ORIGINAL ARTICLE
Pepino mosaic virus - a pathogen of tomato crops in Poland: biology, evolution and diagnostics
 
More details
Hide details
1
Institute of Plant Protection – National Research Institute, Department of Virology and Bacteriology Węgorka 20, 60-318 Poznań, Poland
2
Institute of Bioorganic Chemistry, Polish Academy of Sciences Noskowskiego 12/14, 61-704 Poznań, Poland
CORRESPONDING AUTHOR
Beata Hasiów-Jaroszewska
Institute of Plant Protection – National Research Institute, Department of Virology and Bacteriology Węgorka 20, 60-318 Poznań, Poland
 
Journal of Plant Protection Research 2010;50(4):470–476
KEYWORDS
TOPICS
ABSTRACT
Pepino mosaic virus (PepMV) has the potential to cause serious tomato disease. Several isolates of PepMV were collected between 2003–2010 in the Department of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznań, Poland. The isolates were obtained from greenhouse tomato plants (Solanum lycopersicum) collected from different regions of Poland. Clear biological and genetic differences were detected among the Polish PepMV isolates. Since the latter induced a wide range of symptoms on tested plant species, five different pathotypes were distinguished. Sequence analysis showed that the Polish PepMV isolates share a very high sequence identity with those representing European and Chilean 2 genotype. The analysis of the genetic diversity of the Polish PepMV CH2 isolates suggested that each of them exists as a genetically diverse collection of variants called a quasispecies.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (50)
1.
Aguilar J.M., Hernández-Gallardo M.D., Cenis J.L., Lacasa A., Aranda M.A. 2002. Complete sequence of the Pepino mosaic virus RNA genome. Arch. Virol. 147 (10): 2009–2015.
 
2.
Alfaro-Fernández A., Medina V., Córdoba-Selles C., Font M.I., Jornet J., Cebrián M.C., Jórda C. 2010. Ultrastructural aspects of tomato leaves infected by Tomato torrado virus (ToTV) and co-infected by other viruses. Plant Pathol. 59 (2): 231–239.
 
3.
Awadalla P. 2003. The evolutionary genomics of pathogen recombination. Nat. Rev. Genet. 4 (50–60): 50–60.
 
4.
Córdoba-Sellés M.C., García-Rández A., Alfaro-Fernández A., Jordá-Gutiérrez C. 2007. Seed transmission of Pepino mosaic virus and efficacy of tomato seed disinfection treatments. Plant Dis. 91 (10): 1250–1254.
 
5.
Cotillon A.C., Girard M., Ducouret S. 2002. Complete nucleotide sequence of the Genomic RNA of a French isolate of Pepino mosaic virus. Arch. Virol. 147 (11): 2231–2238.
 
6.
Davino S., Davino M., Bellardi M.G., Agosteo G.E. 2008. Pepino mosaic virus and Tomato chlorosis virus causing mixed infection in protected tomato crops in Sicily. Phytopathologia Mediterranea 47 (1): 35–41.
 
7.
Desbiez C., Gal-On A., Girard M., Wipf-Scheibel C., Lecoq H. 2003. Increase in Zucchini yellow mosaic virus symptom severity in tolerant zucchini cultivars is related to a point mutation in P3 protein and is associated with a loss of relative fitness on susceptible plants. Phytopathology 93 (12): 1478–484.
 
8.
Divéki Z., Salánki K., Balázs E. 2004. The necrotic pathotype of the Cucumber mosaic virus (CMV) Ns strain is solely determined by amino acid 461 of the 1a protein. Mol. Plant-Microbe Interact. 17 (8): 837–845.
 
9.
French C.J., Bouthillier M., Bernardy M., Ferguson G., Sabourin M., Johnson R.C., Masters C., Godkin S., Mumford R. 2001. First Report of Pepino mosaic virus in Canada and the United States. Plant Dis. 85 (10), p. 1121.
 
10.
Fletcher J.T. 1978. The use of avirulent strains to protect plants against the effects of virulent strains. Ann. Appl. Biol. 89 (1): 110–114.
 
11.
Gómez P., Sempere R.N., Elena S.F., Aranda M.A. 2009. Mixed infections of Pepino mosaic virus strains modulate the evolutionary dynamics of this emergent virus. J. Virol. 23 (83): 12378–12387.
 
