ORIGINAL ARTICLE
Formulation of the endophytic fungus Cladosporium oxysporum Berk. & M.A. Curtis, isolated from Euphorbia bupleuroides subsp. luteola, as a new biocontrol tool against the black bean aphid (Aphis fabae Scop.)
 
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1
Laboratory of Improvement of the Phytosanitary Protection Techniques in Mountainous Agrosystems, Agronomy Department, ISVSA, University Hadj Lakhdar, Batna 05000, Algeria
2
Laboratory of Applied Microbiology, Faculty of Natural Sciences and Life, University Ferhat Abbas Sétif 1, 19000, Algeria
CORRESPONDING AUTHOR
Oussama Ali Bensaci
Laboratory of Improvement of the Phytosanitary Protection Techniques in Mountainous Agrosystems, Agronomy Department, ISVSA, University Hadj Lakhdar, Batna 05000, Algeria
Submission date: 2014-07-16
Acceptance date: 2015-02-16
 
Journal of Plant Protection Research 2015;55(1):80–87
KEYWORDS
TOPICS
ABSTRACT
Two formulations containing culture filtrates and conidial suspensions of the endophytic fungus Cladosporium oxysporum Berk. & M.A. Curtis, isolated previously from stems of Euphorbia bupleuroides subsp. luteola (Kralik) Maire, were experimentally tested for their aphicid activity against the black bean aphid Aphis fabae Scop. found in Algeria. It was shown that invert emulsions are more effective against aphids, than using aqueous suspensions. This was especially true for formulations containing culture filtrates. The relatively insignificant mortalities obtained by formulations containing conidial suspensions indicated a low infectious potential towards the aphids. The proteolytic activity seemed to be more important than the chitinolytic activity of the fungus against the black bean aphid A. fabae.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (49)
1.
Abbott W.S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18 (2): 265–267.
 
2.
Abdel-Baky N.F., Abdel-Salam A.H. 2003. Natural incidence of Cladosporium spp. as a bio-control agent against whiteflies and aphids in Egypt. Journal of Applied Entomology 127 (4): 228–235.
 
3.
Aldeghairi M.A., El-Meleigi M.A., Abdel Baky N.F., Ibrahim G.H. 2013. The entomopathogenic fungus Cladosporium sp. as a candidate biocontrol agent against the sweetpotato whitefly, Bemisia Tabaci in Saudi Arabia. p. 532–563. In: “Recent Advances in Biofertilizers and Biofungicides (PGPR) for Sustainable Agriculture”. Proceedings of 3rd Asian Conference on Plant Growth-Promoting Rhizobacteria (PGPR) and other Microbials. Manila, Philippines, 21–24 April 2013, 568 pp.
 
4.
Assaf L.H. 2009. The efficiency of Beauveria bassiana (Bals.) Vuill. and Paecilomyces farinosus(Dicks ex Fr.) for biological control of bean aphid Apis fabae Scopoli. p. 14–20. In: Proceedings of the 4th Conference on Recent Technologies in Agriculture. Cairo, Egypt, 3–5 November 2009.
 
5.
Auld B.A., McRae C. 1997. Emerging technologies in plant protection – bioherbicides. p. 191–194. In: Proceedings of the 50th NZ Plant Protection Conference, New Zealand, 1997, 555 pp. http://www.nzpps.org/nzpp_cont....
 
6.
Batta Y.A. 2003. Effect of treatment with Trichoderma Harzianum Rifai formulated in invert emulsion on postharvest decay of apple blue mold. International Journal of Food Microbiology 96 (3): 281–288.
 
7.
Batta Y.A. 2004. Postharvest biological control of apple grey mold by Trichoderma harzianum Rifai formulated in an invert emulsion. Crop Protection 23 (1): 19–26.
 
8.
Batta Y.A. 2007. Biocontrol of almond bark beetle (Scolytus amygdali Geurin-Meneville, Coleoptera: Scolytidae) using Beauveria bassiana(Bals.) Vuill. (Deuteromycotina: Hyphomycetes). Journal of Applied Microbiology 103 (5): 1406–1414.
 
9.
Bensaci O.A., Lombarkia N., Rouabah K. 2012. Endophytic fungi from Echinus molle L. as new biological control agents of black bean aphid in Algeria. p.100. In: “IPM on the World Stage”. 7th International Integrated Pest Management Symposium. Memphis, Tennessee, USA, 27–29 March 2012, 150 pp.
 
10.
Bensaci O.A., Lombarkia N., Laib D.E. 2013. Initial evaluation of endophytic fungi, isolated from Nerium Oleander L. for their biocontrol action against the bruchid Acanthoscelides obtectus(Say) (Coleoptera: Bruchidae) in Algeria. p. 162–167. In: “Endophytes for Plant Protection: the State of the Art”. Proceedings of the 5th International Symposium on Plant Protection and Plant Health in Europe. Humboldt University Berlin, Berlin-Dahlem, Germany, 26–29 May 2013, 333 pp.
 
11.
Bond D.A., Lowe H.J.B. 1975. Tests for resistance to Aphis fabae in field beans (Vicia faba). Annals of Applied Biology 81 (1): 21–32.
 
