Home
Print _CMN_EMAIL_ALT

 

In vitro evaluation of potato genotypes for resistance against bacterial soft rot (Pectobacterium carotovorum) – a new tool for studying disease resistance

Sima Azadmanesh1, Javad Mozafari2*, Nader Hasanzadeh1, Cobra Moslemkhani3

1Department of Plant Pathology, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 1477893855, Tehran, Iran

2Department of Genetics and National Plant Gene-Bank, Seed and Plant Improvement Institute, Agricultural Research Education and Extension Organization, Shahid Fahmideh Blvd., P.O. Box 4119, Karaj 31585, Iran

3Seed and Plant Certification Research Institute, Agricultural Research Education and Extension Organization, Nabovvat Blvd, P.O. Box 31535-1516, Karaj, Iran

 

*Corresponding address:

This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

 

Received: April 4, 2016

Accepted: January 23, 2017

 

Abstract

In vitro screening techniques were used to evaluate 46 genotypes of Iranian potato collection for resistance to bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc)One month old in vitro rooted potato plantlets were inoculated by two inoculation techniques under in vitro conditions: 1) sterile toothpicks dipped into bacterial suspension and pressed into the crown of plantlets and 2) the freshly cut crown of plantlets were dipped into bacterial suspension of 108 cfu ∙ ml–1 for 10 min. Typical soft rot disease symptoms, including the percentage of wilted leaves were recorded on inoculated plantlets 3, 6, 9, 12 and 15 days post-inoculation. The potato genotypes which were examined responded differently to Pcc and varying levels of resistance were observed. Potato genotype AG showed the highest level of resistance. Results obtained from in vitro screening were then verified by classical tuber slice assay. The verifications were conducted using five representative cultivars: Milva, Ramus, Picaso, Marfona and Agria which responded similarly to both in vitro and classical evaluation systems. Similar results obtained from these tests indicated that the in vitro screening technique developed in this study could provide a simple and rapid whole plant assay in selecting resistant potato genotypes against bacterial soft rot.

 

Key words: in vitro, potato soft rot, resistance, screening method

 

 
Editor in Chief
This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

Managing Editors
This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
Current issue cover
Who's Online
We have 1 guest online