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Monitoring the occurrence of bacteria in stored cabbage heads

 

Aleš Eichmeier1, Eliška Peňázová2, Jakub Pečenka1, Jana Čechová1, Robert Pokluda2, Dorota Tekielska3*, Miroslav Baránek1

 

1Mendeleum – Institute of Genetics and Plant Breeding, Mendel University in Brno, Faculty of Horticulture, Valtická 337, 691 44 Lednice, Czech Republic

2Department of Vegetable Growing and Floriculture, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech Republic

3Department of Plant Protection, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Krakow, Poland

 

*Corresponding address:

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Received: December 5, 2016

Accepted: January 23, 2017

 

Abstract

Twenty-six cabbage heads stored under typical conditions in a storage hall in Moravia, Czech Republic, were tested for the presence of bacteria by the method of isolation from three different parts of the cabbage heads. Isolations were carried out from stalks, inner and superficial leaves. Two samplings were done; in November 2015 and February 2016. Bacterial cultures were sequenced in the part of 16S rRNA region; bacteria were identified according to the sequences obtained. The most prevalent bacteria were of the genus Pseudomonas. Genera: KlebsiellaErwinia, Pantoea, Bacillus were also identifiedThe results provided an interesting insight into the bacterial spectrum in stored cabbage heads and their dynamics during storage. The nucleotide sequences which were found were saved in GenBank/NCBI under accession numbers KX160104-KX160145.

 

Key words: Bacillus, bacteria, cabbage, identification, isolation, MPA medium, Pseudomonas, sequencing, 16S rRNA

 

 

 
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