ORIGINAL ARTICLE
Genetic diversity study of Fusarium culmorum: causal agent of wheat crown rot in Iraq
Oadi Matny 1, A,D,F  
,  
Sattar Shamsallah 1, B
,  
Maadh Al Fahad 2, C
,  
 
 
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1
Department of Plant Protection, University of Baghdad, Baghdad, Iraq
2
Department of Plant Protection, University of Tikrit, Tikrit, Iraq
3
Department of Agronomy and Plant Genetics, University of Minnesota, Minnesota, USA
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Oadi Matny   

Department of Plant Protection, University of Baghdad, Baghdad, Iraq
Online publish date: 2019-06-12
Submission date: 2018-10-17
Acceptance date: 2019-05-16
 
KEYWORDS
TOPICS
ABSTRACT
Fusarium crown rot (FCR), caused by Fusarium culmorum (Wm.G.Sm) Sacc., is an important disease of wheat both in Iraq and other regions of wheat production worldwide. Changes in environmental conditions and cultural practices such as crop rotation generate stress on pathogen populations leading to the evolution of new strains that can tolerate more stressful environments. This study aimed to investigate the genetic diversity among isolates of F. culmorum in Iraq. Twenty-nine samples were collected from different regions of wheat cultivation in Iraq to investigate the pathogenicity and genetic diversity of F. culmorum using the repetitive extragenic palindromic (REP-PCR) technique. Among the 29 isolates of F. culmorum examined for pathogenicity, 96% were pathogenic to wheat at the seedling stage. The most aggressive isolate, from Baghdad, was IF 0021 at 0.890 on the FCR severity index. Three primer sets were used to assess the genotypic diversity via REP, ERIC and BOX elements. The amplicon sizes ranged from 200–800 bp for BOX-ERIC2, 110–1100 bp for ERIC-ERIC2 and 200–1300 bp for REP. A total of 410 markers were polymorphic, including 106 for BOX, 175 for ERIC and 129 for the REP. Genetic similarity was calculated by comparing markers according to minimum variance (Squared Euclidean). Clustering analysis generated two major groups, group 1 with two subgroups 1a and 1b with 5 and 12 isolates, respectively, and group 2 with two subgroups 2a and 2b with 3 and 9 isolates, respectively. This is the first study in this field that has been reported in Iraq.
ACKNOWLEDGEMENTS
This work was carried out and supported by the University of Minnesota, Department of Plant Pathology. We wish to thank Dr. Brian Steffenson, Dr. Scott Bates and Dr. Zewei Song for their support and help in completing this paper.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (45)
1.
Albayrak G., Emre Y., Aylin G., Bahram S. 2016. Genetic diversity among Fusarium graminearum and F. culmorum isolates based on ISSR markers. Archives of Biological Sciences 68 (2): 333–343. DOI:10.2298/ABS1506300 25A.
 
2.
Balmas V., Delogu G., Sposito S., Rau M. 2006. Use of a complexation of tebuconazole with b-cyclodextrin for controlling foot and crown rot of durum wheat incited by Fusarium culmorum. The Journal of Agricultural and Food Chemistry 54: 480–484. DOI: 10.1021/jf0523014.
 
3.
Bayraktar H., Fatma D. 2010. Molecular identification and genetic diversity of Fusarium species associated with onion fields in Turkey 159 (1): 28–34. DOI: 10.1111/j.1439-0434.2010.01715.x.
 
4.
Belkum A., Quint W.V., Pauw B.E., Melchers W.G., Meis J.F. 1993. Typing of Aspergillus species and Aspergillus fumigatus isolates by inter repeat polymerase chain reaction. Journal of Clinical Microbiology 31: 2502–2505.
 
5.
Blandino M., Haidukowski M., Pascale M., Plizzari L., Scudellari D., Reyneri A. 2012. Integrated strategies for the control of Fusarium head blight and deoxynivalenol contamination in winter wheat. Field Crops Research 133: 139–149. DOI: https://doi.org/10.1016/j.fcr.....
 
6.
Booth C. 1971. The Genus Fusarium. The Commonwealth Mycological Institute, Kew, United Kingdom, 237 pp.
 
7.
Burgess W., Backhouse D., Summerell A., Swan J. 2001. Crown rot of wheat. p. 271–294. In: “Fusarium” (Summerell B.A., Leslie J.F., Backhouse D., Bryden W.L., Burgess L.W., eds.). Paul E. Nelson Memorial Symposium. APS Press, St. Paul, Minnesota, USA.
 
8.
Chakraborty S., Liu C.J., Mitter V., Scott B., Akinsanmi O.A., Ali S., Dill-Macky R., Nicol J., Backhouse D., Simpfendorfer S. 2006. Pathogen population structure and epidemiology are keys to wheat crown rot and Fusarium head blight management. Australasian Plant Pathology 35: 643–655. DOI: https://doi.org/10. 1071/ap06068.
 
