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RAPID COMMUNICATION

 

A primary attempt of Leptinotarsa decemlineata control using contact DNA insecticide based on short antisense oligonucleotide of its CYP6B gene

 

Volodymyr Oberemok1, Kateryna Laikova2, Maksym Shumskykh1*, Igor Kenyo3,

Igor Kasich4, Karim Deri4, Eleonora Seidosmanova1, Alisa Krasnodubets1, Viktoriya Bekirova1, Nikita Gal’chinsky1

 

1Department of Biochemistry, Taurida Academy, V.I. Vernadsky Crimean Federal University, Republic of Crimea

2Department of Biochemistry, Medical Academy, V.I. Vernadsky Crimean Federal University, Republic of Crimea

3Department of Olericulture and Plant Protection, Academy of Bioresources and Environmental Management, V.I. Vernadsky Crimean Federal University, Republic of Crimea

4Department of Pathological Anatomy, Medical Academy, V.I. Vernadsky Crimean Federal University, Republic of Crimea

 

*Corresponding address:

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Received: December 13, 2017

Accepted: March 18, 2018

 

Abstract

Effective control of Leptinotarsa decemlineata remains an urgent problem for agriculture worldwide. Minimization of the use of non-selective neonicotinoid insecticides, such as thiomethoxam, is an actual vector of development of potato cultivation. In this rapid communication, we show the prospect of the topical use of short unmodified antisense fragment of L. decemlineata CYP6B gene as a DNA insecticide. Investigated parameters, namely, number of larvae per plant, aboveground biomass, yield and number of potatoes produced per plant indicate the possibility of this post-genomic approach as a safe and effective method of L. decemlineata control.

 

Key words: antisense oligonucleotide, cytochrome P450 monooxygenase gene, DNA insecticide, Leptinotarsa decemlineata, neonicotinoid insecticides, potato

 

 

 
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