ORIGINAL ARTICLE
Effects of inoculation with a commercial microbial inoculant Bacillus subtilis C-3102 mixture on rice and barley growth and its possible mechanism in the plant growth stimulatory effect
Yuki Koyama 1, D
,  
Thida Aye Win 1, B
,  
Koki Toyota 1, C-D,F  
,  
Seiya Chikamatsu 1, B
,  
Takeshi Shirai 2, A
,  
Taisuke Uesugi 2, A-B
,  
Hiroaki Murakami 2, A
,  
Tetsuya Ishida 2, A
,  
 
 
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1
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Japan
2
Process Development Laboratories, Asahi Group Holdings, Japan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Koki Toyota   

Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Naka, 184-8588, Koganei, Japan
Online publish date: 2019-06-27
Submission date: 2018-12-25
Acceptance date: 2019-06-04
 
Journal of Plant Protection Research 2019;59(2):193–205
KEYWORDS
TOPICS
ABSTRACT
The effects of a microbial inoculant (Thervelics®: a mixture of cells of Bacillus subtilis C-3102 and carrier materials) on rice (Oryza sativa cv. Milkyprincess) and barley (Hordeum vulgare cv. Sachiho Golden) were evaluated in four pot experiments. In the first and second experiments, the dry matter production of rice and barley increased significantly by 10–20% with the inoculation of the mixture at a rate of 107 cfu ⋅ g–1 soil compared with the non-inoculated control. In the third experiment, the growth promoting effects of the mixture, the autoclaved mixture and the carrier materials were compared. The dry mater production of rice grains was the highest in the mixture, and it was significantly higher in the three treatments than in the control, suggesting that the carrier materials may also have a plant growth promoting effect and the living cells might have an additional stimulatory effect. To confirm the efficacy of the living cells in the mixture, only B. subtilis C-3102 cells were used in the fourth experiment. In addition, to estimate the mechanisms in growth promotion by B. subtilis C-3102, three B. subtilis strains with similar or different properties in the production of indole-3-acetic acid (IAA), protease and siderophore and phosphatesolubilizing ability were used as reference strains. Only B. subtilis C-3102 significantly increased the dry matter production of rice grains and the soil protease activity was consistently higher in the soil inoculated with B. subtilis C-3102 throughout the growing period. These results indicate that the microbial inoculant including live B. subtilis C-3102 may have growth promoting effects on rice and barley.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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