ORIGINAL ARTICLE
Mycotoxins biosynthesis by Fusarium oxysporum and F. proliferatum isolates of asparagus origin
 
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1
Poznań University of Life Sciences Department of Chemistry, Wojska Polskiego 75, 60-625 Poznań, Poland
2
Poznań University of Life Sciences Department of Phytopathology, Dąbrowskiego 159, 60-594 Poznań, Poland
3
Poznań University of Life Sciences Department of Mathematical and Statistical Methods Wojska Polskiego 28, 60-637 Poznań, Poland
CORRESPONDING AUTHOR
Zbigniew Weber
Poznań University of Life Sciences Department of Phytopathology, Dąbrowskiego 159, 60-594 Poznań, Poland
 
Journal of Plant Protection Research 2009;49(4):369–372
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ABSTRACT
The subject of this work was fumonisin B1 (FB1) and moniliformin (MON) biosynthesis by three isolates of Fusarium oxysporum and three isolates of F. proliferatum of asparagus spears origin. The cultures of fungi were grown on rice and asparagus media for 3 weeks at 20oC. Experiment was carried out in 3 replicates. FB 1 and MON occurrence was evaluated with high-performance liquid chromatography (HPLC) analyses. Analysis of variance was carried out to determine biosynthesis of FB 1 and MON by F. oxysporumand F. proliferatum. FB 1 was found in the amount up to 2012.8 μg/g in cultures of F. proliferatum on rice and in a very small amount in two cultures on asparagus medium. F. oxysporum did not produce FB 1 on any of the media. MON was biosynthesized by two the same isolates of F. oxysporum in the amount up to 182.8 μg/g on rice and up to 743.3 μg/g on asparagus medium and one isolate (different on each medium) of F. proliferatum.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (23)
1.
Baayen R.P., Boogert van den P.H.J.F., Bonants P.J.M., Poll J.T.K., Blok W.J., Waalwijk C. 2000. Fusarium redolens f. sp. asparagi, causal agent of asparagus root rot, crown rot and spear rot. Eur. J. Plant Pathol. 106: 907–912.
 
2.
Blok W.J., Bollen G.J. 1995. Fungi on roots and stem bases of asparagus in the Netherlands: species and pathogenicity. Eur. J. Plant Pathol. 101: 15–24.
 
3.
Bogartz R.S. 1994. An Introduction to the Analysis of Variance. Westport, CT, Praeger, 565 pp.
 
4.
Chełkowski J., Zawadzki M., Zajkowski P., Logrieco A., Bottalico A. 1990. Moniliformin production by Fusarium species. Mycotoxin Res. 6: 41–44.
 
5.
Chulze S.N., Ramirez M.L., Farnochi M.C., Pascale M., Visconti A., March G. 1996. Fusarium and fumonisin occurrence in Argentinian corn at different ear maturity stages. J. Agr. Food Chem. 44: 2797–2801.
 
6.
Doko M.B., Visconti A. 1994. Occurrence of fumonisins B1 and B2 in corn and corn-based human foodstuffs in Italy. Food Addit. Contam. 11: 433–439.
 
7.
Elmer W.H. 2000. Incidence of infection of asparagus spears marketed in Connecticut by Fusarium spp. Plant Dis. 84: 831–834.
 
8.
Elmer W.H., Johnson D.A., Mink G.I. 1996. Epidemiology and management of the diseases causal to asparagus decline. Plant Dis. 80: 117–125.
 
9.
Goliński P., Kiecana I., Kaczmarek Z., Kostecki M., Kaptur P., Wiśniewska H., Chełkowski J. 1999. Scab response of selected winter wheat cultivars after inoculation with Fusarium avenaceum (Fr.) Sacc. J. Phytopathol. 147: 717–723.
 
10.
Gossmann M., Büttner C., Bedlan G. 2001. Untersuchungen zum Spargel (Asparagus officinalis L.) aus Jung- und Ertragsanlagen in Deutschland und Österreich auf Infektionen mit FusariumArten. Pflanzenschutzberichte 59: 45–54.
 
11.
International Agency for Research on Cancer (IARC). 2002. Fumonisin B1. Monographs on the evaluation of the carcinogenic risks to humans: Some traditional herbal medicines, some mycotoxins, naphtalene and styrene. Lyon, France 82: 301–333.
 
12.
Kostecki M., Grabarkiewicz-Szczęsna J., Chełkowski J., Wiśniewska H. 1995. Beauvericin and moniliformin production by Polish isolates of Fusarium subglutinans and natural co-occurrence of both mycotoxins in maize samples. Microbiol. Alim. Nutrit. 13: 67–70.
 
13.
Liu C., Xu W., Liu F., Jiang S. 2007. Fumonisins production by Fusarium proliferatum strains isolated from asparagus crown. Mycopathology 164: 127–134.
 
14.
Logrieco A., Doko B., Moretti A., Frisullo S., Visconti A. 1998. Occurrence of fuminisins B1 and B2 in Fusarium proliferatum infected asparagus plants. J. Agr. Food Chem. 46: 5201–5204.
 
15.
Marin S., Magan N., Serra J., Ramos A.J., Canela R., Sanchis V. 1999a. Fumonisin B1 production and growth of Fusarium moniliforme and Fusarium proliferatum on maize, wheat, and barley grain. J. Food Protect. 64: 921–924.
 
16.
Marin S., Magan N., Belli N., Ramos A.J. Canela R., Sanchis V. 1999b. Two-dimensional profiles of fumonisin B1 production by Fusarium moniliforme and Fusarium proliferatum in relation to environmental factors and potential for modelling toxin formation in maize grain. Int. J. Food Microbiol. 51: 159–167.
 
17.
Sanchis V., Abadias M., Oncins L., Sala N., Canela R., Vinas I. 1995. Fumonisin B1 and B2 and toxigenic Fusarium strains in feeds from the Spanish market. Int. J. Food Microbiol. 27: 37–44.
 
18.
Sharman M., Gilbert J., Chełkowski J. 1991. A survey of the occurrence of the mycotoxin moniliformin in cereal samples from sources worldwide. Food Addit. Contam. 4: 459–466.
 
19.
Shephard G.S., Sydenham E.W., Thiel P.G., Gelderblom W.C.A. 1990. Quantitative determination of fumonsin B1 and B2 by highperformance liquid chromatography with fluorescence detection. J. Liq. Chromatogr. 13: 2077–2087.
 
20.
Sydenham E.W., Gelderblom W.C.A., Thiel P.G., Marasas W.F.O. 1990. Evidence for natural occurrence of fuminisin B1, a mycotoxin produced by Fusarium moniliforme, in corn. J. Agric. Food. Chem. 38: 285–290.
 
21.
Sydenham E.W., Shephard G.S., Thiel P.G., Marasas W.F.O., Rheeder J.P., Perlta Sanhueza C.E., Gonzales H.H.L., Resnik S.L. 1993. Fumonisins in Argentinian field-trial corn. J. Agric. Food Chem. 41: 891–895.
 
22.
Weber Z., Kostecki M., Bargen von S., Gossmann M., Waśkiewicz A., Bocianowski J., Knaflewski M., Büttner C., Goliński P. 2006. Fusarium species colonizing spears and forming mycotoxins in field samples of asparagus from Germany and Poland. J. Phytopathol. 154: 209–216.
 
23.
Weber Z., Karolewski Z., Irzykowska L., Knaflewski M., Kosiada T. 2007. Occurrence of Fusarium species in spears of asparagus (Asparagus officinalis). Phytopathol. Pol. 45: 9–15.
 
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