., Ragupathi N., Samiyappan R. 2007. A combination of biocontrol agents improves the management of dry root rot (Macrophomina phaseolina) in greengram.Phytopathol. Mediterr. 46: 157–167.
33.
Thompson D.C. 1996. Evaluation of bacterial antagonist for reduction of summer patch symptoms in Kentucky blue grass. Plant Dis. 80: 856–862.
34.
Vijayasamundeeswari A., Vijayanandraj S., Paranidharan V., Samiyappan R., Velazhahan R. 2010. Integrated management of aflatoxin B1contamination of groundnut (Arachis hypogaea L.) with Burkholderia sp. and zimmu (Allium sativum L. xAllium cepa L.) intercropping.J. Plant Interact. 5: 59–68.
35.
Watanabe T., Tsukamoto T., Shirata A. 2000. Potential of antagonistic isolate obtained from Lentinus lepideus basidiospores as a biocontrol agent. Mycoscience 41:79–82.
36.
Wyllie T.D. 1993. Charcoal Rot. p. 30–33. In: “Compendium of Soybean Diseases” (J.B. Sinclair, P.A. Backman, eds). 3rd ed. The APS Press. St. Paul, MN, 106 pp.
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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