The efficiency of a formulated salicylic acid (Zacha 11, 500 mg · l–1) and a Bacillus bioproduct (JN2-007, 1 × 107 cfu · ml–1) in controlling cassava root rot disease and enhancing growth was evaluated. The results revealed that cassava stalk soaking and foliage spraying with Zacha 11 formulation or Bacillus subtilis bioproduct could increase cassava growth at 60 days after planting under greenhouse conditions. Zacha 11 gave the tallest stem height (11.67 cm), the longest root length (18.91 cm) and the greatest number of roots (49.50). Fusarium root rot severity indices of all treated treatments were reduced, and were significantly lower than that of the water control. Plants treated with Zacha 11 and JN2-007 had disease severity reduction of 53.33 and 48.33%, respectively. Furthermore, all treatments increased the endogenous salicylic acid (SA) content in cassava plants at 24 inoculation with significant differences when compared to the untreated samples. The efficacy of Zacha 11 and JN2-007 was evaluated at two field locations, using two different cassava varieties, cv. Rayong 72 and CMR-89. The results showed that all elicitors could suppress root rot disease as well as bacterial leaf blight. Furthermore, the elicitors helped cassava plants cv. Rayong 72 and CMR-89 to increase tuber weight, yield and starch contents, compared to the water control. Thus, it is possible that these formulations could be effective in controlling diseases and increasing cassava productivity.
The authors would like to express their thanks to the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission. Partial funding was given by the Research and Researcher for Industries (RRi), the Thailand Research Funds for Ph.D. program number PHD58I0070 for Mr. Chanon Saengchan. We also would like to sincerely thank the Plant Pathology Laboratory, Suranaree University of Technology, research assistants for technical assistance, and graduate students for their being very supportive in terms of experimental materials.
The authors would like to express their thanks to the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission. Partial funding was given by the Research and Researcher for Industries (RRi), the Thailand Research Funds for Ph.D. program number PHD58I0070 for Mr. Chanon Saengchan.
Andrea Toledo
The authors have declared that no conflict of interests exist.
Buensanteai N., Yuen G.Y. Prathuangwong S. 2009. Priming, signaling, and protein production associated with induced resistance by Bacillus amyloliguefaciens KPS46. World Journal of Microbiology & Biotechnology 25: 1275–1286.
Camila S.H., Mariana P.S., Luiz R.C.J., de Eder J.O., de Saulo A.S.O. 2018. Modelling growth characteristics and aggressiveness of Neoscytalidium hyalinum and Fusarium solani associated with black and dry root rot diseases on cassava. Tropical Plant Pathology 43: 422–432.
Chaisinboon O., Chontanawat J. 2011. Factors determining the competing use of Thailand’s cassava for food and fuel. 9th Eco-Energy and Materials Science and Engineering Symposium. Energy Procedia 9 (2): 216–229.
Charaensatapon R., Saelee T., Chulkod U., Cheadchoo S. 2014. Phytophthora root and tuber of cassava in Thailand. Field and renewable energy crops research institute. Department of agriculture, Thailand. Proceedings of the 5th Asian Conference on Plant Pathology. 3–6 November, Chiang Mai, Thailand.
Chávez-Arias C.C., Gómez-Caro S., Restrepo-Díaz H. 2020. Physiological responses to the foliar application of synthetic resistance elicitors in cape gooseberry seedlings infected with Fusarium oxysporum f.sp. physali. Plants 9 (2): 176.
Duchanee S. 2015. Identification of the causal fungi of stem and root black rot disease in cassava. Master’s Thesis, School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Thailand.
Gawade B., Sirohi A. 2011. Induction of resistance in eggplant (Solanum melongena) by salicylic acid against root-knot nematode, Meloidogyne incognita. Indian Journal of Nematology 41 (2): 201–205.
Gharib F.A. 2006. Effect of salicylic acid on the growth, metabolic activities and oil content of basil and marjoram. International Journal of Agriculture and Biology 4: 485–492.
