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Topical treatment of LdMNPV-infected gypsy moth caterpillars with 18 nucleotides long antisense fragment from LdMNPV IAP3 gene triggers higher levels of apoptosis in infected cells and mortality of the pest

 

Volodymyr V. Oberemok1, Kateryna V. Laikova2, Alexey S. Zaitsev1, Palmah M. Nyadar1*, Yuri I. Gninenko3, Vladimir A. Gushchin4,5, Valentin V. Makarov6, Alexey A. Agranovsky4,7

 

1Department of Biochemistry, Taurida Academy of V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Crimea

2Department of Biochemistry, Medical Academy of V.I. Vernadsky Crimean Federal University, 295051 Simferopol, Crimea

3All-Russian Research Institute for Silviculture and Mechanization of Forestry, 141200 Pushkino, Russia

4Department of Virology, Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia

5Genetics Department of Russian Centre of Forest Health, 141207 Pushkino, Russia

6Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia

7Center of Bioengineering, Russian Academy of Sciences, 117312 Moscow, Russia

 

*Corresponding address:

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Received: September 28, 2016

Accepted: January 30, 2017

 

Abstract

The high efficiency of baculovirus infection is partially explained by the ability of the virus to suppress host defense machinery connected with the apoptosis pathway. Members of the baculovirus gene family, inhibitors of apoptosis (vIAPs), have been shown to inhibit apoptosis in baculovirus-infected cells. Here we showed that treatment of the LdMNPV-infected 1st instar gypsy moth (Lymantria dispar) caterpillars with sense (oligoBIR) and antisense (oligoRING) DNA oligonucleotides from the LdMNPV IAP3 gene induced elevated mortality of the insects. Apoptotic DNA ladder assay showed that the leading role in this phenomenon is played by the antisense oligoRING fragment of the vIAP3 gene. These results imply that the application of both antisense DNA oligonucleotides from vIAP genes and baculovirus preparations (one following the other) may be a potential method for plant protection against insect pests.

 

Key words: baculoviral infection, DNA insecticides, DNA oligonucleotides, forest and crop protection, gypsy moth, IAP genes

 

 

 
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