ORIGINAL ARTICLE
Genotypic differences between isolates of Phytophthora cinnamomi Rands and P. citricola Sawada obtained from twelve nursery plant species
 
More details
Hide details
1
Research Institute of Pomology and Floriculture Pomologiczna 18, 96-100 Skierniewice, Poland
2
Central Laboratory of State Plant Health and Seed Inspection Service Żwirki i Wigury 73, 87-100 Toruń, Poland
CORRESPONDING AUTHOR
Katarzyna Wiejacha
Research Institute of Pomology and Floriculture Pomologiczna 18, 96-100 Skierniewice, Poland
 
Journal of Plant Protection Research 2004;44(3):189–198
KEYWORDS
TOPICS
ABSTRACT
Genotypic differentiation among 10 isolates of Phytophthora cinnamomi Rands and 24 isolates of Phytophthora citricola Sawada from 12 different plant species grown in Polish ornamental nurseries was determined. DNA was extracted from pure pathogen cultures and amplified by the PCR technique using ISSR and RAPD primers. 9 primers were used to amplify P. cinnamomi and 8 to amplify P. citricola DNA. The analyzed amplification products were between 300 and 2300 bp. The genotypical differentiation was from 17 to 35% in P. cinnamomi and from 10 to 60% in P. citricola. Isolates from host plants of the same family showed, with some exceptions, similar levels of differentiation.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (12)
1.
Aljanabi S.M., Martinez I. 1997. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res., 25: 4692–4693.
 
2.
Brasier C.M. 1992. Evolutionary biology of Phytophthora. Part I: Genetic system, sexuality and the generation of variation. Ann. Rev. Phytopathol., 30: 153–171.
 
3.
Brasier C.M., Hansen E.M. 1992. Evolutionary biology of Phytophthora. Part II: Phylogeny,speciation, and population structure. Ann. Rev. Phytopathol., 30: 173–200.
 
4.
Chang T.T., Yang W.W., Wang W.Y. 1996. Use of random amplified DNA markers for the detection of genetic variation of Phytophthora cinnamomi in Taiwan. Bot. Bull. Acad. Sin., 37: 165–171.
 
5.
Erwin D.C., Ribeiro O.K. 1996. Phytophthora. Diseases worldwide. The American Phytopathological Society, S. Paul, USA, 269 pp.
 
6.
Förster H., Coffey M.D. 1991. Approaches to the taxonomy of Phytophthora using polymorphisms in mitochondrial and nuclear DNA. p. 164–183. In “Phytophthora Symposium of the British Mycological Society, the British Society for Plant Pathology Abd the Society of Irish Plant Pathologists Held”(J.A. Lucas, R.C. Shattock, D.S. Shaw, J.D. Lucas, L.R. Cooke, eds.). Cambridge University Press.
 
7.
Hüberli D., Tommerup I.C., Dobrowolski M.P., Calver M.C., Hardy G.E.St.J. 2001. Phenotypic variation in clonal lineage of two Phytophthora cinnamomi populations from Western Australia. Mycol. Res., 105: 1053–1064.
 
8.
Lee J.S., Lee P.O., Roh M.S. 1996. Classification of lilies using Random Ampliefied Polymorphic DNA (RAPD) analysis. Acta Horticulturae 414: 137–143.
 
9.
Lisek A. 2001. Opracowanie metod przechowywania pędów odmian truskawki i maliny w kulturach in vitro dla celów produkcji elitarnego materiału. Praca doktorska, Instytut Sadownictwa i Kwiaciarstwa, Skierniewice, p 19.
 
10.
Nei M., Li W.H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA, 74: 5267–5273.
 
11.
Orlikowski L.B., Szkuta G. 2002. Fytoftorozy w szkółkach roślin ozdobnych w Polsce. Prace IBL, Ser. A., 2: 134–144.
 
12.
Wiejacha K., Szkuta G., Orlikowska T. 2002. Optimization of DNA isolation procedure as the first step in identification of Phytophthora spp.. Bull. Polish Acad. Sci., 50: 165–171.
 
eISSN:1899-007X
ISSN:1427-4345