Hadi M.R., Balali G.R. 2010. The effect of salicylic acid on the reduction of Rizoctonia solani damage in the tubers of Marfona potato cultivar. Journal of Agricultural and Environmental Sciences 7 (4): 492–496.
Hayat S., Ahmad A. 2007. Salicylic Acid a Plant Hormone. Springer Publishers Dordrecht, The Netherlands.
Hayat Q., Hayat S., Irfana M., Ahmad A. 2010. Effect of exogenous salicylic acid under changing environment: A review. Environmental and Experimental Botany 68: 14–25.
Hinarejos E., Castellano M., Rodrigo I., Belles J.M., Conejero V., Lopez-Gresa M.P., Lison P. 2016. Bacillus subtilis IAB/BS03 as a potential biological control. European Journal of Plant Pathology 146: 597–608.
Jakrawatana N., Pingmuangleka P., Gheewala S.H. 2015. Material flow management and cleaner production of cassava processing for future food, feed and fuel in Thailand. Journal of Cleaner Production 134: 633–641.
Javaheri M., Mashayekhi K., Dadkhah A., Tavallaee F.Z. 2012. Effects of salicylic acid on yield and quality characters of tomato fruit (Lycopersicum esculentum Mill.). International Journal of Agriculture and Crop Sciences 4 (16): 1184–1187.
Jonathan G.S., Diabaté S., Joseph K.K., Odette D.D., Yves-Alain B. 2015. Improvement of cassava resistance to Colletotrichum gloeosporioïdes by salicylic acid, phosphorous acid and fungicide Sumi 8. International Journal of Current Microbiology and Applied Sciences 4 (3): 854–865.
Khandaker L., Masum A.S.M.G., Shinya O.B.A. 2011. Foliar application of salicylic acid improved the growth, yield and leaf ’s bioactive compounds in red amaranthus (Amaranthus tricolor). Vegetable Crops Research Bulletin 74: 77–86.
Kloepper J.W., Ryu C.M., Zhang S. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94: 1259–1266.
Le Thanh T., Thumanu K., Wongkaew S., Boonkerd N., Teaumroong N., Phansak P., Buensanteai N. 2017. Salicylic acidinduced accumulation of biochemical components associated with resistance against Xanthomonas oryzae pv. Oryzae in rice. Journal of Plant Interactions 12 (1): 108–120.
Malandrakis A., Daskalaki E.R., Skiada V., Papadopoulou K.K., Kavroulakis N. 2018. A Fusarium solani endophyte vs fungicides: Compatibility in a Fusarium oxysporum f.sp. radicislycopersici – tomato pathosystem. Fungal Biology 122: 1215–1221.
Narasimhan A., Shivakumar S. 2016. Biocontrol of Rhizoctonia solani root rot of chilli by Bacillus subtilis formulations underpot conditions. Journal of Biological Control 30 (2): 109–118.
Nikaji J., Saengchan C., Wongkeaw S., Buensanteai S., Athinuwat D., Buensanteai N. 2015. Efficacy of bioformulation against Erwinia carotovora pv. carotovora, causal agent of soft rot disease in Chinese cabbage. p. 127–134. In: Proceedings of the 2015 International Forum-Agriculture, Biology and Life Science (IFABL). 23–25 June 2015, Sapporo, Japan.
Onyeka T.J., Ekpo E.J.A., Dixon A.G.O. 2005. Identification of levels of resistance to cassava root rot disease (Botryodiplodia theobromae) in African landraces and improved germplasm using in vitro inoculation method. Euphytica 145: 281–288.
Panuweta P., Siriwongb W., Prapamontolc T., Ryana P.B., Fiedlerd N., Robsone M.G., Barr D.B. 2013. Agricultural pesticide management in Thailand: Situation and population health risk. Environmental Science and Policy 17: 72–81.
Patil S., Sriram S., Savitha M.J. 2011. Evaluation of non-pathogenic Fusarium for antagonistic activity against Fusarium wilt of tomato. Journal of Biological Control 25 (2): 118–123.
Piyachomkwan K., Tanticharoen M. 2011. Cassava industry in Thailand prospects. The Journal of the Royal Institute of Thailand 3: 160–170.