12.
Hanssen I.M, Mumford R., Blystad D.G., Cortez I., Hasiów-Jaroszewska B., Dimitrinka H., Pagán I, Pereira A.M, Peters J., Pospieszny H., Ravnikar M., Stijger I., Tomassoli L. , Varveri C., van der Vlugt R., Nielsen S.L. 2010. Seed transmission of Pepino mosaic virus in tomato. Eur. J. Plant Pathol. 126 (2): 145–152.
 
13.
Hanssen I.M., Paeleman A., Wittemans L., Goen K., Lievens B., Bragard C., Vanachter A.C.R.C., Thomma B.P.H.J. 2008. Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination. Eur. J. Plant Pathol. 121 (2): 131–146.
 
14.
Hanssen I, Thomma B. 2010. Pepino mosaic virus: a successful pathogen that rapidly evolved from emerging to endemic in tomato crops. Mol. Plant Pathol. 11 (2): 179–189.
 
15.
Hasiów B., Borodynko N., Pospieszny H. 2008a. Complete genomic RNA sequence of the Polish Pepino mosaic virus isolate belonging to the US2 strain. Virus Genes 36 (1): 209–214.
 
16.
Hasiów B., Borodynko N., Pospieszny H. 2008b. Development of a real time RT-PCR assay for detecting genetically different Pepino mosaic virus isolates. J. Plant Protection Res. 48 (3): 295–301.
 
17.
Hasiów-Jaroszewska B., Borodynko N., Pospieszny H. 2009a. Infectious RNA transcripts derived from cloned cDNA of a Pepino mosaic virus isolate. Arch. Virol. 154 (5): 853–856.
 
18.
Hasiów-Jaroszewska B., Jackowiak P., Borodynko N., Figlerowicz M., Pospieszny H. 2010. Quasispecies nature of Pepino mosaic virus and its evolutionary dynamics. Virus Genes 41 (2): 260–267.
 
19.
Hasiów-Jaroszewska B., Pospieszny H., Borodynko N. 2009b. New necrotic isolates of Pepino mosaic virus representing Ch2 genotype. J Phytopathol. 157 (7–8): 494–496.
 
20.
Jones R.A.C., Koenig R., Lesemann D.E. 1980. Pepino Mosaic Virus a new potexvirus from pepino (Solanum muricatum). Ann. Appl. Biol. 94 (1): 61–68.
 
21.
Jordá C., Lázaro-Pérez A., Martínez-Culebras P.V., Abad-Campos P. 2001. First report of Pepino mosaic virus on tomato in Spain. Plant Dis. 85 (12), p. 1292.
 
22.
Kadare G., Haenni A.L. 1997. Virus-encoded RNA helicases. J. Virol. 71 (4): 2583–2590.
 
23.
Kędziora P., Figlerowicz M., Formanowicz P., Alejska M., Jackowiak P., Malinowska N., Frątczak A., Błażewicz J., Figlerowicz M. 2005. Computational methods in diagnostics of chronic hepatitis C. Bull. Pol. Academy Sci. 53 (3): 273–281.
 
24.
Krinkels M. 2001. PepMV causes sticky problem. Prophyta: 30–33.
 
25.
Ling K.S. 2007. Molecular characterization of two Pepino mosaic virus variants from imported tomato seed reveals high levels of sequence identity between Chilean and US isolates. Virus Genes 34 (1): 1–8.
 
26.
Ling K.S. 2008. Genetic composition of Pepino mosaic virus population in North American greenhouse tomatoes. Plant Dis. 92 (12): 1683–1688.
 
27.
López C., Soler S., Nuez F. 2005. Comparison of the complete sequences of three different isolates of Pepino mosaic virus: size variability of the TGBp3 protein between tomato and L. peruvianum isolates. Arch. Virol. 150 (3): 619–627.
 
28.
Maroon-Lango C.J., Guaragna M.A., Jordan R.L., Hammond J., Bandla M., Marquardt S.K. 2005. Two unique US isolates of Pepino mosaic virus from a limited source of pooled tomato tissue are distinct from a third (European - like) US isolate. Arch. Virol. 150 (6): 1187–1201.
 
29.
Morozov S.Y., Solovyev A.G. 2003. Triple gene block: modular design of a multifunctional machine for plant virus movement. J. Gen. Virol. 84: 1351–1366.
 
30.
Mumford R.A., Metcalfe E.J. 2001. The partial sequencing of genomic RNA of a UK isolate of Pepino mosaic virus and the comparison of the coat protein sequence with other isolates from Europe and Peru. Arch. Virol. 146 (12): 2455–2460.
 
