ORIGINAL ARTICLE
Genetic diversity in population of Fusarium solani from cumin in Iran
 
More details
Hide details
1
Department of Plant Pathology, Faculty of Agriculture, Birjand University, Birjand, Iran
2
Faculty of Agriculture, Islamic Azad University of Birjand, Birjand, Iran
 
Journal of Plant Protection Research 2009;49(3):283–286
KEYWORDS
TOPICS
ABSTRACT
Nineteen isolates of Fusarium solani were recovered from crown and root rotted parts of cumin plants collected from the major cumin producing area in Iran during 1999–2000 using Nash-Snyder media. One hundred and fifty eight nit mutants of F. solani were generated using PDA amended with 3% and 5% potassium chlorate. Ofthe nit mutants generated, 47/7%, 26/6% and 25/9% were nit1, nit3 and nitM, respectively. Nit mutants were used to force heterokaryon formation to determine VCGs and their relation to pathogenecity and geographic origin. Fifteen VCGs were determined for F. solani isolates, that 12 were single members VCGs. There was no specific relation between VCGs and geographic origin in F. solani isolates. This is the first research on the genetic diversity of F. solani from cumin.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (18)
1.
Aruna K., Sivaramakrishnan V.M. 1996. Anticarcinogenic effects of the essential oil from cumin, poppy and basil. Phytotherapy Res. 10: 577–580.
 
2.
Booth C. 1971. The Genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, England, 237 pp.
 
3.
Burgess L.W., Summerell B.A., Bullock S., Gott K.P., Back-house D. 1994. Laboratory for Fusarium Research, 3rd ed. Edition, Fusarium Research Laboratory, Department of Crop Sciences, University of Sydney and Royal Botanic Garden, Sydney, 133 pp.
 
4.
Correll J.C., Klittich C.J.R., Leslie J.F. 1987. Nitrate non-utilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology 77: 1640–1646.
 
5.
Dang S.R.S. 1995. Diseases of cumin (Cuminum cyminum L.) and their management. J. Spices Aromatic Crops 4: 57–60.
 
6.
Hawthorne B.T., Rees-George J. 1996. Use of nitrate non-utilizing mutants to study vegetative incompatibility in Fusarium solani(Nectria haematococca) especially members of mating populations. I, V and VI. Mycol. Res. 100: 1075–1081.
 
7.
Jacobson D.J., Gordon T.R. 1988.Vegetative compatibility and self incompatibility within Fusarium oxysporum f. sp. melonis. Phytopathology 78: 668–672.
 
8.
Katan T. 1999. Current status of vegetative compatibility groups in Fusarium oxysporum. Phytoparasitica 27: 51–64.
 
9.
Katan T., Berliner R., Katan J. 1994. Vegetative compatibility in populations of Fusarium oxysporum from wild carnation. Mycol. Res. 98: 1415–1418.
 
10.
Kistler H.C., Alabouvette C., Baayen R.P., Bentley S., Brayford D., Coddington A., Correll J., Daboussi M.J., Elias K., Fernandez D., Gordon T.R., Katan T., Kim H.G., Leslie J.F., Martyn R.D., Migheli Q., Moore N.Y., O’Donnell K., Ploetz R.C., Rutherford M.A., Summerell B., Waalwijk C., Woo S. 1998. Systematic numbering of vegetative compatibility groups in the plant pathogenic fungus Fusarium oxysporum. Phytopathology 88: 30–32.
 
11.
Klittich C.J.R., Leslie J.F. 1988. Nitrate reduction mutants of Fusarium moniliforme (Gibberella fujikuroi). Genetics 188: 417–423.
 
12.
Leslie J.F., Summerell B.A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing Ltd, UK, 387 pp.
 
13.
Leslie J.F. 1993. Fungal vegetative compatibility. Annu. Rev. Phytopathol. 31: 127–150.
 
14.
Moon J.H., Lee Y.H., Lee Y.W. 1999. Vegetative compatibility groups in Fusarium graminearum isolates from corn and barley in Korea. Plant Pathol. J. 15: 53–56.
 
15.
Nooras Mofrad N., Farrokhi Nejad R., Alizadeh A. 2005. Genetic diversity in populations of Fusarium oxysporum f. sp. cumini, the causal agent of cumin wilts in Khorasan using vegetative compatibility groups. Iranian J. Plant Pathol. 41 (3): 437– 453.
 
16.
Peter K.V., Nybe E.V. 2002. Dominating global markets. The Hindu Survey of Indian Agricul.: 87–99.
 
17.
Puhalla J.E. 1985. Classification of strains of Fusarium oxysporum on the basis of vegetative compatibility. Can. J. Bot. 63: 179–183.
 
18.
Sharifi K., Zare R., Rees-George J. 2008. Vegetative compatibility groups among Fusarium solani isolates causing potato dry rot. J. Biolog. Sci. 8: 374–379.
 
eISSN:1899-007X
ISSN:1427-4345