RAPD-PCR of Trichoderma isolates and in vitro antagonism against Fusarium wilt pathogens of Psidium guajava L.
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Department of Biotechnology, FASC, MITS University, Lakshmangarh-332311, Sikar, Rajasthan, India
Molecular Plant Pathology Lab., CISH, Lucknow-227 017, India
Mahatma Jyotiba Phule Rohilkhand University, Bareilly, UP, India
Misra Ashok Kumar
Molecular Plant Pathology Lab., CISH, Lucknow-227 017, India
Journal of Plant Protection Research 2010;50(3):256–262
The paper concerns randomly amplified polymorphic DNA RAPD-PCR analysis of seven Trichoderma species isolates and their in vitro antagonism against wilt pathogens of Psidium guajava L. viz. Fusarium oxysporum f. sp. psidii (F. o. f. sp. psidii) and Fusarium solani . Out of 10 RAPD oligonucleotides (OPA 1–OPA 10) tested, seven markers OPA 1, 3, 5, 7, 8, 9 and 10 efficiently differentiated the isolates of Trichoderma and showed reproducible banding patterns. A total of 248 bands were obtained from these markers along with a 61.84% per cent similarity among the seven isolates of Trichoderma. From the seven isolates of Trichoderma, isolates T. harzianum, T. virens and T. viride were evaluated for in vitro efficacy against F. o. f. sp. psidii and F. solani. Per cent inhibition was maximum by direct use of Trichoderma spp. in dual cultures against both pathogens, F. o. f. sp. psidii and F. solani. Two species, T. virens and T. viride were superior in inhibiting the growth of both Fusarium spp. Fusarium isolates showed intra species variability.
The authors have declared that no conflict of interests exist.
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