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Characterization of Phyllosticta hostae causing Phyllosticta leaf spot on spider lily in China
Run Hua Yi 1,   Luo Jun Gan 1,   Jing Chen 1,   Xiao Ling Xu 1
 
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Department of Biotechnology, Agricultural College, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China
CORRESPONDING AUTHOR
Run Hua Yi
Department of Biotechnology, Agricultural College, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China
Submission date: 2015-05-05
Acceptance date: 2015-11-03
 
Journal of Plant Protection Research 2015;55(4):438–445
 
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ABSTRACT
Leaf spot disease on the spider lily [ Hymenocallis littoralis (Jacq.) Salisb.] continues to cause serious problems in China. To con- firm the pathogen, the pathogenicity of isolates from diseased leaves was tested according to Koch’s postulates. The isolates were tenta- tively identified using morphological characteristics and confirmation was done by phylogenetic analysis of the translation elongation factor 1-alpha gene (TEF1), the actin gene (ACT), and internal transcibed spacer (ITS) sequences using neighbor-joining (NJ), maximum parsimony (MP), and Bayesian inference (BI) methods. The pathogen was identified as Phyllosticta hostae . Molecular analysis indicated very little diversity in the TEF1, ACT, and ITS gene. This is the first report of P. hostae causing leaf spot disease on spider lily in China
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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