Expression of the pathogenesis related proteins, NH-1, PAL, and lipoxygenase in the iranian Tarom and Khazar rice cultivars, in reaction to Rhizoctonia solani - the causal agent of rice sheath blight
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Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Mazandaran, Sari, P.O. BOX 578, Iran
Mohammad Sayari
Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Mazandaran, Sari, P.O. BOX 578, Iran
Submission date: 2013-03-30
Acceptance date: 2014-01-20
Journal of Plant Protection Research 2014;54(1):36–43
Pathogenesis related (PR) genes of rice are among the most important defense genes in the interaction of rice with pathogens. In this study, the role of NH-1, several PR genes, phenylalanine ammonia-lyase (PAL), and lipoxygenase in the defense responses of rice against Rhizoctonia solani, the causal agent of rice sheath blight disease, was evaluated. The Tarom and Khazar cultivars (cvs), as resistant and susceptible genotypes, respectively, were used. The expression rate of defense genes in two-week-old seedlings inoculated with a virulent isolate of R. solani AG-I-1 A was investigated. The lesions in the Tarom cv were less than half the size of those on the Khazar cv. The expression scripts of the genes were calculated by quantative Real-Time PCR (RT-PCR). Results showed that the expression rate of all genes in the resistant cultivar was higher than that of the susceptible genotype, post inoculation. Analysis of data by the t-Student test also indicated significant differences in the expression level of the genes between Khazar and Tarom. The results of this study suggest that the investigated genes are involved in the resistance responses of rice against the sheath blight agent. For the first time, the induction of PR-5, PR-9, PR-10, PR-12, PR-13, and NH-1 was observed in this study in the resistant and susceptible Iranian cultivars of rice following attacks by R. solani.
The authors have declared that no conflict of interests exist.
Bera S., Purkayastha R.P. 1999. Multicomponent coordinated defence response of rice to Rhizoctonia solani causing sheath blight. Curr. Sci. 76 (34): 1376–1384.
Bouarab K., Brisson N., Daayf F. 2009. Molecular Plant-Microbe Interactions. CAB International, Cambridge, 337 pp.
Broglie K., Chet I., Holliday M., Cressman R., Biddle P., Knowlton S., Mauvais C.J., Broglie R. 1991. Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254 (5035): 1194–1197.
Chern M.S., Fitzgerald H.A., Canlas P.E., Navarre D.A., Ronald P.C. 2005. Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol. Plant-Microbe Interact. 18 (6): 511–520.
Creelman R.A., Mullet J.E. 1997. Biosynthesis and action of jasmonates in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol. 48 (1): 355–381.
Croft K., Juttner F., Slusarenko A.J. 1993. Volatile products of the lipoxygenase pathway evolved from Phaseolus vulgaris (L.) leaves inoculated with Pseudomonas syringae pv. phaseolicola. Plant Physiol. 101 (1): 13–24.
Datta K., Tu J.M., Oliva N., Ona I., Velazhahan R. 2001. Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars. Plant Sci. 160 (3): 405–414.
Datta R.,Velazhahan N., Oliva I., Ona T., Mew GS., Kush S. 1999. Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease. Theor. Appl. Genet. 98 (2): 1138–1145.
Dong H., Beer S.V. 2000. Riboflavin induced disease resistance in plants by activating a novel signal transduction pathway. Phytopathology 90 (8): 801–811.
Edreva A. 2005. Pathogenesis-related proteins: Research progress in the last 15 years. Gen. Appl. Plant Physiol. 31 (1–2): 105–124.
Epple P., Apel K., Bohlmann H. 1997. Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum. Plant Cell 9 (4): 509–520.
Florack D.E.A., Visser B., De Vries Ph.M., Van Vuurde J.W.L., Stiekema W.J. 1993. Analysis of the toxicity of purothionins and hordothionins for plant-pathogenic bacteria. Eur. J. Plant Pathol. 99 (5–6): 259–268.
Gao A.G., Hakimi S.M., Mittanck C.A., Wu Y., Woerner B.M., Stark D.M., Shah D.M., Liang J., Rommens C.M.T. 2000. Fungal pathogen protection in potato by expression of a plant defensin peptide. Nat. Biotechnol. 18 (12): 1307–1310.
Ge X., Song F., Zheng Z. 1999. Systemic acquired resistance to Magnaporthe grisea in rice induced by BTH. Acta Agric. Zhejiangensis 11 (4): 311–314.
Grover A., Gowthaman R. 2003. Strategies for development of fungus-resistant transgenic plants. Curr. Sci. 84 (3): 330–340.
Hamiduzzaman M.M., Jakab G., Barnavon L., Neuhaus J.M., Mauch-Mani B. 2005. Beta-Aminobutyric acidinduced resistance against downy mildew in grapevine acts through the potentiation of callose formation and jasmonic acid signaling. Mol. Plant-Microbe Interact. 18 (8): 819–829.
