Chili (Capsicum annuum L.) genotypes resistant to Pepper yellow leaf curl Thailand virus (PepYLCTHV)
Patcharaporn Suwor 1, A-F  
,   Tawatchai Masirayanan 1, D,   Hathairat Khingkumpungk 1, B,   Somsak Kramchote 1, F,   Wen Shi Tsai 2, C,F,   Kanjana Saetiew 1, C,   Suchila Techawongstien 3, A,   Sanjeet Kumar 4, E-F
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Plant Production of Technology, King Mongkut’s Institute of Technology Ladkrabang, Thailand
Department of Plant Medicine,, College of Agriculture, National Chiayi University, Taiwan
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Thailand
Pepper breeding section, Plant Geneticist and Breeder (Independent),, India
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Patcharaporn Suwor   

Plant Production of Technology, King Mongkut’s Institute of Technology Ladkrabang, Faculty of Agricultural Technology, King Mongkut’s, 10520, Bangkok, Thailand
Submission date: 2021-03-26
Acceptance date: 2021-08-09
Online publication date: 2021-09-08
Pepper yellow leaf curl Thailand virus (PepYLCTHV) causes leaf curl disease in chili production regions of the tropics and subtropics. Information on PepYLCTHV disease severity and resistance in chili pepper is still limited in Thailand. This study reports PepYLCTHV disease severity through graft inoculation and selection of single resistant plants for use in a chili breeding program. Twenty-one chili genotypes consisting of the local cultivar (5) collected from Thailand, breeding lines (9) developed at Khon Kaen University (KKU), Thailand and improved lines (7) obtained from the World Vegetable Center, Taiwan were used in this study. Forty-five-day-old seedlings of all the genotypes were graft inoculated with PepYLCTHV in a randomized complete block design (RCBD) with three replications and 10 plants per replication and kept in a plastic net house. Disease symptoms were scored at 20, 27, 34, 41 48, and 55 days after graft/inoculation (DAI). Disease severity was visually recorded using 0-5 scores. Results showed that the disease severity of 21 chili genotypes significantly differed at 48 days after grafting. High resistance and stability were shown by 9853-123 genotypes. Two genotypes, PSP11-7 and PSP11-10-1, showed resistant reaction with disease severity scores of 1.9 and 1.8, respectively. However, among 21 chili genotypes or 630 grafted plants, 302 plants were successfully grafted inoculated plants. Therefore, from the results of this work, highly resistant plants (69 single plants) can be selected, selfed and advanced for breeding.
We wish to thank the Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand, for experimental facilities support and the World Vegetable Center (WorldVeg), Taiwan, for providing germplasm.
This research was supported by the Center of Excellence on Agricultural Biotechnology, Science and Technology, Postgraduate Education and Research Development Office, Office of Higher Education Commission, Ministry of Higher Education, Science, Research and Innovation (Grant # AG-BIO/PERDO-CHE) and Academic Melting Pot Fiscal Year, 2020 of King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand.
Przemysław Wieczorek
The authors have declared that no conflict of interests exist.