Protective treatments against soilborne pathogens in citrus orchards
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Department of Plant Pathology, National Research Center, El-Behoos St., 12622, Giza, Egypt
Abdel-Kader Mokhtar M.
Department of Plant Pathology, National Research Center, El-Behoos St., 12622, Giza, Egypt
Journal of Plant Protection Research 2010;50(4):477–484
The efficacy of applying biocontrol agents, chemical fungicide and nematicide as protective treatments against the soilborne parasites, Fusarium spp. (Fusaria) and citrus nematode Tylenchulus semipenetrans Cobb was evaluated. The experiment took place under field conditions in a citrus orchard cultivated with 16-year-old sweet orange (Citrus sinensis L.) osbech cv. Valencia trees grafted on sour orange (C. aurantium L.) rootstock during the growing season November 2006/ October 2007. This orchard is located at Bader district, Behera governorate, Egypt. The populations of soil fauna and flora under trees canopy were examined just before treatment, and 1, 3, 6, 9 and 12 months after the treatment application. A visual inspection for the appearance of symptoms related to Fusarium or nematode infection on treated and untreated citrus trees was carried out periodically every two weeks throughout the experimental period. The populations of Fusarium spp. were gradually decreased throughout the experimental period. However, the antagonistic bacterial isolates showed drastic effect for reducing the Fusaria population from 38.5% before treatments to a range of 1.2–4.0% after one month of such a bacterial application followed by the Kocide (fungicide) treatment which recorded 6.6%. Meanwhile, Fusaria populations of 18.4 and 16.3% were recorded as Fusaria population in treatments of the nematicide Carbofuran and entompathogenic nematode, respectively. Also, the population density of T. semipenetrans juveniles drastically decreased soon after all the treatment applications. Then after the nematode population build up during the growing season followed a natural distribution decline shape starting from the third month of application up to the ninth month, then it decreased. The citrus nematode increased steadily in the untreated check till September 2007 then its population level decreased. Treatments of Bacillus subtilis – B (20 ml) and Pseudomonas fluorescens (20 ml) gave the highest citrus yield followed by B. subtilis A (10 ml); B. subtilis B (10 ml) and Kocide (fungicide). Moreover, citrus trees treated with B. subtilis A (10 ml); P. fluorescens (10 ml) and Carbofuran (nematicide) had a higher yield production than trees treated with entomopathogenic nematodes Heterorhabditis egyptii (Abd-Elgawad and Ameen 2005). Yet, visual monitoring for disease incidence throughout the citrus orchard during the whole period of the study revealed no disease symptoms of any fusaria or nematode infection in treated trees. Untreated trees had a 1.9 and 3.1% fusaria and nematode infection, respectively. The importance of the present work, therefore, is based on the proposed bioagents as protective applications that are able to inhibit the citrus pathogens and prevent them from causing citrus damage.
The authors have declared that no conflict of interests exist.
Abd-Elgawad M.M.M., Ameen H.H. 2005. Heterorhabditis egyptii N.Sp. (Rhabditida: Heterorhabditidae) from Egypt. Egypt. J. Agric. Res. 2 (2): 855–870.
Abd-Elgawad M.M.M., Al-Yahya F.A., Stephan Z.A. 2010. Nematodes of citrus p. 553–602. In: “Plant Nematodes in Arab Countries” (W.A. Abu-Gharbieh, A.S. Al-Hazmi, Z.A. Stephan, A.A. Dawabah, eds.). Darwael for Publishing, Arab Society of Plant Protection, Amman, Jordan.
Agrios G.N. 1988. Plant Pathology. 3rd ed. Academic Press, New York, London, 803 pp.
Al-Rehiayani S. 2006. Suppression of Meloidogyne incognito by soil application of Pasteuria penetrans in Al-Qssim area, Saudi Arabia. 9th Arab. Congress Plant Protect. Damascus, Syria, 19–23 November 2006, 219 pp.
Allen O.N. 1961. Experiments on Soil Bacteriology. Burgess Publishing Co., Minnesota, USA, 214 pp.