12.
Butt T.M., Goettel M.S. 2000. Bioassays of entomogenous fungi. p. 141–195. In: “Bioassays of Entomopathogenic Microbes and Nematodes” (A. Navon, K.R.S. Ascher, eds.). CAB International, Wallingford, UK, 324 pp.
 
13.
Cammell M.E. 1981. The black bean aphid, Aphis fabae. Biologist 28: 247–258.
 
14.
Cammell M.E., Tatchell G.M., Woiwod I.P. 1989. Spatial pattern of abundance of the black bean aphid, Aphis fabae, in Britain. Journal of Applied Ecology 26 (2): 463–472.
 
15.
Connick W.J., Daigle D.J., Quimby P.C. 1991. An improved invert emulsion with high water retention for mycoherbicide delivery. Weed Technology 5 (2): 442–444.
 
16.
Crous P.W., Braun U., Schubert K., Groenewald J.Z. 2007. Delimiting Cladosporium from morphologically similar genera. Studies in Mycology 58 (1): 33–56.
 
17.
Dedryver C.A. 1979. Déclenchement en serre d’une épizootie a Entomophthora fresenii sur Apis Fabae par introduction d’un inoculum et régulation de l’humidité relative. [Initiaiting an epizootic in a glasshouse with Enthomophtora fresenii on Aphis fabae through inoculum introduction and control of the relative humidity]. Entomophaga 24 (4): 443–453. (in French with English summary).
 
18.
Dorschner K.W., Feng M., Baird C.R. 1991. Virulence of an aphidderived isolate of Beauveria bassiana (Fungi: Hyphomycetes) to the hop aphid, Phorodon humuli(Homoptera: Aphididae). Environmental Entomology 20 (2): 690–693.
 
19.
Franz A., Katul L., Makkouk K.M., Vetten H.J. 1996. Monoclonal antibodies for the detection and differentiation of faba bean necrotic yellows virus isolates. Annals of Applied Biology 128 (2): 255–268.
 
20.
Franz A.W., van der Wilk F., Verbeek M., Dullemans A.M., van den Heuvel J.F. 1999. Faba bean necrotic yellows virus (genus Nanovirus) requires a helper factor for its aphid transmission. Virology 262 (1): 210–219.
 
21.
Giove R.M., Abis S. 2007. Place de la Méditerranée dans la production mondiale de fruits et légumes. [Place of the Mediterranean in the world production of fruits and vegetables]. Les notes d’analyse du CIHEAM 23: 1–21. (in French).
 
22.
Guesmi-Jouini J., Boughalleb-M ́hamdi N., Ben Halima-Kamel M. 2010. Etudes préliminaires sur les champignons entomopathogènes des pucerons de l’artichaut en Tunisie. [Preliminary studies on entomopathogenic fungi of artichoke aphids in Tunisia]. Entomologie Faunistique – Faunistic Entomology 63 (3): 171–181. (in French).
 
23.
Halimona J., Jankevica L. 2011. The influence of entomophthorales isolates on aphids Apis fabae and Metopeurum fuscoviride. Latvijas Entomologs 50: 55–60.
 
24.
Hassan A.H.A. 2007. Novel natural products from endophytic fungi of Egyptian medicinal plants – chemical and biological characterization. Doctoral thesis, University of Düsseldorf, Germany, 270 pp.
 
25.
Heuchert B., Braun U., Schubert K. 2005. Morphotaxonomic revision of fungicolous Cladosporium species (hyphomycetes). Schlechtendalia 13: 1–78.
 
26.
Ho M.H.M., Castañeda R.F., Dugan F.M., Jong S.C. 1999. Cladosporium and Cladophialophora in culture: descriptions and an expanded key. Mycotaxon 72: 115–157.
 
27.
Jeyarani S., Banu J.G., Ramaraju K. 2011. First record of natural occurrence of Cladosporium cladosporioides(Fresenius) de Vries and Beauveria bassiana(Bals.-Criv.) Vuill on two spotted spider mite, Tetranychus urticae Koch from India. Journal of Entomology 8 (3): 274–279.
 
28.
Kucera M. 1980. Proteases from the fungus Metarhizium Anisopliae toxic for Galeria mellonella larvae. Journal of Invertebrate Pathology 35 (3): 304–310.
 
29.
Kuk J.H., Jung W.J., Jo G.H., Kim Y.C., Kim K.Y., Park R.D. 2005. Production of N-acetyl-beta-D-glucosamine from chitin by Aeromonas sp. GJ-18 crude enzyme. Applied of Microbiological Biotechnology 68 (3): 384–389.
 
30.
Lopez-Llorca L.V., Olivares-Bernabeu C.M., Salinas J., Jansson H.-B., Kolattukudy P.E. 2002. Pre-penetration events in fungal parasitism of nematode eggs. Mycological Research 106 (4): 499–506.
 
