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Effects of specific inhibitors on the gene expression of a digestive trypsin in Pieris brassicae L. (Lepidoptera: Pieridae)

 

Ali Sharifloo1, Arash Zibaee1*, Jalal Jalali Sendi1, Khalil Talebi Jahromi2

 

1Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

 

*Corresponding address:

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Received: September 23, 2017

Accepted: December 7, 2017

 

Abstract

Protein digestion in insects relies on several groups of proteases, among which trypsin plays a prominent role. In the current study, larvae of Pieris brassicae L. were fed radish leaves treated with 1 mM concentrations of three specific inhibitors of trypsin: AEBSF.HCl [4-(2- -aminoethyl)-benzenesulfonyl fluoride, monohydrochloride], TLCK (N-a-tosyl-l-lysine chloromethyl ketone) and SBTI (Soybean Trypsin Inhibitor) to find their potential effects on gene expression of trypsin. Initially, RT-PCR analysis revealed a gene of 748 bp responsible for synthesizing the digestive trypsin in P. brassicae larvae. Also, qRT-PCR data indicated a statistically greater expression of trypsin gene in the larvae fed 1 mM concentrations of AEBSF.HCl, TLCK and SBTI than the control. Results of the current study indicated that synthetic inhibitors can not only negatively affect the gene expression of P. brassicae trypsin, but also the insect can activate a compensatory mechanism against interruption of protein digestion by inducing more expression of the gene and producing more trypsin into the midgut lumen.

 

Key words: gene expression, Pieris brassicae, synthetic inhibitors, trypsin

 

 
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