9.
De Nijs M., Larson S., Gams W., Rombouts M., Wernas K., Thrane U. 1997. Variation in random amplified polymorphic DNA patterns and secondary metabolic profiles within Fusarium species from cereals from various parts of TheNetherlands. Food Microbiology 14 (5): 449–457. DOI: https://doi.org/10.1006/fmic.1....
 
10.
Edel V., Steinberg C., Avelange I., Laguerre G., Alabouvette C. 1995. Comparison of three molecular methods for the characterization of Fusarium oxysporum strains. Phytopathology 85: 579–585.
 
11.
El-Khalifeh M., El-Ahmed A., Al-Saleh A., Nachit M. 2009. Use of AFLPs to differentiate between Fusarium species causing root rot disease on durum wheat (Triticum turgidum L. var. durum). African Journal of Biotechnology 8: 4347–4352.
 
12.
Eslahi M. 2012. Fungi associated with root and crown rot of wheat in Khuzestan province, Iran. Journal of Crop Protection 1 (2): 107–113.
 
13.
Gang G., Miedaner T., Schuhmacher U., Schollenberger M., Geiger H. 1998. Deoxynivalenol and nivalenol production by Fusarium culmorum isolates differing in aggressiveness toward winter rye. Phytopathology 88 (9): 879–884. DOI: https://doi.org/10.1094/phyto.....
 
14.
Gargouri S., Louis B., Mohamed H., Mohamed M. 2003. Genetic variability and population structure of the wheat foot rot fungus, Fusarium culmorum, in Tunisia. European Journal of Plant Pathology 109: 807–815. DOI: https://doi.org/10.1023/A:1026....
 
15.
Gurel F., Albayrak G., Diken O., Cepni E., Tunali B. 2010. Use of REP-PCR for genetic diversity analyses in Fusarium culmorum. Journal of Phytopathology 158 (5): 387–389. DOI: https://doi.org/10.1111/j.1439....
 
16.
Hajieghrar B. 2009. Wheat crown and root rotting fungi in Moghan area, Northwest of Iran. African Journal of Biotechnology 8 (22): 6214–6219. DOI: https://doi.org/ 10.5897/ajb09.1087.
 
17.
Ji F., Wu J., Zhao H., Xu J., Shi J. 2015. Relationship of deoxynivalenol content in grain, chaff, and straw with Fusarium head blight severity in wheat varieties with various levels of resistance. Toxins 7: 728–742. DOI: https://doi.org/10.3390/ toxins7030728.
 
18.
Khalifah M., Oadi N.M. 2014. Pathogenicity evaluation of Fusarium spp. isolates, causal agent of crown rot disease in wheat. The Iraqi Journal of Agricultural Sciences 44 (4): 480–489.
 
19.
Kollers S., Rodemann B., Ling J., Korzun V., Ebmeyer E., Argillier O., Hinze M., Plieske J., Kulosa D., Ganal M.W., Röder M.S. 2013. Whole genome association mapping of Fusarium head blight resistance in european winter wheat (Triticum aestivum L.). PLoS One 8 (2): e57500. DOI: https://doi.
 
20.
Kovach L. 2001. MVSP: Multivariate statistical package v3.12f. Kovach Computing Services, Pentraeth, Anglesey, UK.
 
21.
Leslie J., Summerell B.A. 2006. The Fusarium Laboratory Manual. Blackwell Publisher, London, 388 pp. DOI: 10.1002/9780470278376.
 
22.
Llorens A., Hinojo M., Mateo R., González-Jaén M., Valle-Algarra F., Logrieco A., Jiménez M. 2006. Characterization of Fusarium spp. isolates by PCR-RFLP analysis of the intergenic spacer region of the rRNA gene (rDNA). International Journal of Food Microbiology 106 (3): 297–306. DOI: https://doi.org/10.1016/ j.ijfoodmicro.2005.09.005.
 
23.
Matny O. 2015. Fusarium head blight and crown rot on wheat and barley: losses and health risks. Advances in Plants and Agriculture Research 2 (1): 00039. DOI: https://doi.org/10.15406/apar.....
 
24.
Matny O., Bates S.T., Song Z. 2017. Geographic distribution of Fusarium culmorum chemotypes associated with wheat crown rot in Iraq. Journal of Plant Protection Research 57 (1): 43–49. DOI: https://doi.org/10.1515/jppr-2....
 
25.
Matny O., Sukumar C., Friday O., Rakib A. 2012. Molecular identification of Fusarium spp. causing crown rot and head blight on winter wheat in Iraq. Journal of Agricultural Technology 8 (5): 1677–1690.
 
26.
Matny O., Khalifah M. 2015. Biological control of crown rot disease caused by Fusarium graminearum on wheat in Iraq. The Iraqi Journal of Agricultural Sciences 46 (6): 984–997.
 
27.
McDonald A., Linde C. 2002. The population genetics of plant pathogens and breeding for durable resistance. Euphytica 124: 163–180.
 
28.
Miedaner T., Würschum T., Maurer H., Korzun V., Ebmeyer E., Reif J.C. 2011. Whole genome association mapping for Fusarium head blight resistance in European soft winter wheat. Molecular Breeding 28 (4): 647–655. DOI: https://doi.org/10.1007/s11032....
 
