ORIGINAL ARTICLE
First record of a novel begomovirus and satellites associated with leaf curl disease of passion fruit from India
V Venkataravanappa 1, A-D  
,   C N Lakshminarayana Reddy 2, C,E,   Shridhar Hiremath 2, C,   B M Muralidhara 3, C,E,   V. Suryanarayana 4, E,   V K. Baranwal 5, B,E,   M. Krishna Reddy 6, A,D
 
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1
Division of Plant Pathology, Division of Plant Pathology Central Horticultural Experiment Station, Chettalli, ICAR-Indian Institute of Horticultural Research, Madikeri District Karnataka, India PIN Code:571 248, India
2
Department of Plant Pathology,, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore-560065, Karnataka, India Email: cnlreddy@gmail.com, India
3
Dept. of Pomology, ICAR-Directorate of Cashew Research, Puttur-574202, Karnataka, India, India
4
Department of Forest Biology and Tree Improvement,, College of Forestry, UAS-Dharwad, Sirsi-581401, Karnataka, India, India
5
Division of Plant Pathology, , ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India, India
6
Division of Crop protection, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore-560089, Karnataka, India, India
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
V Venkataravanappa   

Division of Plant Pathology, Division of Plant Pathology Central Horticultural Experiment Station, Chettalli, ICAR-Indian Institute of Horticultural Research, Madikeri District Karnataka, India PIN Code:571 248, Central Horticultural Experiment Station (CHES)-Ch, 571 248, Madikeri, India
Submission date: 2021-08-11
Acceptance date: 2021-11-19
Online publication date: 2021-12-13
 
 
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ABSTRACT
Passion fruit is an important fruit crop grown in parts of southern and north-eastern states of India. Leaf curl symptoms typical to begomovirus infection were observed on passion fruit plants at three locations of Madikeri District, Karnataka State, India. The disease incidence ranged from 10-20% in all the locations. In order to determine if the begomovirus was associated with leaf curl disease of passion fruit, 20 infected samples collected from different locations were subjected to PCR analysis using primers specific to begomovirus. This resulted in an expected PCR product of ~ 1.2 kb. Sequence analysis of these products revealed that they have more than 98% similarity among them and have similarity with other begomoviruses. Complete genome sequencing of begomovirus associated with one sample (PF1 collected from CHES, Madikeri) was done using RCA. Further, sequencing of betasatellite and alphasatellite was done after PCR amplification using specific primers. Complete DNA-A sequence of PF-isolate with other begomoviruses revealed that it shared nucleotide (nt) identity of 87.8 to 88.8% with Ageratum enation virus. This indicated the association of a novel begomovirus with leaf curl disease of passion fruit in India, for which we propose the name, Passion fruit leaf curl virus (PFLCuV) [IN-Kar-18]. PFLCuV associated betasatellite shared 98.3% sequence identity with Tomato leaf curl Bangladesh betasatellite, while alphasatellite had 95.7% sequence identity with Cotton leaf curl Multan alphasatellite. Recombinant analysis indicated a major component of PFLCuV DNA-A may have originated from a recombination of earlier reported begomoviruses. Recombination as well as GC plot analysis showed that the recombination occurred in the genome regions having low GC content regions of PFLCuV. However, there is no evidence of recombination in alphasatellite and betasatellite associated with leaf curl disease of passion fruit. This is the first record of a novel begomovirus and satellites associated with leaf curl disease of passion fruit from India.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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