Postulation of leaf rust resistance genes of 20 wheat cultivars in southern Russia
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Ministry of Science and Higher Education of the Russian Federation, All-Russian Research Institute of Biological Plant Protection, Krasnodar, Russia
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Olga Alexandrovna Kudinova   

Ministry of Education and Science of the Russian Federation, All-Russian Research Institute of Biological Plant Protection, 350039 Krasnodar-39, 350039, Krasnodar, Russia
Submission date: 2020-02-22
Acceptance date: 2020-04-16
Online publication date: 2020-07-28
Journal of Plant Protection Research 2020;60(3):225–232
Gene postulation is one of the fastest and most cost-effective methods for identifying seedling leaf rust resistance genes in wheat cultivars. Many researchers use this approach to identify Lr genes in wheat cultivars. The purpose of our research was to identify seedling leaf rust resistance genes in 20 wheat cultivars from different breeding centers of Russia, Ukraine and Germany. Forty-two near isogenic Thatcher lines and 10 Puccinia triticina isolates were used for gene postulation. When assessing the infection types to cultivars and lines, a scale was used, according to Oelke and Kolmer. In 20 wheat cultivars 19 Lr genes were postulated: 2c, 3, 10, 3bg, 3ka, 14a, 17, 18, 23, 25, 26, 30, 33, 40, 44, 50, B, Exch, Kanred. The most common for cultivars was the Lr10 gene. In five cultivars, showing high field resistance, most postulated seedling genes (Lr2c, Lr3, Lr10, Lr14а, Lr26, Lr33) were not effective in the adult stage. It is possible that resistance of such cultivars is associated with APR genes, the postulation of which requires an expansion in the number and spectrum of P. triticina isolate virulence. Most of the studied cultivars (60%) have recently been entered into the register (2015–2019) and in the field show a stable or moderately susceptible response to P. triticina infection, despite the fact that the Lr genes postulated in them were not effective in the adult stage. The data obtained indicated a variety of genotypes of the studied cultivars, as well as the tendency of breeders to use the effect of pyramiding ineffective genes, which can prolong the resistance of the cultivar. Annual monitoring of varieties is necessary in each region, especially when reacting with a medium susceptible type (MS), which may indicate the initial stage of resistance loss.
The authors are very grateful to Dr. J.A. Kolmer, who first recommended that we postulate genes for Russian varieties. The authors thank the staff of the laboratory for immunity of grain crops to fungal diseases of the All-Russian Scientific Research Institute of Biological Plant Protection for their assistance in conducting research.
The authors have declared that no conflict of interests exist.
The studies were carried out in accordance with State Assignment No. 075-00376-19-00 of the Ministry of Science and Higher Education of the Russian Federation as part of research on the topic No. 0686-2019-0008. The research used the material and technical base and objects of the Unique Scientific Installation “State Collection of Entomophages and Microorganisms” (http://ckp-rf.ru/ registry number 58 58 58).
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