Andrew J.H. 1981. Effect of pesticides on non-target microorganisms on leaves. p. 284–304. In: “Microbial Ecology of the Phylloplane” (J.P. Blakmem, ed.). Academic Press, New York, 418 pp.
Anonymous 2008. Yearbook of Statistics of Ministry of Agriculture. Agricultural Economical and Statistical Department, Arab Republic of Egypt, Cairo, 68 pp.
Armstrong G.M., Armstrong J.K. 1975. Reflections on the wilt Fusaria. Ann. Rev. Phytopathol. 13: 95–103.
Barnett H.L., Hunter B.B. 1972. Illustrated Genera of Imperfect Fungi. Burgess Publ. Co., Minnesota, USA, 241 pp.
Becerra L.N. 1989. Efectos del hongo Paecilomyces lilacinus Thom. Samson sobre el nemátodo de la raíz del limón sutil Tylenchulus semi penetraos Cobb en plantaciones comerciales de Olmos. Tesis Ingeniero Agrónomo, Universidad Nacional Pedro Ruiz Gallo, Lambayeque Perú, 84 pp.
Donegan K., Fieland V., Fowles N., Ganio L., Seidler R. 1992. Efficacy of burning, tillage, and biocides in controlling bacteria released at field sites and effects on indigenous bacteria and fungi. Appl. Environ. Microbiol. 58 (4): 1207–1214.
Duncan L.W., Cohn E. 1990. Nematode parasites of citrus. p. 321–346. In: “Plant Parasitic Nematodes in Subtropical and Tropical Agriculture” (M. Luc, R.A. Sikora, J. Bridge, eds.). 1st ed. CABI Publishing, Wallingford, UK.
Duncan L.W. 2005. Nematode parasites of citrus. p. 593–607. In: “Plant Parasitic Nematodes in Subtropical and Tropical Agriculture” (M. Luc, R.A. Sikora, J. Bridge, eds.). 2nd ed. CABI Publishing, Wallingford, UK.
Duncan L.W., Mashela P., Ferguson J., Graham J., Abou-Setta M.M., Morshedy M.M. 1995. Estimation of crop loss in orchards with pathches of mature citrus trees infected by Tylenchulus semipenetrans. Nematropica 25 (1): 43–51.
El-Nagdi W.A., Youssef M.M.A. 2006. Field application of a commercial formulation containing an isolate of Bacillus Thurigiensis for managing Tylenchulus semipenetrans (citris nematode) on Navel orange trees. 9th Arab. Congress Plant Protect. Damascus, Syria, 19–23 November 2006, 211 pp.
Gillespie D.R., Menzies J.G. 1993. Fungus gnat vector Fusarium oxysporum f. sp. radicislycopersici. Ann. Appl. Biol. 123 (3): 539–544.
Grewal P.S., Lewis E.E., Venkatachari S. 1999. Allelopathy: a possible mechanism of supression of plant-parasitic nematodes by entomopathogenic nematodes. Nematology 1 (7–8): 735–743.
Hawes M.C. 1991. Living plant cells released from the root cap: a regulator of microbial populations in the rhizosphere? p. 51–59. In: “The Rhizosphere and Plant Growth” (D.L. Keister, P.B. Cregan, eds.). Boston, MA: Kluwer Academic Publishers.
Kazmar E.R., Goodman R.M., Grau C.R., Johnson D.W., Nordheim E.V., Undersander D.J., Handelsman J.O. 2000. Regression analyses for evaluating the influence of Bacillus cereus on alfalfa yield under variable disease intensity. Phytopathology 90 (6): 657–665.
Kim D.S., Cook R.J., Weller D.M. 1997. Bacillus sp. L324-92 for biological control of three root diseases of wheat grown with reduced tillage. Phytopathology 87 (5): 551–558.
King E.B., Parke J.L. 1993. Biocontrol of Aphanomyces root rot and Pythium damping-off by Pseudomonas cepacia AMMD on four pea cultivars. Plant Dis. 77: 1185–1188.
Labuschange N., Koteze J.M. 1988. Factors affecting feeder root rot of citrus caused by Fusarium solani. Proc. 6th Int. Citrus Congress. Tel Aviv Israel, March 6–11, 1988.