Polthanee A., Janthajam C., Promkhambut A. 2014. Growth, yield and starch content of cassava following rainfed lowland rice in northeast Thailand. International Journal of Agricultural Research 9: 319–324.
Prakongkha I., Sompong M., Wongkaew S., Athinuwat D., Buensanteai N. 2013. Foliar application of systemic acquired resistance (SAR) inducers for controlling grape anthracnose caused by Sphaceloma ampelinum deBary in Thailand. African Journal of Biotechnology 12 (33): 5140–5147.
Prathuangwong S., Buensanteai N. 2007. Bacillus amyloliquefaciens induced systemic resistance against bacterial pustule pathogen with increased phenols peroxides and 1, 3-β-glucanase in soybean plant. Acta Phytopathologica et Entomologica Hungarica 42: 321–330.
Prathuangwong S., Kasem S. 2004. Screening and evaluation of thermotolerant epiphytic bacteria from soybean leaves for controlling bacterial pustule disease. Thai Journal of Agricultural Science 37: 1–8.
Raskin I., Turner I., Melander W.R. 1989. Regulation of heat production in the inflorescences of an Arum lily by endogenous salicylic acid. Proceedings of the National Academy of Sciences 86: 2214–2218.
Romkhambut R. 2015. Effect of stake storage methods on germination, growth and yield of cassava (Manihot esculenta Crantz.). International Journal of Environmental and Rural Development 6 (2): 110–114.
Rozhon W., Petutschnig E., Wrzaczek M., Jonak C. 2005. Quantification of free and total salicylic acid in plants by solidphase extraction and isocratic high-performance anionexchange chromatography. Analytical and Bioanalytical Chemistry 382: 1620–1627.
Ryu C.M., Farag M.A., Hu C.H., Reddy M.S., Kloepper J.W., Pareì P.W. 2004. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiology 134: 1017–1026.
Sangpueak R., Phansak P., Buensanteai N. 2018. Morphological and molecular identification of Colletotrichum species associated with cassava anthracnose in Thailand. Journal of Phytopathology 166: 129–142.
Sompong M., Wongkaew S., Tantasawat P., Buensanteai N. 2012. Morphological pathogenicity and virulence characterization of Sphaceloma ampelinum the causal agent of grape anthracnose in Thailand. African Journal of Microbiology Research 6 (10): 2313–2320.
Song M., Yun H.Y., Kim Y.H. 2014. Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum. Journal of Ginseng Research 38 (2): 136–145.
Sriket S., Thanachit S., Anusontpornperm S. 2015. Effect of fertilizer rates on cassava grown on Yasothon soil amended with cassava stem base biochar and wastes from cassava starch manufacturing plant. Khon Kaen Agriculture Journal 43 (4): 755–762.
Terry E.R., Hahn S.K. 2009. The effect of cassava mosaic disease on growth and yield of a local and an improved variety of cassava. Journal of Pest Management 26: 34–37.
Treesilvattanakul K. 2016. Deterministic factors of Thai cassava prices: multi-uses of cassava from food feed and fuel affecting on Thai cassava price volatility. p. 12–16. In: ICoA Conference Proceedings. 7–9 November, Matsuyama, Japan.
Vallad G.E., Goodman R.M. 2004. Systemic acquired resistance and induced systemic resistance in conventional agriculture. Crop Science 44: 1920–1934.
Wokocha R.C., Nneke N.E., Umechurba C.I. 2010. Screening Colletotrichum gloeospoeioides f.sp. manihotis isolates for virulence on cassava in Akwa Ibom State of Nigeria. Journal of Agriculture, Science and Technology 9: 56–63.
Yildirim E., Guvenc I., Karatas A. 2006. Effect of different number foliar salicylic acid applications on plant growth and yield of cucumber. In: VI National Vegetable Growing Symposium, 19–22 September 2006, Kahramanmaras, Turkey.
Zhang Y., Shi X., Li B., Zhang Q., Liang W., Wang C. 2016. Salicylic acid confers enhanced resistance to Glomerella leaf spot in apple. Plant Physiology and Biochemistry 106: 64–72.