Development of mutants of Coniothyrium minitans with improved efficiency for control of Sclerotinia sclerotiorum
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Brazilian Agricultural Research Corporation-Embrapa Environment, C.P.69, 13820-000, Jaguariúna-SP, Brazil
Departament of Biology, Faculdade de Engenharia, UNESP, C.P.31, 15385-000, Ilha Solteira-SP, Brazil
School of Biological Sciences, Birkbeck College, Malet Street, London WC1E 7HX, London, UK
Itamar S. Melo
Brazilian Agricultural Research Corporation-Embrapa Environment, C.P.69, 13820-000, Jaguariúna-SP, Brazil
Journal of Plant Protection Research 2011;51(2):179–183
Coniothyrium minitans (CM) is hyperparasitic to Sclerotinia sclerotiorum (SS), a pathogen of many economically important crops. In this paper, we describe the isolation of improved mutants of CM, using a UV – irradiation regime, with altered chitinase production and tolerance to high concentration of iprodione, which are effective against SS. Three out of the 59 mutants obtained inhibited the mycelial growth of CM. Infectivity of sclerotia by the new mutants was assayed by the plant-tissue-based system using carrot segments. More than 80% of sclerotia were colonized by the mutants and the wild-type CM. The mutant strains retained ability to produce significant amounts of chitinase. The mutants differed from their wild-type strain in appearance, morphology and sporulation. In conclusion, the results presented here provide evidence that the new biotypes of C. minitans are effective in controlling S. sclerotiorum
The authors have declared that no conflict of interests exist.
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