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Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris' subgroup rpI-L in Iran
 
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Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
CORRESPONDING AUTHOR
Leila Zirak
Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Submission date: 2013-12-14
Acceptance date: 2014-05-30
 
Journal of Plant Protection Research 2014;54(2):199–203
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ABSTRACT
In two of Iran’s central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect ‘Canididatus Phytoplasma asteris’-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR) assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/R16R2 in nested PCR. Then, for detection of ‘Ca. P. asteris’, DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp 509I restriction enzyme as well as sequence analyses indicated that ‘Ca. P. asteris’-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rp I-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rp I-L subgroup.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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