ORIGINAL ARTICLE
Effects of a lectin from Polygonum persicaria L. on Pieris brassicae L. (Lepidoptera: Pieridae)
 
More details
Hide details
1
Department of Plant Protection, Faculty of Agricultural Science, University of Guilan, P.O. Box 41635–1314, Rasht, Iran
CORRESPONDING AUTHOR
Arash Zibaee
Department of Plant Protection, Faculty of Agricultural Science, University of Guilan, P.O. Box 41635–1314, Rasht, Iran
Submission date: 2014-02-13
Acceptance date: 2014-07-22
 
Journal of Plant Protection Research 2014;54(3):250–257
KEYWORDS
TOPICS
ABSTRACT
In the current study, the entomotoxic effects of a lectin, extracted from Polygonum persicaria, were determined on Pieris brassicae larvae. The purified molecule significantly decreased larval survival, weight, and mass protein. Different concentrations of P. persicaria agglutinin (PPA) (0.5, 1 and 2 mg/ml) significantly decreased nutritional indices including: the efficiency of conversion of ingested food (ECI), the efficiency of conversion of digested food (ECD), and approximate digestibility (AD) in a dose-dependent manner. There was a significant decrease in larval α-amylase activity after larval fed on PPA. The decreased activity was found in both the biochemical assessment and gel electrophoresis; sharpness of one isozyme decreased and another completely disappeared. Meanwhile, PPA significantly decreased activities of glucosidases mainly β-glucosidase. Fed of P. brassicae larvae on different concentrations of PPA, significantly decreased activity of TAG-lipase in both the biochemical assessment and gel electrophoresis. It was found that alkaline phosphatase (ALP) and acid phosphatase (ACP) were also affected by PPA but concentrations of 1 and 2 mg/ml showed statistical differences compared to the control. General and specific proteolytic activities statistically decreased after larvae fed on different concentrations of PPA. Results of our study could bring attention to this molecule, showing it to be a safe and efficient control of the pest worldwide.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (31)
1.
Bernfeld P. 1955. Amylases, α and β. Methods Enzymol. 1: 149–158.
 
2.
Bulgakov A.A., Parka K., Choia K.S., Limb H.K., Cho M. 2004. Purification and characterisation of a lectin isolated from the Manila clam Ruditapes philippinarum in Korea. Fish Shellfish Immunol. 16 (4): 487–499.
 
3.
Campos F.A.P., Xavier-Filho J., Silva C.P., Ary M.B. 1989. Resolution and partial characterization of proteinases and α-amylases from midgut of larvae of the bruchid beetle Callosobruchus maculatus (F.). Comp. Biochem. Physiol. Part B. 92 (1): 51–57.
 
4.
Carlini C.R., Grossi-de-Sa M.F. 2002. Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides. Toxicon 40 (11): 1515–1539.
 
5.
Coelho M.B., Marangoni S., Macedo M.L.R. 2007. Insecticidal action of Annona coriacea lectin against the flour moth Anagasta kuehniella and the rice moth Corcyra cephalonica (Lepidoptera: Pyralidae). Comp. Biochem. Physiol. Part C. 146 (3): 406–414.
 
6.
Cohen A.C. 1993. Organization of digestion and preliminary characterization of salivary trypsin-like enzymes in a predaceous heteropteran, Zelus renardii. J. Insect Physiol. 39 (10): 823–829.
 
7.
Correia M.T.S., Coelho L.C.B.B. 1995. Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis Mart. (Camaratu bean). Appl. Biochem. Biotechnol. 55 (3): 261–273.
 
8.
de Oliveira C.F.T., Luz L.A., Paiv P.M.G., Coelho L.C.B.B., Marangoni S., Macedo M.L.R. 2011. Evaluation of seed coagulant Moringa oleifera lectin (cMoL) as a bioinsecticidal tool with potential for the control of insects. Process Biochem. 46 (2): 498–504.
 
9.
Fitches E.C., Gatehouse A.M.R., Gatehouse J.A. 1997. Effects of snowdrop lectin (GNA) delivered via artificial diet and transgenic plants on the development of tomato moth (Lacanobia oleracea) larvae in laboratory and glasshouse trials. J. Insect Physiol. 43 (8): 727–739.
 
10.
Folin O., Ciocalteu V. 1927. On tyrosine and tryptophane determinations in proteins. J. Biol. Chem. 73: 627–650.
 
11.
Garcia-Carreno F.L., Dimes L.E., Haard N.F. 1993. Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous protease inhibitors. Anal. Biochem. 214 (1): 61–69.
 
