ORIGINAL ARTICLE
Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.)
Roksana Skowrońska 1, A-D  
,   Agnieszka Tomkowiak 1, E-F
,   Justyna Szwarc 1, E,   Jerzy Nawracała 1, E-F
,   Michał Kwiatek 1, A,C,E-F
 
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Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Roksana Skowrońska   

Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, Poland
Submission date: 2020-06-30
Acceptance date: 2020-09-03
Online publication date: 2020-11-06
 
Journal of Plant Protection Research 2020;60(4):388–398
 
KEYWORDS
TOPICS
ABSTRACT
Currently, production of wheat cultivars (Triticum aestivum L.) that show durable field resistance against fungal pathogens is a priority of many breeding programs. This type of resistance involves race-nonspecific mechanisms and can be identified at adult-plant stages. Until now, seven genes (Lr34/Yr18, Lr46/Yr29, Lr67/Yr46, Lr68, Lr75, Lr77 and Lr78) conferring durable types of resistance against multiple fungal pathogens have been identified in the wheat gene pool. In this study we showed a multiplex Polymerase Chain Reaction (multiplex PCR) assay, which was developed for detection of slow rusting resistance genes Lr34, Lr46, Lr68, using molecular markers: csLV34, Xwmc44 and csGS, respectively. Identification of molecular markers was performed on 40 selected wheat genotypes which are the sources of slow rusting genes according to literature reports. Multiplex PCR is an important tool to reduce the time and cost of analysis. This multiplex PCR protocol can be applicable for genotyping processes and marker assisted resistance breeding of wheat.
ACKNOWLEDGEMENTS
The authors would like to acknowledge and thank Dr. Harrold Bockelman at the USDA/ARS Small Grains Laboratory, Aberdeen (ID, USA) for providing the seeds of wheat accessions. In addition, we would like to thank all of the reviewers and manuscript editor for their careful review of the manuscript and for their excellent suggestions for improving our initial work.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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