Labuschange N., van der Vegte F.A., Koteze J.M. 1989. Interaction between Fusarium solani and Tylenchulus semipenetrans on citrus roots. Phytophylactica 21 (1): 29–33.
Li T.S.C., Utkhede R.S., Wardle D.A. 1997. Chemical and biological control of leaf blight and root rot caused by Phytophthora cactorum in American ginseng. Can. J. Plant Pathol. 19 (3): 297–300.
Llontop J.A., Carreño C.R. 1999. Comparison of Nematicides for the Control of Tylenchulus semipenetrans on Lemon (Citrus aurantifolia). National University Pedro Ruiz Gallo, Chiclayo, Peru.
Louw H.A., Webely D.W. 1959. The bacteriology of root region of cat plant grown under controlled pot culture conditions. J. Appl. Bacteriol. 22 (2): 216–226.
Manners J.G. 1993. Principles of Plant Pathology. Cambridge Univ. Press, 343 pp.
Mathre D.E., Cook R.J., Callan N.W. 1999. From discovery to use: traversing the world of commercializing biocontrol agents for plant disease control. Plant Dis. 83 (11): 972–983.
Nash S.M., Snyder W.C. 1962. Quantitative estimation by plate counts of propagules of bean root rot Fusarium in field soils. Phytopathology 52 (5): 567–572.
Nemec S. 1975. Microorganisms associated with healthy and sand hill decline roots. Plant Dis. Repter 59 (4): 210–213.
Nemec S., Zablotowice R.M., Chandler J.L. 1989. Distribution of Fusarium spp. and selected microflora in citrus soil and rhizospheres associated with healthy and blight diseased citrus in Florida. Phytophylactica 21 (2): 141–146.
Noling J.W., Ferris H. 1987. Nematode-degree days, a densitytime model for relating epidemiology and crop losses in perennials. J. Nematology 19 (1): 108–118.
Nordmeyer D., Dickson D.W. 1989. Effect of Carbamate, organophosphate, and avermectin nematicides on oxygen consumption by three Meloidogyne spp. J. Nematol. 21: 472–476.
O’Bannon J.H., Radewald J.D., Tomerlin A.T. 1972. Population fluctuation of three parasitic nematodes in Florida. J. Nematol. 4: 194–199.
Papavizas G.C., Lumsden R.D. 1980. Biological control of soilborne fungal propagules. Ann. Rev. Phytopathol. 18: 389–417.
Parke J.L., Rand R.E., Joy A.E., King E.B. 1991. Biological control of Pythium damping-off and Aphanomyces root rot of peas by application of Pseudomonas cepacia or P. fluorescens to seed. Plant Dis. 75 (3): 987–992.
Sikora R.A. 1988. Interrelationships between plant health-promoting rhizobacteria, plant parasitic nematodes and soil microorganisms. Meded. Faculteit Landbouwkundige Rijksuniversiteit Gent 53: 867–878.
Selvarajan R., Jeyarajan R. 1996. Inhibition of chickpea root-rot pathogens, Fusarium solani and Macrophomina phaseolina by antagonists. Indian J. Mycol. Plant Pathol. 26: 248–251.
Smith K.P., Handelsman J., Goodman R.M. 1999. Genetic basis in plants for interactions with disease-suppressive bacteria. Proc. Natl. Academy Sci. United States of America 96 (9): 4786–4790.
Steel R.G.D., Torrie J.H. 1980. Principles and Procedures of Statistics. New York, McGraw-Hill Book Company Inc., 481 pp.
Sunick K., Sungjoon Y., Honggi K. 1997. Selection of antagonistic bacteria for biological control of ginseng diseases. Korean J. Plant Pathol. 13: 342–348.
van Gundy S.D. 1984. Nematodes. p. 129–131. In: “Integrated Pest Management for Citrus”. Riverside: University of California, USA.
Wright B., Rowse H.R., Whipps J.M. 2003. Application of beneficial microorganisms to seeds during drum priming. Biocontrol Sci. Technol. 13 (6): 599–614.