First report of leaf spot disease of Aloe vera caused by Fusarium proliferatum in India
Shubhi Avasthi 1, 2, 3, A-F  
Ajay Kumar Gautam 2, C-E
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School of Studies in Botany, Jiwaji University, Gwalior, Madhya Pradesh, India
School of Agriculture, Abhilashi University, Mandi, Himachal Pradesh, India
Department of Botany, Abhilashi Institute of Post Graduate Studies, Mandi, Himachal Pradesh, India
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2017-10-17
Acceptance date: 2017-12-18
Journal of Plant Protection Research 2018;58(2):109–114
Severe leaf spot disease was observed on Aloe vera plants in the winters of 2011 and 2012 during the survey of various nurseries of Gwalior, India. Irregular, sunken, dark creamish brown spots having reddish brown margin were noticed on both the surface of leaves. The causal organism consistently isolated from symptomatic leaves on potato dextrose agar media. Total 59 isolates of fungi recovered from diseased A. vera leaves, 37 isolates were identified as belonging to the genus Fusarium. On the basis of morphological characteristic and internal transcribed spacer (ITS) region of rDNA amplified using the primers ITS4/ITS5 the pathogen was identified as Fusarium proliferatum (Matsushima) Nirenberg and pathogenicity of the isolate was confirmed by using Koch’s postulates. To the best of our knowledge, this is the first report of leaf spot disease caused by F. proliferatum on A. vera plant in India.
The authors have declared that no conflict of interests exist.
Shubhi Avasthi   
School of Studies in Botany, Jiwaji University, Gwalior, Madhya Pradesh-474011, India
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