31.
Oshima N. 1975. The control of tomato mosaic disease with attenuated virus of a tomato strain of TMV. Rev. Plant. Prot. Res. 8: 126–135.
 
32.
Owor B., Legg J.P., Okao-Okuja G., Obonyo R., Kyamanywa S., Ogenga-Latigo M.W. 2004. Field studies of cross protection with cassava mosaic geminiviruses in Uganda. J. Phytopathol. 152 (4): 243–249.
 
33.
Pagan I., Córdoba-Sellés M.C., Martínez-Priego L., Fraile A., Malpica J.M., Jordá C., García-Arenal F. 2006. Genetic Structure of the population of Pepino mosaic virus infecting tomato crops in Spain. Phytopathology 96 (3): 274–279.
 
34.
Pospieszny H. 2007. Ochrona krzyżowa w ograniczaniu występowaniu chorób wirusowych. Hasło Ogrodn. 9: 114–116.
 
35.
Pospieszny H., Borodynko N. 2006. New Polish isolate of Pepino mosaic virus highly distinct from European Tomato, Peruvian, and US2 strains. Plant Dis. 90 (8), p. 1106.
 
36.
Pospieszny H., Borodynko N., Palczewska M. 2003. First record of Pepino mosaic virus in Poland. J. Plant Dis. Protect. 100, p. 97.
 
37.
Pospieszny H., Hasiów B., Borodynko N. 2007a. Diagnostyka polskich szczepów wirusa mozaiki pepino. Prog. Plant Protection/Post. Ochr. Roślin 47 (2): 267–270.
 
38.
Pospieszny H., Hasiów B., Borodynko N. 2007b. Nowy szczep wirusa mozaiki pepino (Pepino mosaic virus) na pomidorze szklarniowym. Prog. Plant Protection/Post. Ochr. Roślin 47 (2): 271–279.
 
39.
Pospieszny H., Hasiów B., Borodynko N. 2008. Characterization of two distinct Polish isolates of Pepino mosaic virus. Eur. J. Plant Pathol. 122 (3): 443–445.
 
40.
Rast A.T.B. 1972. MII-16, an artificial symptomless mutant of tobacco mosaic virus for seedling inoculation on tomato crops. Eur. J. Plant Pathol. 78 (3): 110–112.
 
41.
Reed G.H., Kent J.O., Wittwer C.T. 2007. High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics 8 (6): 597–608.
 
42.
Roossinck M.J. 1997. Mechanism of plant virus evolution. Ann. Rev. Phytopathol. 35: 191–209.
 
43.
Salomone A., Roggero P. 2002. Host range, seed transmission and detection by ELISA and lateral flow of an Italian isolate of Pepino mosaic virus. J. Plant Pathol. 84 (1): 65–68.
 
44.
Schneider W.L., Roossinck M.J. 2001. Genetic diversity in RNA virus quasispecies is controlled by host-virus interactions. J. Virol. 75 (14): 6566–6571.
 
45.
Spence N.J., Basham J., Mumford R.A., Hayman G., Edmondson R., Jones D.R. 2006. Effect on Pepino mosaic virus on the yield and quality of glasshouse-grown tomatoes in the UK. Plant Pathol. 55 (5): 595–606.
 
46.
Soler-Aleixandre S., López C., Díez M J., Pérez Castro A., Nuez F. 2005. Association of Pepino mosaic virus with Tomato Collapse. J. Phytopathol. 153: 464–469.
 
47.
Verhoeven J.Th.J, van der Vlugt R.A.A., Roenhorst J.W. 2003. High similarity between tomato isolates of Pepino mosaic virus suggests a common origin. Eur. J. Plant Pathol. 109 (5): 419–425.
 
48.
van der Vlugt R.A.A., Stijger C.C.M., Verhoeven J.T.J., Lesemann D.E. 2000. First report of Pepino mosaic virus on tomato. Plant Dis. 84 (1), p. 103.
 
49.
Wang H.L., Yeh S.D, Chiu R.J., Gonsalves D. 1987. Effectiveness of cross-protection by mild mutants of papaya ringspot virus for control of ringspot disease of papaya in Taiwan. Plant Dis. 71: 491–497.
 
50.
Wright D., Mumford R. 1999. Pepino mosaic Potexvirus (PepMV): first records in tomato in the United Kingdom. Plant Dis. Notice, No. 89, York: Central Science Laboratory.
 
eISSN:1899-007X
ISSN:1427-4345