Effect of fungicides on colony growth of Colletotrichum lindemuthianum (Sacc. & Magn.) Scrib.
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UNESP – Paulista State University, Faculty of Agronomy Department of Crop Production P.O. Box 237, 186.03-970 Botucatu, State of São Paulo, Brazil
Maringoni Antonio Carlos
UNESP – Paulista State University, Faculty of Agronomy Department of Crop Production P.O. Box 237, 186.03-970 Botucatu, State of São Paulo, Brazil
Journal of Plant Protection Research 2008;48(2):201–212
Colletotrichum lindemuthianum (Sacc. & Magn.) Scrib. is the causal agent of the anthracnose of common bean (Phaseolus vulgaris L.), a fungal disease of a great significance in brazilian bean cultures. The goals of this work were to evaluate the in vitro colony growth and to determine the ED50 interval of twenty C. lindemuthianum isolates from different regions of Brazil to five fungicides of different active ingredients and to some blendings (carbendazim, chlorothalonil, thiophanate-methyl, chlorothalonil + thiophanate-methyl, trifloxystrobin, propiconazole and trifloxystrobin + propiconazole), at concentrations of 0, 1, 10, 100 and 1 000 μg/ml, in a potato-dextrose-agar culture medium. The results revealed seven isolates with low sensitivity to carbendazim and thiophanate-methyl (ED50 interval greater than 1000 μg/ml) thus suggesting cross-resistance. Isolate sensitivity to chlorothalonil ranged from ED50 interval less than 1 μg/ml to greater than 1 000 μg/ml. Those isolates with high sensitivity to thiophanate-methyl, ED50 interval less than 1 μg/ml, did also show it with respect to chlorothalonil + thiophanate-methyl. Sixteen isolates showed a high sensitivity to trifloxystrobin with a ED50 interval less than 1 μg/ml. Nineteen isolates of C. lindemuthianum showed high sensitivity to propiconazole and to trifloxystrobin + propiconazole with ED50 interval less than 1 μg/ml. Isolates with low sensitivity to carbendazim and thiophanate-methyl were sensitive to propiconazole and to trifloxystrobin + propiconazole. Variability was found in the sensitivity of the colony growth of C. lindemuthianum isolates from different regions of Brazil to the fungicides evaluated.
The authors have declared that no conflict of interests exist.
1. Agrofit 2007. http://extranet.agricultura.go....
2. Azevedo L.A.S. 2003. Fungicidas Protetores Fundamentos para o Uso Racional. Luís Antônio Siqueira de Azevedo, São Paulo, Brazil, 318 pp.
3. Balardin R.S., Rodrigues J.C.V. 1995. Sensibilidade “in vitro” de raças de Colletotrichum lindemuthianum a fungicidas sistêmicos e protetores. Fitopatol. Bra. 20 (3): 494–497.
4. Canteri M.G., Dalla Pria M., Silva O.C. 1999. Principais Doenças Fúngicas do Feijoeiro: Orientações para Manejo Econômico e Ecológico. UEPG, Ponta Grossa, Brazil, 178 pp.
5. Ghini R., Kimati H. 2000. Resistência de Fungos a Fungicidas. Embrapa Meio Ambiente, Jaguariúna, Brazil, 78 pp.
6. Gianasi L. 2002. Patossistema feijoeiro-antracnose: efeito do trifenil acetato de estanho no crescimento do hospedeiro e no progresso da doença. Cienc. Agrotecnol. 6 (2): 309–317.
7. Dekker J. Georgopoulos S.G. 1982. Fungicide Resistance in Crop Protection. Center for Agricultural Publishing and Documentation, Wageningen, The Netherlands, 265 pp.
8. Kopacki M., Wagner A. 2006. Effect of some fungicides on mycellian growth of Fusarium avenaceum (Fr.) Sacc. pathogenic to chrysanthemum (Dendranthena grandiflora Tzvelev). Agronom. Res. 4 (Special issue): 237–240.
9. Maringoni A.C., Barros E.M. 2002. Ocorrência de isolados de Colletotrichum lindemuthianum resistentes a fungicidas benzimidazóis. Summa Phytopathol. 28 (2): 197–200.
10. Mckay G.J., Cook L.R. 1997. A PCR – based method to characterize and identify benzimidazole resistance in Helminthosporium solani. FEMS Microbiol. Lett. 152: 371–378.
11. Mckay G.J., Egan D., Morris E., Brown A. E. 1998. Identification of benzimidazole resistance in Cladobotrium dendroides using PCR based method. Mycol. Res. 102 (6): 671–676.
12. Oliveira S.H.F. 2003. Novos fungicidas e programas de pulverização para o controle da antracnose e da mancha angular do feijoeiro. Summa Phytopathol. 29 (1): 45–48.
13. Rava C.A. 2002. Eficiência de fungicidas no controle da antracnose da mancha angular do feijoeiro comum. Summa Phytopathol. 28 (1): 65–69.
14. Rava C.A., Sartorato A., Botelho S.A. 1998. Eficiência in vitro e in vivo de fungicidas no controle de Colletotrichum lindemuthianum. Summa Phytopathol. 24 (1): 45–48.
15. Sartorato A. 2006. Sensibilidade ‘in vitro’ de isolados de Colletotrichum lindemuthianum a fungicidas. Pesqui. Agropecu. Trop. 36 (3): 211–213.
16. Shepers H.T.A.M. 1985. Changes during a three-year period in the sensitivity to ergosterol biosynthesis inhibitors of Sphaerotheca fuliginea in the Netherlands. Neth. J. Plant Pathol. 91: 105–118.
17. Smith F.D. 1989. Baseline-sensitivity of three populations of Venturia inaequalis to fluzilazole. Phytopathology 79: 1171.
18. Tozze Junior H., Mello M.B.A., Massola Junior N.S. 2006. Caracterização morfológica e fisiológica de Colletotrichum sp causadores de antracnose em solanáceas. Summa Phytopathol. 32 (1):77–79.
19. Waard M.A.de, Kipp E.M.C., Horn N.M., Nistelrooy J.G.M.van. 1986. Variation in sensitivity to fungicides which inhibit ergosterol biosynthesis in wheat powdery mildew. Neth. J. Plant Pathol. 92 (1): 21–23.
20. Wu X.Q., Hong Y.D. 2000. Variation of chlorotahlonil sensitivity within Sphaeropis sapinea in China. J. Nanjing Forest. Univ. 24 (4): 16–20.