ORIGINAL ARTICLE
Components of quantitative resistance in barley plants to Fusarium head blight infection determined using three in vitro assays
Nachaat Sakr 1  
 
 
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Department of Agriculture, Atomic Energy Commission of Syria, 17th Nissan St. – Kafersouseh, 6091 Damascus, Syria
Submission date: 2017-10-26
Acceptance date: 2018-02-27
 
Journal of Plant Protection Research 2018;58(2):176–183
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ABSTRACT:
Quantitative resistance in barley to four Fusarium head blight (FHB) species was investigated in vitro. Nine components involved in three assays (detached leaf, modified Petri-dish and seedling tests) were compared on two widely grown Syrian barley cultivars: Arabi Aswad (AS) and Arabi Abiad (AB). On AB, inoculation with FHB species resulted in significantly shorter latent period, larger lesion length of detached leaf inoculation, more AUDPC standard and higher percentage of infected seedlings of pin-point inoculation compared with AS. Latent period of AB was 14.89% less than AS, lesion length of AB was 6.01% less than AS, AUDPC standard of AS was less 17.07% than AB and percentage of infected seedlings of AS was 4.87% less than AB. Inoculation with FHB species resulted in no significant differences in other five components measured: incubation period, lesion length of clip-dipping inoculation, germination rate reduction, coleoptile length reduction, and percentage of infected seedlings of foliar-spraying inoculation. AS was more resistant to in vitro FHB infection than AB. Latent period and AUDPC standard recorded the highest values compared with the lowest values for lesion length and percentage of infected seedlings. It seems that measurement of latent period and AUDPC standard may be useful in identifying barley cultivars highly susceptible or resistant to FHB at early stages.
CORRESPONDING AUTHOR:
Nachaat Sakr   
Agriculture, Atomic Energy Commission of Syria, 17th Nissan St. – Kafersouseh, 6091 Damascus, Syria
 
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