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Susceptibility of Hypsipyla grandella (Lepidoptera: Pyralidae) to Bacillus thuringiensis strains
 
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1
Faculty of Agronomy and Veterinary Medicine, University of Brasília, Brazil
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Department of Plant Pathology, University of Brasília, Brazil
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Laboratory of Entomopathogenic Bacteria, Embrapa Genetic Resources and Biotechnology, Brazil
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Marcelo Tavares de Castro   

Faculty of Agronomy and Veterinary Medicine, University of Brasília, 70910-900, Brazil
Submission date: 2017-11-12
Acceptance date: 2018-01-23
 
Journal of Plant Protection Research 2018;58(1):102–105
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ABSTRACT
The use of Bacillus thuringiensis (Bt) to control insect pests is already established in various agronomic and forest crops, being a bacterium that has no polluting effect on the environment, is safe for mammals and vertebrates, lacks toxicity to plants and specifically targets insects. To date in-depth studies have not been conducted about the use of Bt to control the main pest of mahogany (Swietenia macrophylla King) and other Meliaceae species, the Hypsipyla grandella Zeller (Lepidoptera: Pyralidae). Therefore, this study aimed to test the pathogenicity of Bt strains on H. grandella caterpillars, as well determine the lethal concentration to kill 50% of the population (LC50) of the most promising strains. Ten strains of Bt toxic to lepidopteran proven in previous trials were used and these were incorporated into natural diet with mahogany seeds to check their mortality. The LC50 of the top five strains was determined. The results indicate that H. grandella is highly susceptible to Bt toxins and the S1905 strain is highly toxic. Therefore, the use of Bt strains may be a tool to be incorporated into the integrated management of this important pest.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (24)
1.
Agrofit. 2017. Phytosanitary Pesticides System. Available at: http://agrofit.agricultura.gov... [Accessed on: January 16, 2018].
 
2.
Baum J.A., Johnson T.B., Carlton B.C. 1999. Bacillus thuringiensis: natural and recombinant bioinsecticide products. Methods Biotechnology 5: 189–209. DOI: https://doi.org/10.1385/0-8960....
 
3.
Castro M.T., Montalvão S.C.L., Monnerat R.G. 2016. Breeding and biology of Hypsipyla grandella Zeller (Lepidoptera: Pyralidae) fed with mahogany seeds (Swietenia macrophylla King). Journal of Asia-Pacific Entomology 19 (1): 217–221. DOI: https://doi.org/10.1016/j.aspe....
 
4.
Castro M.T., Montalvão S.C.L., Souza D.A., Monnerat R.G. 2017. Ocorrência e patogenicidade de Beauveria bassiana à Hypsipyla grandella coletada em Brasília [Occurrence and pathogenicity of an isolate of Beauveria bassiana at Hyp-sipyla grandella collected in Brasilia, Brazil]. Nativa 5 (4): 263–266. DOI: https://doi.org/10.5935/2318-7....
 
5.
Finney D.J. 1971. Probit Analysis. Cambridge University Press, Cambridge, England, 31 pp.
 
6.
Goulet E., Rueda A., Shelton A. 2005. Management of the mahogany shoot borer, Hypsipyla grandella Zeller (Lepidoptera: Pyralidae), through weed management and insecticidal sprays in 1- and 2-years-old Swietenia humilis Zucc. plantations. Crop Protection 24 (9): 821–828. DOI: https://doi.org/10.1016/j.crop....
 
7.
Griffiths M.W. 2000. The biology and host relations of the red cedar tip moth, Hypsipyla robusta, in Australia. p. 135–140. In: “Opportunities for the New Millennium” (A. Snell, S. Vize, eds.). Proceedings of the Australian Forest Growers Biennal Conference. Canberra: Australian Forest Growers.
 
8.
Grijpma P. 1976. Resistance of Meliaceae against the shoot borer Hypsipyla with particular reference to Toona ciliata M. J. Roem. var. australis (F.v. Muell.) CDC. p. 69–78. In: “Tropical Trees: Variation Breeding and Conservation” (J. Burley, B.T. Styles, eds). London, Linnaean Society.
 
9.
Habib M.E.M., Andrade C.F.S. 1998. Bactérias entomopatogênicas [Entomopathogenic bacteria]. p. 383–446. In: “Controle Microbiano de Insetos” [“Microbial Control of Insects”] (S.B. Alves, ed.). FEALQ, Piracicaba.
 
10.
Hauxwell C., Vargas C., Opuni Frimpong E. 2001. Entomopathogens for control of Hypsipyla spp. p. 131–139. In: “Hypsipyla Shoot Borers in Meliaceae” (C. Hauxwell, R.B. Floyd, eds.). Proceedings of an International Workshop held at Kandy, Sri Lanka, 20–23 August 1996. Australian Centre for International Agricultural Research, Canberra, ACT, Australia.
 
11.
Hidalgo-Salvatierra O., Palm J.D. 1973. Susceptibility of first instar larvae to Bacillus thuringiensis. In: “Studies on the Shoot Borer Hypsipyla grandella (Zeller) Lep. Pyralidae” (P. Grijpma, ed.). IICA Biblioteca Venezuela, 88 pp.
 