Hao Z., Wang L., Huang F., Tao R. 2012. Expression of defense genes and antioxidant defense responses in rice resistance to neck blast at the preliminary heading stage and full heading stage. Plant Physiol. Biochem. 57 (2): 222–230.
Hilaire E., Young S.A., Willard L.H., McGee J.D., Sweat T., Chittoor J.M., Guikema J.A., Leach J.E. 2001. Vascular defense responses in rice: Peroxidase accumulation in xylem parenchyma cells and xylem wall thickening. Mol. Plant-Microbe Interact. 14 (12): 1411–1419.
Hon W.C., Griffith M., Mlynarz A., Kwok Y.C., Yang D.S.C. 1995. Antifreeze proteins in winter rye are similar to pathogenesis-related proteins. Plant Physiol. 109 (3): 879–889.
Iwai T., Kaku H., Honkura R., Nakamura S., Ochiai H., Sasaki T., Ohashi Y. 2002. Enhanced resistance to seed-transmitted bacterial diseases in transgenic rice plants over producing an oat cell-wall-bound thionin. Mol. Plant-Microbe Interact. 15 (6): 515–521.
Jaishree M., Chittoor J., Leach E., Frank F. 1997. Differential induction of a peroxidase gene family during infection of rice by Xanthomonas oryzae p v. oryzae. Mol. Plant-Microbe Interact. 10 (7): 861–871.
Jones J.D.G., Dangl J.L. 2006. The plant immune system. Nature 444 (2): 323–329.
Jwa N.S., Agrawal G.K., Rakwal R., Park C.H., Agrawal V.P. 2001. Molecular cloning and characterization of a novel jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10, from rice (Oryza sativa L.) seedling leaves. Biochem. Biophys. Res. Commun. 286: 973–983.
Kagale S., Marimuthu T., Kagale J., Thayumanavan B., Samiyappan R. 2011. Induction of systemic resistance in rice by leaf extracts of Zizyphus jujuba and Ipomoea carnea against Rhizoctonia solani. Plant Signal Behav. 6 (7): 919–923.
Kauffmann S., Legrand M., Geoffroy P. 1987. Biological function of “pathogenesis-related” proteins: Four PR proteins of tobacco have 1,3-β-glucanase activity. EMBO J. 6 (11): 3209–3212.
Kawasaki T., Henmi K., Onstenk J., Hatakeyama S., Iwano M., Satoh H., Shimamoto K. 1999. The small GTP-binding protein Rac is a regulator of cell death in plants. Proc. Natl. Acad. Sci. USA 96 (19): 10922–10926.
Kim S.T., Lim D.S., Canman C.E., Kastan M.B. 1999. Substrate specificities and identification of putative substrates of ATM-kinase family members. J. Biol. Chem. 274 (3): 37538–37543.
Kononowicz A.K., Nelson D.E., Singh N.K., Hasegawa M., Bressan R.A. 1992. Regulation of the osmotin gene promoter. Plant Cell 4 (5): 513–524.
Lay F.T., Anderson M.A. 2005. Defensins-components of the innate immune system in plants. Curr. Protein Pept. Sci. 6 (1): 85–101.
Lin W., Anuratha C.S., Datta K., Potrykus I., Muthukrishnan S., Datta S.K. 1995. Genetic engineering of rice for resistance to sheath blight. Biotechnology 3 (7): 686–691.
Livak K.J., Schmittgen T.D. 2001. Analysis of relative gene expression data using realtime quantitative PCR and the 2ΔΔC (T) method. Methods 25 (4): 402–408.
Malamy J., Carr J.P., Klessig D.F., Raskin I. 1990. Salicylic acid: A likely endogenous signal in the resistance response of tobacco to viral infection. Science 250 (4983): 1002–1004.
Midoh N., Iwata W. 1996. Cloning and characterization of a probenazole-inducible gene for an intracellular pathogenesis-related protein in rice. Plant Cell Physiol. 37 (1): 9–18.
Muthukrishnan S., George H., Liang H., Trick N., Bikram S. 2001. Pathogenesis-related proteins and their genes in cereals. Plant Cell, Tissue and Organ Culture 64 (2–3): 93–114.
Ohta H., Shida K., Peng Y.L., Furusawa I., Shishiyama J., Aibara S., Morita Y. 1991. A lipoxygenase pathway is activated in rice after infection with the rice blast fungus Magnaporthe grisea. Plant Physiol. 97 (1): 94–98.
Olli S., Kirti P.B. 2006. Cloning, characterization and antifungal activity of defensin Tfgd1 from Trigonella foenum-graecum L. J. Biochem. Mol. Biol. 39 (3): 278–283.
