ORIGINAL ARTICLE
Stress-tolerant antagonistic plant growth-promoting rhizobacteria from Zea mays
 
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Microbiology Department, School of Bioengineering and Biosciences, Lovely Professional University, Jalandhar, India
Submission date: 2017-11-11
Acceptance date: 2018-01-02
 
Journal of Plant Protection Research 2018;58(2):115–123
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ABSTRACT:
Biological control of plant diseases is strongly emerging as an effective alternative to the use of chemical pesticides and fungicides. In addition, stress tolerance is also an important attribute in the selection of bacteria for the development of microbial inoculants. Fourteen salt-tolerant bacteria showing different morphological features isolated from the rhizosphere of maize were evaluated for different plant growth-promoting activities. All isolates showed auxin production ranging from 5 to 24 µg/ml after 48 h incubation in tryptophan supplemented media. Phosphate solubilization ranged from 15 to 419 µg/ml. 1-aminocycloproprane-1-carboxylate (ACC)-deaminase activity was shown by 6 isolates, ammonia production by 9 isolates, siderophore production by 8 isolates while HCN production by 4 isolates. Four bacterial isolates showing all plant growth-promoting activities also showed strong antagonistic against Fusarium oxysporum, Fusarium verticillioides, Curvularia lunata and Alternaria alternata and abiotic stress tolerance against salinity, temperature, pH and calcium salts. Two select bacterial isolates significantly enhanced the growth of test plants pea and maize under greenhouse conditions. The bacterial isolate M1B2 showing highest growth promotion of test plants was identified as Bacillus sp. based on phenotypoic and 16S rRNA gene sequencing. The results indicated that the Bacillus sp. M1B2 is a potential candidate for the development of microbial inoculants for stressed environments.
CORRESPONDING AUTHOR:
Pratibha Vyas   
Microbiology Department, School of Bioengineering and Biosciences, Lovely Professional University, 144411 Jalandhar, India
 
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