ORIGINAL ARTICLE
Potential of endochitinase gene to control Fusarium wilt and early blight disease in transgenic potato lines
Neelam Fatima 1, B
,  
Bushra Tabassum 1, A,E  
,  
Iqra Yousaf 1, C
,  
Momna Malik 1, B
,  
Nida Toufiq 1, C
,  
Imtiaz Ahmad Sajid 1, B
,  
Saman Riaz 1, D
,  
 
 
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Centre of Excellence in Molecular Biology, University of the Punjab, Lahore-Pakistan, Pakistan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Bushra Tabassum   

Centre of Excellence in Molecular Biology, University of the Punjab, Lahore-Pakistan, Pakistan
Online publish date: 2019-10-09
Submission date: 2019-01-31
Acceptance date: 2019-08-21
 
Journal of Plant Protection Research 2019;59(3):376–382
KEYWORDS
TOPICS
ABSTRACT
Potato (Solanum tuberosum L.), an important food crop in the world, is susceptible to many fungal pathogens including Alternaria solani and Fusarium oxysporum causing Fusarium wilt and early blight diseases. Mycoparasitic fungi like Trichoderma encode chitinases, cell wall degrading enzymes, with high antifungal activity against a wide range of phytopathogenic fungi. In this study, a binary vector harboring endochitinase gene of ~1,000 bp was constructed and used to transform potato nodes through Agrobacterium-mediated transformation. Out of several primary transformants, two transgenic potato lines were verified for transgene insertion and integration by Southern blot. In a pot experiment for Fusarium resistance, the transgenic potato lines didn’t show any symptoms of disease, instead they remained healthy post infection. The transgenic potato lines exhibited 1.5 fold higher mRNA expression of endochitinase at 7 days as compared to 0 day post fungus inoculation. It was evident that the mRNA expression decreased over days of inoculation but was still higher than at 0 day and remained stable upto 30 days post inoculation. Similarly, for A. solani infection assay, the mRNA expression of the endochitinase gene was 3 fold higher 7 days post inoculation compared to expression at 0 day. Although the expression decreased by1.2 fold during subsequent days post infection, it remained stable for 30 days, suggesting that protection in transgenic potato plants against fungal pathogens was achieved through an increase in endochitinase transcript.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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