• The present work aimed to find new bio-insecticide to protect wheat stored against insect pests during the long storage months
  • Terpinen-4-ol and γ-Terpinene were detected as major components of M. alternifolia EO
  • The insecticidal activity of the M. alternifolia EO, Terpinen-4-ol and γ-Terpinene was evaluated against S. oryzae
The fumigation toxicity of Melaleuca alternifolia (Maid. & Betche) Cheel. (Myrtales: Myrtaceae) essential oil and its major fractions was studied under laboratory conditions against adults of Sitophilus oryzae L. (Coleoptera: Curculionidae) to protect wheat grains (Triticum aestivum L.) (Poales: Poaceae) from this global pest that destroys the host plant during storage. By analyzing M. alternifolia essential oil (EO) using GC/MS terpinen-4-ol and γ-terpinene were detected as major components. In the fumigation toxicity, M. alternifolia EO showed the highest toxicity (LC50 = 0.31 μl · l–1 air), followed by terpinen-4-ol (LC50 = 23.65 μl · l–1 air) and γ-terpinene was the least toxic (LC50 = 43.55 μl · l–1 air). When tested for their insecticidal activities against S. oryzae in stored wheat, no progeny emerged after 3 months of treatment with M. alternifolia EO at 10 mg · g–1 or with terpinen-4-ol and γ-terpinene for 2 months. However, none of these compounds could protect wheat grain from damage throughout the entire study period (4 months). Interestingly, all tested compounds at the highest application rate did not show any phytotoxic effects after 4 months of storage.
Tomasz Klejdysz
The authors have declared that no conflict of interests exist.
Affognon H., Mutungi C., Sanginga P., Borgemeister C. 2015. Unpacking postharvest losses in sub-Saharan Africa: a meta-analysis. World Devel 66: 49–68.
Campolo O., Giunti G., Russo A., Palmeri V., Zappalà L. 2018. Natural strategies to improve quality in food protection. Journal of Food Quality 2018: 18. DOI: https://doi.org/10.1155/2018/6....
Fang F., Candy K., Melloul E. 2016. In vitro activity of ten essential oils against Sarcoptes scabiei. Parasites and Vectors 9: 594. DOI: https://doi.org/10.1186/s13071....
Finney J.D. 1971. Probit Analysis. 3rd ed. Cambridge University Press, Cambridge, UK, 318 pp.
Hodges R.J., Buzby J.C., Bennett B. 2011. Postharvest losses and waste in developed and less developed countries: Opportunities to improve resource use. Journal of Agricultural Science 149: 37–45. DOI: https://doi.org/10.1017/S00218....
Ibáñez M.D., Blázquez M.A. 2019. Tea tree and wintergreen essential oils in the management of the invasive species Cortaderia selloana and Nicotiana glauca. Journal of Plant Protection Research 59 (2): 160–169. DOI: https://doi.org/10.24425/jppr.....
Ismail S.M., Sleem F. 2021a. Contact toxicity, repellent activity and residual effect of Syzygium aromaticum L. (Myrtaceae) and Piper nigrum L. (Pipeaceae) powders against Sitophilus oryzae L. (Coleoptera: Curculionidae). Journal of Entomology and Zoology Studies 8 (6): 2053–2058.
Ismail S.M., Sleem F. 2021b. Toxicity and residual effect of Annona squamosal L. and Piper nigrum L. seeds extracts against Tribolium castaneum and Sitophilus oryzae. International Journal of Science and Research 10 (12): 517–523. DOI: https://doi.org/10.21275/MR211....
Jagadeesan R., Singarayan V.T., Chandra K., Ebert P.R., Manoj K., Nayak M.K. 2018. Potential of Co-fumigation with phosphine (PH3) and sulfuryl fluoride (SO2F2) for the management of strongly phosphine-resistant insect pests of stored grain. Journal of Economic Entomology 111 (6): 2956–2965.
Jankowska M., Rogalska J., Wyszkowska J., Stankiewicz M. 2018. Molecular targets for components of essential oils in the insect vervous system – a review. Molecules 23 (1): 34. DOI: https://doi.org/10.3390/molecu....
Liao M., Xiao J.J., Zhou L.J., Yao X., Tang F., Hua R.M., Wu X.W., Cao H.Q. 2017. Chemical composition, insecticidal and biochemical effects of Melaleuca alternifolia essential oil on the Helicoverpa armigera. Journal of Applied Entomology 141 (9): 721–728. DOI: https://doi.org/10.1111/jen.12....
Savary S., Ficke A., Aubertot J.N., Hollierm C. 2012. Crop losses due to diseases and their implications for global food production losses and food security. Food Security 4: 519–537. DOI: https://doi.org/10.1007/s12571....
Singh K.D., Mobolade A.J., Bharali R., Sahoo D., Rajashekar Y. 2021. Main plant volatiles as stored grain pest management approach: a review. Journal of Agriculture and Food Research 4: 100127. DOI: https://doi.org/10.1016/j.jafr....
Yadav E., Kumar S., Mahant S., Khatkar S., Rao R. 2016. Tea tree oil: a promising essential oil. Journal of Essential Oil Research 29 (3): 201–213. DOI: https://doi.org/10.1080/104129....
Yang Y., Isman M.B., Tak J.H. 2020. Insecticidal activity of 28 assential oils and a commercial product containing Cinnamomum cassia Bark essential oil against Sitophilus zeamais Motschulsky. Insects 11 (8): 474. DOI: https://doi.org/10.3390/insect....
Yaseen M., Kausar T., Praween B., Shah S.J., Jan Y., Shekhawat S.S., Malik M., Azaz A.A.Z.R. 2019. Insect pest infestation during storage of cereal grains, pulses and oilseeds. p. 209–234. In: “Health and Safety Aspects of Food Processing Technologies” (A. Malik, Z. Erginkaya, H. Erten, eds.). Springer, Cham. DOI: https://doi.org/10.1007/978-3-....