Characterization of a nucleopolyhedrovirus isolated from the labolatory rearing of the beet armyworm Spodoptera exigua (Hbn.) in Poland
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Institute of Plant Protection, Miczurina 20,60-318 Poznań
Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
Agata Jakubowska
Institute of Plant Protection, Miczurina 20,60-318 Poznań
Journal of Plant Protection Research 2005;45(4):279–286
A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-X R , was characterized molecularly and biologically. Phylogenetic analysis based on three conserved baculovirus genes, polh , lef-8 and pif-2 , showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb) MNPV and M. configurata(Maco) MNPV, and much less to SeMNPV (Spod-X R ).These findings were confirmed by genomic DNA restriction profile analyses. Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide Spod-X R was th emost inf ectious for S. exigua , while the infectivity of SeMNPV (P) and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a variant of MbMNPV
The authors have declared that no conflict of interests exist.
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