29.
Mishra K., Fox T., Cutham A. 2003. Inter-simple sequence repeat and aggressiveness analyses revealed high genetic diversity, recombination and long range dispersal in Fusarium culmorum. Annals of Applied Biology 143 (3): 291–301. DOI: https://doi.org/10.1111/j.1744....
 
30.
Mitter V., Zhang C., Liu J., Ghosh R., Ghosh M., Chakrabort S. 2006. A high-throughput glasshouse bioassay to detect crown rot resistance in wheat germplasm. Plant Pathology 55: 433–441. DOI: 10.1111/j.1365-3059.2006.01384.x.
 
31.
Nicholson P., Jenkinson P., Rezanoor N., Parry W. 1993. Restriction fragment length analysis of variation in Fusarium species causing ear blight of cereals. Plant Pathology 42 (6): 905–914. DOI: https://doi.org/10.1111/j.1365....
 
32.
O’Donnell K., Kistler C., Tacke K., Casper H. 2000. Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab. Proceedings of the National Academy of Sciences, USA, 97 (14): 7905–7910. DOI: https://doi.org/10.1073/ pnas.130193297.
 
33.
Pestka J., Smolinski T. 2005. Deoxynivalenol: toxicology and potential effects on humans. Journal of Toxicology and Environmental Health, Part B 8 (1): 39–69. DOI: https://doi.org/10.1080/109374....
 
34.
Polley W., Turner A. 1995. Surveys of stem base diseases and Fusarium ear diseases in winter wheat in England, Wales and Scotland 1989–1990. Annals of Applied Biology 126 (1): 49–59. DOI: https://doi.org/10.1111/j.1744... 2.x.
 
35.
Puhalla E. 1981. Genetic considerations of the genus Fusarium. p. 291–305. In: “Fusarium Diseases, Biology, and Taxonomy” (Nelson P.E., Toussoun T.A, Cook R.J., eds.). University Park, Pennsylvania, Pennsylvania State University Press, PA, USA.
 
36.
Saharan M., Naef A. 2008. Detection of genetic variation among Indian wheat head scab pathogens (Fusarium spp. isolates) with microsatellite markers. Crop Protection 27 (7): 1148–1154. DOI: https://doi.org/10.1016/j.crop....
 
37.
Scherm B., Balmas V., Spanu F., Pani G., Delogu G., Pasquali M., Migheli Q. 2013. Fusarium culmorum: Causal agent of foot and root rot and head blight on wheat. Molecular Plant Pathology 14 (4): 323–341. DOI: https://doi.org/10.1111/mpp .12011.
 
38.
Smiley R., Gourlie J., Easley S., Patterson M., Whittaker R. 2005. Crop damage estimates for crown rot of wheat and barley in the Pacific Northwest. Plant Disease 89 (6): 595–604. DOI: https://doi.org/10.1094/pd-89-....
 
39.
Tamura K.., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. DOI: https://doi.org/10.1093/molbev....
 
40.
Tunali B., Nicol J., Erol Y., Altiparmak G. 2006. Pathogenicity of Turkish crown and head scab isolates on stem bases on winter wheat under greenhouse conditions. Plant Pathology Journal 5 (2): 143–149. DOI: https://doi.org/10.3923/ppj.20.... 143.149.
 
41.
Tunali B., Obanor F., Ergibnas G., Westecott R.A., Nicole J., Chakraborty S. 2012. Fitness of three Fusarium pathogens of wheat. FEMS Microbiology Ecology 81 (3): 596–609. DOI: ttps://doi.org/10.1111/j.1574-6941.20....
 
42.
Versalovic J., Koeuth T., Lupski R. 1991. Distribution of repetitive DNA sequences in eubacteria and application tofingerprinting of bacterial genomes. Nucleic Acids Research 19 (24): 6823–6831. DOI: https://doi.org/10.1093/nar/19....
 
43.
Winter M., Koopmann B., Döll K., Karlovsky P., Kropf U., Schlüter K., Tiedemann A. 2013. Mechanisms regulating grain contamination with trichothecenes trans-located from the stem base of wheat (Triticum aestivum) infected with Fusarium culmorum. Phytopathology 103 (7): 682–689. DOI: https://doi.org/10.1094/phyto-....
 
44.
Yörük E., Albayrak G. 2013. Genetic characterization of Fusarium graminearum and F. culmorum isolates from Turkey by using random-amplified polymorphic DNA. Genetics and Molecular Research 12 (2): 1360–1372. DOI: https://doi.org/10. 4238/2013.april.25.7.
 
45.
Yörük E., Tunali B., Kansu B., Ölmez F., Uz G., Zümrüt I.M., Sarıkaya A., Meyva G. 2016. Characterization of high-level deoxynivalenol producer Fusarium graminearum and F. culmorum isolates caused head blight and crown rot diseases in Turkey. Journal of Plant Diseases and Protection 123 (4): 177–186. DOI: https://doi.org/10.1007/s41348....
 
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