ORIGINAL ARTICLE
Multigene phylogeny reveals three new records of Colletotrichum spp. and several new host records for the mycobiota of Iran
 
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Plant Protection Department, Faculty of Agriculture, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran
 
 
Submission date: 2015-01-08
 
 
Acceptance date: 2015-05-08
 
 
Corresponding author
Mahdi Arzanlou
Plant Protection Department, Faculty of Agriculture, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran
 
 
Journal of Plant Protection Research 2015;55(2):198-211
 
KEYWORDS
TOPICS
ABSTRACT
The genus Colletotrichum comprises a number of plant pathogens of major importance which cause anthracnose diseases on a wide range of woody and herbaceous plants worldwide. With the advent of molecular studies, it has been shown that most of the previously known species e.g. C. boninense, C. acutatum, and C. gloeosporioides have been split into several species. In the present study, the identity of Colletotrichum isolates from the northern and northwestern zone of Iran were determined based on multi-gene phylogenetic analyses. Phylogenetic analysis based on a combination of internal transcribed spacer (ITS), beta tubulin (TUB), histone H3 (HIS), calmodulin (CAL), and actin (ACT) loci, clustered our isolates into three clades, including C. salicis on Salix sp., Colletotrichum sp. (C. fuscum sensu lato) within the C. destructivum species complex on Viola sp., and C. fructicola on Citrus sinensis, Malus domestica, Gleditsia caspica, and Sambucus ebulus. These three species are new for mycobiota of Iran. According to these results, Viola sp. from West Azerbaijan (Khoy-Firouragh) is a new host for Colletotrichum sp. in the C. destructivum species complex. Furthermore, C. sinensis from Mazandaran (Behshahr), and G. caspica, and S. ebulus from Guilan (Talesh), are new host records for C. fructicola.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (42)
1.
Alizadeh A., Javan-Nikkhah M., Pordel A. 2013. First report of Colletotrichum caudatum from pampas grass plant in Iran. p. 96. In: Proceedings of the 1st Iranian Mycological Congress, University of Guilan, Rasht, Iran, 3–5 September 2013, 205 pp.
 
2.
Arzanlou M., Khodaei S. 2012a. Phenotypic and molecular characterization of Chaetopyrena Penicillata from Iran with description of a hyphomycete synanomorph. Mycosphere 3 (1): 73–77.
 
3.
Arzanlou M., Khodaei S. 2012b. Aureobasidium iranianum, a new species on bamboo from Iran. Mycosphere 3 (4): 404–408.
 
4.
Arzanlou M., Torbati M. 2013. Phenotypic and molecular characterization of Colletotrichum acutatum, the casual agent of anthracnose disease on Cornus mas in Iran. Archives of Phytopathology and Plant Protection 46 (7): 518–525.
 
5.
Arzanlou M., Narmani A. 2014. Multiplex PCR assay for specific identification and determination of mating type applied to Togninia minima(anamorph known as Phaeoacremonium aleophilum), one of the causal agents of esca disease in grapevine. Phytopathologia Mediterranea53 (2): 240–249.
 
6.
Arzanlou M., Narmani A. 2015. ITS sequence data and morphology differentiate Cytospora chrysosperma associated with trunk disease of grapevine in northern Iran. Journal of Plant Protection Research 55 (2): 117₋125.
 
7.
Arzanlou M., Moshari S., Salari M., Badali H. 2013. Molecular characterization and pathogenicity Phaeoacremonium spp. associated with esca disease of grapevine in northern Iran. Archives of Phytopathology and Plant Protection 46 (4): 375–388.
 
8.
Bakhshi M., Arzanlou M., Babai-ahari A. 2011. Uneven distribution of mating type alleles in Iranian populations of Cercospora beticola, the causal agent of Cercospora leaf spot disease of sugar beet. Phytopatholgia Mediterranea 50 (1): 101–109.
 
9.
Bakhshi M., Arzanlou M., Babai-ahari A., Groenewald J.Z., Braun U., Crous P.W. 2015. Application of the consolidated species concept to Cercospora spp. from Iran. Persoonia 34: 65–86.
 
10.
Bakhshi M., Arzanlou M., Babai-ahari A., Groenewald J.Z., Crous P.W. 2014. Multi-gene analysis of Pseudocercospora spp. from Iran. Phytotaxa 184 (5): 245–264.
 
11.
Cannon P.F., Damm U., Johnston P.R., Weir B.S. 2012. Colletotrichum current status and future directions. Studies in Mycology 73 (1): 181–213.
 
12.
Carbone I., Kohn L.M. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91 (3): 553–556.
 
13.
Crous P.W., Groenewald J.Z., Mansilla J.P., Hunter G.C., Wingfield M.J. 2004. Phylogenetic reassessment of Mycosphaerella spp. and their anamorphs occurring on Eucalyptus. Studies in Mycology 50: 195–214.
 
14.
Crous P.W., Verkley G.J.M., Groenewald J.Z., Samson R.A. 2009. Fungal Biodiversity. CBS Laboratory Manual Series 1,Centraalbureau voor Schimmelcultures, Utrecht, Netherlands, 269 pp.
 
15.
Damm U., Woudenberg J.H.C., Cannon P.F., Crous P.W. 2009. Colletotrichum species with curved conidia from herbaceous hosts. Fungal Diversity 39: 45–87.
 
16.
Damm U., Baroncelli R., Cai L., Kubo Y., O’Connell R., Weir B., Yoshino K., Cannon P.F. 2010. Colletotrichum: species, ecology and interactions. IMA Fungus 1 (2): 161–165.
 
