ORIGINAL ARTICLE
Effect of amino acid application on induced resistance against citrus canker disease in lime plants
 
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1
Department of Plant Protection, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran
2
Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran
3
Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University G.C., 1194968319, Evin, Tehran, Iran
Submission date: 2013-12-11
Acceptance date: 2014-05-07
 
Journal of Plant Protection Research 2014;54(2):144–149
KEYWORDS:
TOPICS:
ABSTRACT:
Citrus bacterial canker, caused by Xanthomonas citri subsp. citri (Xcc), is a destructive disease. So far, the chemicals used to control this pathogen are either ineffective or harmful to the environment. To improve control of this disease, lime (Citrus aurantifolia) were treated with L-arginine, L-methionine, L-ornithine, and distilled water. Plants were inoculated with Xcc, 48 hours post treatment. Lesion diameters of inoculated leaves were evaluated four weeks after inoculation with a bacterial suspension. Changes in β-1,3-glucanase transcript levels and activity of antioxidant enzymes, catalase, peroxidase, and phenylalanine ammonia-lyase were investigated at 48 hours post treatment and 24, 48, and 72 hours post inoculation. Based on the results of phenotypic, antioxidant enzyme activity and a molecular study of the stressed plants, it was found that those plants treated with the amino acid methionine significantly increased the plant induced resistance as well as decreased the severity of disease by reducing necrotic lesion size.
CONFLICT OF INTERESTS:
The authors have declared that no conflict of interests exist.
CORRESPONDING AUTHOR:
Vahideh Hasabi
Department of Plant Protection, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran
 
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