31.
Maatougui M.E.H. 1996. Situation de la culture des fèves en Algérie et perspectives de relance. [The situation of bean crops in Algeria and the revival prospects]. Céréaliculture 29: 6–14. (in French)McKemy J.M., Morgan-Jones G. 1990. Studies in the genus Cladosporium sensu lato. II. Concerning Heterosporium gracile, the causal organism of leaf spot disease of Iris species. Mycotaxon 39: 425–440.
 
32.
Meradsi F. 2009. Contribution à l’étude de la résistance naturelle de la fève Vicia faba L. au puceron noir Apis fabae Scopoli, 1763 (Homoptera: Aphididae). [Contribution to the study of natural resistance faba bean Vicia faba L. against the black aphid Aphis fabae Scopoli, 1763 (Homoptera: Aphididae)]. Master degree thesis. University Hadj Lakhdar Batna, Algeria, 73 pp. (in French).
 
33.
Mesquita A.L.M., Lacey L.A., Mercadier G., LeClant F. 1996. Entomopathogenic activity of a whitefly-derived isolate of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the Russian wheat aphid, Diuraphisnoxia(Hemiptera: Sternorrhyncha Aphididae) with the description of an effective bioassay method. European Journal of Entomology 93 (1): 69–75.
 
34.
Monod M., Capoccia S., Lechenne B., Zaugg C., Holdom M., Jousson O. 2002. Secreted proteases from pathogenic fungi. International Journal of Medical Microbiology 292 (5–6): 405–419.
 
35.
Moscoso I.L., Rosato Y.B. 1987. Extracellular enzyme production by haploids, heterocaryons and diploids of Aspergillus nidulans. Applied Microbiology and Biotechnology 26 (4): 365–368.
 
36.
Nguyen V.N., Oh I.J., Kim Y.J., Kim K.Y., Kim Y.C., Park R.D. 2009. Purification and characterization of chitinases from Paecilomces variotii DG-3 parasitizing on Meloidogyne incognito eggs. Journal of Industrial Microbiology and Biotechnology 36 (2): 195–203.
 
37.
Petch T. 1931. Notes on entomogenous fungi. Transaction of the British Mycological Society 16 (1): 55–75.
 
38.
Rezende S.R.F., Curvello F.A., Fraga M.E., Reis R.C.S., Castilho A.M.C., Agostinho T.S.P. 2009. Control of the Alphitobius diaperinus(Panzer) (Coleoptera: Tenebrionidae) with entomopathogenic fungi. Revista Brasileira de Ciencia Avicola 11 (2): 121–127.
 
39.
Robert A., Messing-Al-Aidroos K. 1985. Acid production by Metarhizium anisopliae: Effect on virulence against mosquitoes and on detection of in vitroamylase, protease, and lipase activity. Journal of Invertebrate Pathology 45 (1): 9–15.
 
40.
Rojas T., Pons N., Arnal E. 1998. Cladosporium herbarium on whiteflies (Homoptera: Aleyrodidae) in Venezuela. Boletin de Entomologia Venezuela, Serie Monografias 13: 57–65.
 
41.
Sabuquillo P., De Cal A., Melgarejo P. 2010. Development of a dried Penicillium oxalicum conidial formulation for use as a biological agent against Fusarium wilt of tomato: Selection of optimal additives and storage conditions for maintaining conidial viability. Biological Control 54 (3): 221–229.
 
42.
Samuels R.I., Paterson I.C. 1995. Cuticle degrading proteases from insect moulting fluid and culture filtrates of entomopathogenic fungi. Comparative Biochemistry Physiology B 110 (4): 661–669.
 
43.
Samways M.J., Grech N.M. 1986. Assessment of the fungus Cladosporium oxysporum(Berk. and Curt.) as a potential biocontrol agent against certain Homoptera. Agriculture, Ecosystem and Environment 15 (4): 231–239.
 
44.
Saranya S., Ushakumari R., Sosamna J., Babu M.P. 2010. Efficacy of different entomopathogenic fungi against cowpea aphid, Aphis craccivora(Koch). Journal of Biopesticides 3 (1): 138–142.
 
45.
Shah P.A., Clark S.J., Pell J.K. 2004. Assessment of aphid host range and isolate variability in Pandora neoaphidis(Zygomycetes: Entomophthorales). Biological Control 29 (1): 90–99.
 
46.
Vega F.E., Posada F., Aime M.C., Pava-Ripoll M., Infante F., Rehner S.A. 2008. Entomopathogenic fungal endophytes. Biological Control 46 (1): 72–82.
 
47.
Vetten H.J. 2010. Nanoviruses. p. 226–233. In: “Desk Encyclopedia of Plant and Fungal Virology” (B.W.J. Mahy, M.H.V. van Regenmortel, eds.). Elsevier, UK, 613 pp.
 
48.
Wilding N., Mardell S.K., Brobyn P.J. 1986. Introducing Erynia neoaphidis into a field population of Aphis fabae: form of the inoculum and effect of irrigation. Annals of Applied Biology 108 (2): 373–385.
 
49.
Yeo H., Pell J.K., Alderson P.G., Clark S.J., Pye B.J. 2003. Laboratory evaluation of temperature effects on the germination and growth of entomopathogenic fungi and on their pathogenicity to two aphid species. Pest Management Science 59 (2): 156–165.
 
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