17.
Damm U., Cannon P.F., Woudenberg J.H.C., Crous P.W. 2012. The Colletotrichum acutatum species complex. Studies in Mycology 73 (1): 37–113.
 
18.
Damm U., O’Connell R.J., Groenewald J.Z., Crous P.W. 2014. The Colletotrichum destructivum species complex – hemibiotrophic pathogens of forage and field crops. Studies in Mycology 79: 49–84.
 
19.
Davari M., van Diepeningen A.D., Babai-Ahari A., Arzanlou M., Najafzadeh M.J., van der Lee T.A., de Hoog G.S. 2012. Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA). Journal of Microbiological Methods 89 (1): 63–70.
 
20.
de Hoog G.S., Gerrits van den Ende A.H.G. 1998. Molecular diagnostics of clinical strains of filamentous Basidiomycetes. Mycoses 41 (5–6): 183–189.
 
21.
Drummond A.J., Ashton B., Buxton S., Cheung M., Cooper A., Duran C., Heled J,. Kearse M., Markowitz S., Moir R., Stones-Havas S., Sturrock S., Swidan F., Thierer T., Wilson A. 2012. Geneious v5.6. http://www.geneious.com [Accessed: March 2, 2014].
 
22.
Ershad D. 2009. Fungi of Iran. 3rd ed. Agricultural Research, Education and Extension Organization Publishing, 874 pp.
 
23.
Glass N.L., Donaldson G.C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. Applied and Environmental Microbiology 61 (4): 1323–1330.
 
24.
Hyde K.D., Cai L., Cannon P.F., Crouch J.A., Crous P.W., Damm U., Goodwin P.H., Chen H., Johnston P.R., Jones E.B.G., Liu Z.Y., McKenzie E.H.C., Moriwaki J., Noireung P., Pennycook S.R., Pfenning L.H., Prihastuti H., Sato T., Shivas R.G., Tan Y.P., Taylor P.W.J., Weir B.S., Yang Y.L., Zhang J.Z. 2009. Colletotrichum– names in current use. Fungal Diversity 39: 147–183.
 
25.
Katoh K., Toh H. 2008. Recent developments in the MAFFT multiple sequence alignment program. Brief Bioinformatics 9 (4): 286–298.
 
26.
Li H., Jiang J., Hong N., Wang G., Xu W. 2013. First report of Colletotrichum fructicola causing bitter rot of pear (Pyrus bretschneideri) in China. Plant Disease 97: 1000.
 
27.
Manire C.A., Rhinehart H.L., Sutton D.A., Thompson E.H., Rinaldi M.G., Buck J.D., Jacobson E. 2002. Disseminated mycotic infection caused by Colletotrichum acutatum in Kemp’s Ridley sea turtle (Lepidochelys kempi). Journal of Clinical Microbiology 40 (1): 4273–4280.
 
28.
Moller E.M., Bahnweg G., Geiger H.H. 1992. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Results 20 (22): 6115–6116.
 
29.
Nylander J.A.A. 2004. MrModeltest v2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden.
 
30.
O’Donnell K., Cigelnik E. 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution 7 (1): 103–116.
 
31.
Prihastuti H., Cai L., Chen H., McKenzie E.H.C., Hyde K.D. 2009. Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity 39: 89–109.
 
32.
Quaedvlieg W., Binder M., Groenewald J.Z., Summerell B.A., Carnegie A.J., Burgess T.I., Crous P.W. 2014. Introducing the consolidated species concept to resolve species in the Teratosphaeriaceae. Persoonia 33: 1–40.
 
33.
Ritterband D.C., Shah M., Seedor J.A. 1997. Colletotrichum graminicola: a new corneal pathogen. Cornea 16 (3): 362–364.
 
34.
Ronquist F., Huelsenbeck J.P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19 (12): 1572–1574.
 
35.
Sharma G., Shenoy B.D. 2014. Colletotrichum fructicola and C. siamense are involved in chilli anthracnose in India. Archives of Phytopathology and Plant Protection 47 (10): 1179–1194.
 
36.
Shiraishi A., Araki-Sasaki K., Mitani A., Miyamoto H., Sunada A., Ueda A., Asari S., Zheng X., Yamamoto Y., Hara Y., Ohashi Y. 2011. Clinical characteristics of keratitis due to Colletotrichum gloeosporioides. Journal of Ocular Pharmacology and Therapeutics 27 (5): 487–491.
 
37.
Shivaprakash M.R., Appannavar S.B., Dhaliwal M., Gupta A., Gupta S., Gupta A., Chakrabarti A. 2011. Colletotrichum truncatum: an unusual pathogen causing mycotic keratitis and endophthalmitis. Journal of Clinical Microbiology 49 (8): 2894–2898.
 
38.
Sutton B.C. 1980. The Coelomycetes. Fungi Imperfecti with Pycnidia, Acervuli and Stromata. Commonwealth Mycological Institute, Kew, UK, 696 pp.
 
39.
Sutton B.C. 1992. The genus Glomerella and its anamorph Colletotrichum. p. 1–23. In: “Colletotrichum: Biology, Pathology and Control” (J.A. Bailey, M.J. Jeger, eds.). CAB International, Wallingford, Oxon, UK, 416 pp.
 
40.
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA v5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution 28 (10): 2731–2739.
 
41.
Weir B.S., Johnston P.R., Damm U. 2012. The Colletotrichum gloeosporioides species complex. Studies in Mycology 73 (1): 115–180.
 
42.
White T.J., Bruns T., Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. p. 315–322. In: “A guide to molecular methods and applications” (M.A. Innis, D.H. Gelfand, J.J. Sninsky, J.W. White, eds.). Academic Press, New York, USA, 482 pp.
 
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