12.
Hamshou M., Smagghe G., Van Damme E.J.M. 2010. Entomotoxic effects of fungal lectin from Rhizoctonia solani towards Spodoptera littoralis. Fungal Biol. 114 (1): 34–40.
 
13.
Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
 
14.
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.
 
15.
Macedo M.L.R., Damico D.C., Freire M.G.M., Toyama M.H., Marangoni S., Novello J.C. 2003. Purification and characterization of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effect on the larval development of Callosobruchus maculatus (Coleoptera: Bruchidae) and Anagasta kuehniella (Lepidoptera: Pyralidae). J. Agric. Food Chem. 51 (10): 2980–2986.
 
16.
Macedo M.L.R., Freire M.G.M., Silva M.B.R., Coelho L.C.B.B. 2007. Insecticidal action of Bauhinia monandra leaf lectin (BmoLL) against Anagasta kuehniella (Lepidoptera: Pyralidae), Zabrotes subfasciatus and Callosobruchus maculatus (Coleoptera: Bruchidae). Comp. Biochem. Physiol. Part A. 146 (4): 486–498.
 
17.
Michiels K., Van Damme E.J.M., Smagghe G. 2010. Plant-insect interactions: what can we learn from plant lectins? Arch. Insect Biochem. Physiol. 73 (4): 193–212.
 
18.
Oppert B., Kramer K.J., McGaughey W.H. 1997. Rapid microplate assay of proteinase mixtures. BioTechnol. 23 (1): 70–72.
 
19.
Peumans W.J., Van Damme E.J.M. 1995. Lectins as plant defense proteins. Plant Physiol. 109 (2): 347–352.
 
20.
Powell K.S., Gatehouse A.M.R., Hilder V.A., Gatehouse J.A. 1995. Antifeedant effects of plant lectins and an enzyme on adult stage of the rice brown plant hopper, Nilaparvata lugens. Entomol. Experim. Appl. 75 (1): 51–59.
 
21.
Pusztai A., Bardocz S. 2009. Biological effects of plant lectins on the gastrointestinal tract: metabolic consequences and applications. Trends Glycosci. Glycotechnol. 8 (41): 149–165.
 
22.
Ramzi S., Sahragard A., Sendi J.J., Aalami A. 2013. Effects of an extracted lectin from Citrullus colocynthis L. (Cucurbitaceae) on survival, digestion and energy reserves of Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae). Front. Physiol. 12 (4): 328. DOI: 10.3389/fphys.2013.00328.
 
23.
Sadeghi A., Smagghe G., Proost P., Van Damme E.J.M. 2008. Ferritin acts as a target site for the snowdrop lectin (GNA) in the midgut of the cotton leafworm Spodoptera littoralis. Insect Sci. 15 (6): 513–519.
 
24.
Sauvion N., Rahbe Y., Peumans W.J., Van Damme E.J.M., Gatehouse J.A., Gatehouse A.M.R. 1996. Effects of GNA and other mannose binding lectins on development and fecundity of the peach-potato aphid Myzus persicae. Entomol. Experim. Appl. 79 (3): 285–293.
 
25.
Scriber J.M., Slansky Jr.F. 1982. The nutritional ecology of immature insects. Ann. Rev. Entomol. 26 (1): 183–211.
 
26.
Simmonds N.W. 1945. Polygonum persicaria L. J. Ecol. 33: 121–131.
 
27.
Tinjuangjun P., Loc N.T., Gatehouse A.M.R., Gatehouse J.A., Christou P. 2000. Enhanced insect resistance in Thai rice varieties generated by particle bombardment. Mol. Breed. 6 (4): 391–399.
 
28.
Tsujita T., Ninomiya H., Okuda H. 1989. p-Nitrophenyl butyrate hydrolyzing activity of hormone-sensitive lipase from bovine adipose tissue. J. Lipid. Res. 30 (7): 997–1004.
 
29.
Van Damme E.J.M., Lannoo N., Peumans W.J. 2008. Plant lectins. Adv. Botan. Res. 48: 109–209. DOI: 10.1016/50065-2296(08)00403-5.
 
30.
Vasconcelos I.M., Oliveira J.T.A. 2004. Antinutritional properties of plant lectins. Toxicon 44 (4): 385–403.
 
31.
Zibaee A. 2011. Digestive enzymes of large cabbage white butterfly, Pieris brassicae L. (Lepidoptera: Pieridae) from developmental and site of activity perspectives. Italian J. Zool. 79 (1): 13–26.
 
eISSN:1899-007X
ISSN:1427-4345