Parashina E.V., Serdobinskii L.A., Kalle E.G., Lavorova N.V., Avetisov V.A., Lunin V.G., Naroditskii B.S. 2000. Genetic engineering of oilseed rape and tomato plants expressing a radish defensin gene. Rus. J. Plant Physiol. 47 (3): 417–423.
Passardi F., Penel C., Dunand C. 2004. Performing the paradoxical: How plant peroxidases modify the cell wall. Trends Plant Sci. 9 (11): 534–540.
Rance I., Fournier J., Esquerre-Tugaye M.T. 1998. The incompatible interaction between Phytophthora parasitica var. nicotianae race 0 and tobacco is suppressed in transgenic plants expressing antisense lipoxygenase sequences. Proc. Natl. Acad. Sci. USA. 95 (11): 6554–6559.
Raskin I., Skubatz H., Tang W., Meeuse B.J.D. 1990. Salicylic acid levels in thermogenic and non-thermogenic plants. Ann. Bot. Lond. 66 (4): 369–373.
Rayapuram C.W., Haas C., Baldwin I.T. 2008. PR-13/Thionin but not PR-1 mediates bacterial resistance in Nicotiana attenuata in nature, and neither influences herbivore resistance. Mol. Plant-Microbe Interact. 21 (7): 988–1000.
Schaffrath U., Zabbai F., Dudler R. 2000. Characterization of RCI-1, a chloroplastic rice lipoxygenase whose synthesis is induced by chemical plant resistance activators. Eur. J. Biochem. 267 (19): 5935–5942.
Schweizer D.B., Buchala A., Métraux J.P. 1997. Gene expression patterns and levels of jasmonic acid in rice treated with the resistance inducer 2,6-dichloroisonicotinic acid. Plant Physiol. 115 (1): 61–70.
Sela-Buurlage M.B., Ponstein A.S., Bres-Vloemans S.A., Melchers L.S., Van den Elzen P.J.M., Cornelissen B.J.C. 1993. Only specific tobacco (Nicotiana tabacum) chitinases and β-1,3-glucanases exhibit antifungal activity. Plant Physiol. 101 (2): 857.
Shah Kachroo P., Klessig F.D. 1999. The Arabidopsis ssi1 mutation restores pathogenesis-related gene expression in npr1 plants and renders defensin gene expression salicylic acid dependent. Plant Cell 11 (2): 191–206.
Silverman P., Seskar M., Kanter D., Schweizer P., Metraux J.P., Raskin I. 1995. Salicylic acid in rice (biosynthesis, conjugation, and possible role). Plant Physiol. 108 (2): 633–639.
Somssich I.E., Schmelzer E., Bollmann J., Hahlbrock K. 1986. Rapid activation by fungal elicitor of genes encoding “pathogenesis-related” proteins in cultured parsley cells. Proc. Natl. Acad. Sci. USA 83 (20): 2427–2430.
Taheri P., Tarighi S. 2009. A study on the effect of riboflavin as a defense activator in rice against Rhizoctonia diseases. J. Plant Prot. 23 (1): 68–80.
Terras FR., Eggermont K., Kovaleva V., Raikhel N.V., Osborn R.W., Kester A., Rees S.B., Torrekens S., Van Leuven F., Vanderleyden J. 1995. Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7 (5): 573–588.
Triant D., Whitehead A. 2008. Simultaneous extraction of highquality RNA and DNA from small tissue samples. J. Hered. 100 (2): 246–250.
Van Loon L.C. 1997. Induced resistance in plants and the role of pathogenesis-related proteins. Eur. J. Plant Pathol. 103 (9): 753–765.
Velazhahan R., Chen-Cole K., Anuratha C.S., Muthukrishnan S. 1998. Induction of thaumatin-like proteins (TLPs) in Rhizoctonia solani-infected rice and characterization of two new cDNA clones. Physiol. Plant 102 (1): 21–28.
Wasternack C., Atzorn R., Jarosch B., Kogel K.H. 1994. Induction of a thionin, the jasmonate-induced 6-kDa protein of barley by 2,6-dichloro-isonicotinic acid. J. Phytopathol. 140 (2): 280–284.
Zhang W., Ge X., Song F., Zheng Z. 2004. Systemic acquired resistance of rice against sheath blight disease induced by benzothiadiazole. Acta Phytophylacica Sin. 30 (2): 171–176.
Zhao C.G., Wang A.R., Shi Y.J., Wang L.Q., Liu W.D., Wang Z.H., Lu G.D. 2008. Identification of defense-related genes in rice responding to challenge by Rhizoctonia solani. Theor. Appl. Genet. 116 (4): 501–516.
Zhu B., Chen T.H.H., Li P.H. 1995. Activation of two osmotinlike protein genes by abiotic stimuli and fungal pathogen in transgenic potato plants. Plant Physiol. 108 (3): 929–937.