ORIGINAL ARTICLE
Fulfillment of Koch's postulates for in vitro pathogenicity of Musicillium theobromae (Turconi) Zare & W. Gams as the cause of banana cigar end rot disease
1 | Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, 7616914111 Iran |
2 | Young Researchers Club, Kerman Branch, Islamic Azad University, Kerman, 7619657633 Iran |
CORRESPONDING AUTHOR
Shirin Masudi
Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, 7616914111 Iran Young Researchers Club, Kerman Branch, Islamic Azad University, Kerman, 7619657633 Iran
Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, 7616914111 Iran Young Researchers Club, Kerman Branch, Islamic Azad University, Kerman, 7619657633 Iran
Submission date: 2012-05-17
Acceptance date: 2012-08-20
Journal of Plant Protection Research 2012;52(4):410–414
KEYWORDS
TOPICS
ABSTRACT
Pathogenicity of
Musicillium theobromae
(Turconi) Zare & W. Gams (syn.
Stachylidium theobromae
Turconi,
Verticillium
theo-
bromae
E.W. Mason & S. Hughes) causing Banana cigar end rot disease has fulfilled
Koch’
s Postulates for the first time
. To assess and
prove the pathogenicity,
M. theobromae
mycelial discs
and conidia were
inoculated in triplicate to surface-disinfested green
banana
fruits. The bananas were infected both through artificial wounds
as well as through intact unwounded fruits. After inoculation, the
bananas were covered with moist cotton and incubated for 30
days at 23°C. Plain moist cotton was used on
the controls. Those inocu-
lated treatments kept under high relative humidity
exhibited symptoms of banana cigar end rot disease. The controls
and those kept
under low relative humidity did not develop symptoms and remained healthy. The results indicated that both optimum temperature
and high relative humidity are necessary for infection onset
and disease development. It was also determined that infection can occur
both through wounds and intact unwounded fruit epidermis. The
cultural characteristics in
M. theobromae
were also investigated. It
was
found that the best mycelial growth occurred on potato dextrose agar (PDA) and the optimum pH for mycelial growth was 8.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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