12.
Lunz A.M., Thomazini M.J., Moraes M.C.B., Neves E.J.M., Batista T.F.C., Degenhardt J., Sousa L.A., Ohashi O.S. 2009. Hypsipyla grandella em Mogno (Swietenia macrophylla): situação atual e perspectivas [Hypsipyla grandella in Mahogany (Swietenia macrophylla): Current Situation and Perspectives]. Pesquisa Florestal Brasileira 59: 45–55. DOI: https://doi.org/10.4336/2009.p... (in Brazilian).
 
13.
Macedo C.L., Martins E.S., Macedo L.L.P., Santos A.C., Praça L.B., Góis L.A.B., Monnerat R.G. 2012. Seleção e caracterização de Bacillus thuringiensis eficientes contra a Diatraea saccharalis (Lepidoptera: Crambidae) [Selection and characterization of Bacillus thuringiensis efficient strains against Diatraea saccharalis (Lepidoptera: Crambidae)]. Pesquisa Agropecuária Brasileira 47 (12): 1759–1765. (in Brazilian).
 
14.
Mahroof R.M., Hauxwell C., Edirisinghe J.P., Watt A.D., Newton A.C. 2002. Effects of artificial shade on attack by the mahogany shoot borer, Hypsipyla robusta (Moore). Agricultural and Forest Entomology 4: 283–292. DOI: https://doi.org/10.1046/j.1461....
 
15.
Monnerat R.G., Batista A.C., Medeiros P.T., Martins E., Melatti V., Praça L.B., Dumas V., Demo C., Gomes A.C., Falcão R., Berry C. 2007. Characterization of Brazilian Bacillus thuringiensis strains active against Spodoptera frugiperda, Plutella xylostella and Anticarsia gemmatalis. Biological Control 41: 291–295. DOI: https://doi.org/10.1016/j.bioc....
 
16.
Ohashi O.S., Silva Junior M.L., Lameira O.A., Silva J.N.M., Leão N.V.M., Terezo E.F., Batista T.F.C., Hidaka D.Z.L., Almeida G.B., Bittencourt P.R.G., Gomes F.S., Neves G.A.M. 2005. Danos e controle da broca de Hypsipyla grandella em plantio de mogno no Estado do Pará [Damages and control of Hypsipyla grandella shoot borers on mahogany planting in Pará state]. In: “Pragas e Doenças de Cultivos Amazônicos” [“Pests and Diseases of Amazon Crops”] (L.S. Poltronieri, D.R. Trindade, I.P. Santos, eds). Belém, Embrapa Amazônia Oriental, 483 pp. (in Portuguese).
 
17.
Praça L.B., Gomes A.C.M.M., Cabral G., Martins E.S., Sujii E.H., Monnerat R.G. 2012. Endophytic colonization by brazilian strains of Bacillus thurigiensis on cabbage seedlings grown in vitro. Bt Research 3: 11–19. DOI: https://doi.org/10.5376/bt.201....
 
18.
Silva N.M. 1985. Características biológicas e demográficas de Hypsipyla grandella (Zeller, 1848) (Pyralidae, Lepidoptera) e níveis de infestação sob dois sistemas de plantio de Carapa guianensis Aubl. (Meliaceae) no Amazonas [Biological and demographic characteristics of Hypsipyla grandella (Zeller, 1848) (Pyralidae, Lepidoptera) and levels of infestation under two planting systems of Carapa guianensis Aubl. (Meliaceae) in the Amazon]. Dissertation, Departamento de Ciências Fundamentais e Desenvolvimento Agrícola, Instituto Nacional de Pesquisas da Amazônia, Brazil, 103 pp.
 
19.
Taveras R., Hilje L., Carballo M. 2004. Development of Hypsipyla grandella (Zeller) (Lepidoptera: Pyralidae) in response to constant temperatures. Neotropical Entomology 33 (1): 1–6. DOI: http://dx.doi.org/10.1590/S151....
 
20.
van Frankenhuyzen K. 1990. Development and current status of Bacillus thuringiensis for control of defoliating forest insects. Forest Chronicle 16 (5): 498–507. DOI: https://doi.org/10.5558/tfc664....
 
21.
Whiteley H.R., Schnepf H.E. 1986. The molecular biology of parasporal crystal body formation in Bacillus thuringiensis. Annual Review of Microbiology 40: 549–576. DOI: http://dx.doi.org/10.1146/annu....
 
22.
Wylie F.R. 2001. Control of Hypsipyla spp. shoot borers with chemical pesticides: a review. p. 109–117. In: “Hypsipyla Shoot Borers in Meliaceae” (R.B. Floyd, C. Hauxwell, ed.). Proceedings of an International Workshop. Australian Centre for International Agricultural Research. Canberra, ACT, Australia.
 
23.
Yamazaki S., Taketani A., Fujita K., Vasques C., Ikeda T. 1990. Ecology of Hypsipyla grandella and its seasonal changes in population density in Peruvian Amazon forest. Japan Agricultural Quarterly 24 (2): 149–155.
 
24.
Zanuncio J.C., Guedes R.N.C., Cruz A.P., Moreira A.M. 1992. Eficiência de Bacillus thuringiensis e de deltametrina, em aplicação aérea, para controle de Thyrinteina arnobia Stoll. 1782 (Lepidoptera: Geometridae) em eucaliptal no Pará [Efficiency of Bacillus thuringiensis and deltamethrin in aerial application to control Thyrinteina arnobia Stoll, 1782 (Lepidoptera: Geometridae) in eucalyptus in Pará]. Acta Amazônica 22 (4): 485–492. DOI: http://dx.doi.org/10.1590/1809... (in Brazilian).
 
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