ORIGINAL ARTICLE
Differentiation by simplified AFLP of Pseudomonas syringe pv. syringae isolates from fields, panicles and nurseries of the Guilan Province - Iran
 
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1
University of Guilan, Rasht,41635-1314, Iran Department of Plant Protection, College of Agriculture
2
University of Guilan, Rasht,41635-1314, Iran Department of Plant Breeding, College of Agriculture Rasht
3
University of Guilan, Rasht,41635-1314, Iran Research Institute of Rice, Rasht, 41996-13475, Iran
CORRESPONDING AUTHOR
Roghayeh Habibi
University of Guilan, Rasht,41635-1314, Iran Department of Plant Protection, College of Agriculture
 
Journal of Plant Protection Research 2012;52(2):268–274
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ABSTRACT
Pseudomonas syringae pv . syringae is a plant pathogen bacterium in rice which causes bacterial sheath rot. In this study, simplified AFLP (Amplified fragment length polymorphism) was tested in order to evaluate genetic diversity of 38 Pseudomonas syringae pv . syringae strains isolated from rice fields of the Guilan province, by 12 AFLP primers. The products resulted from AFLP were separated using agarose gel electrophoresis. The maximum number of PIC among all tested primers belonged to primer 36. Based on the Unweighted Pair Group Method with Arithmetic (UPGMA) method, using NTSISpc Software, all Pseudomonas syringae pv . syringae strains were divided into three distinct clusters which had a 70% similarity level. Cluster analysis of studied populations (isolates of fields, panicles, and nurseries) determined that a high genetic identity could be seen among isolates obtained from the panicles and nurseries, while strains isolated from the fields and nurseries had the most genetic distance with each other. The result of this study showed that sampling site and weather conditions play an important role in genetic evolution of strains. It was also found that AFLP is an effective marker in evaluating genetic diversity within and among isolates being studied, while all of them had the same host and pathogenesis characteristics
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (24)
1.
Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G.G., Smith J.A., Struhl K. 1987. Current Protocols in Molecular Biology. Wiley, Inc., New York, 348 pp.
 
2.
Cirvilleri G., Bonaccorsi A., Scuder G., Scortichini M. 2005. Potential biological control activity and genetic diversity of Pseudomonas syringae strains. Phytopathology 153 (11–12): 654–666.
 
3.
Civilleri G., Scuderi G., Bonaccorsi A., Scortichini M. 2006. Molecular characterization of Pseudomonas syringae pv. Syringae strains from different host plants using fluorescent Amplified fragment length polymorphism. J. Plant Pathol. 88 (3): 329–333.
 
4.
Clerc A., Manceau C. Nesme X. 1998. Comparison of randomly amplified polymorphic DNA with amplified fragment length polymorphism to assess genetic diversity and genetic relatedness within genospecies III of Pseudomonas syringae. Appl. Environ. Microb. 64 (4): 1180–1187.
 
5.
Hildebrand D.C., Schroth M.N. Huisman O.C. 1982. The DNA homology matrix and non-random variation concepts as the basis for the taxonomic treatment of plant pathogenic and other bacteria. Annu. Rev. Phytopathol. 20 (1): 235–256.
 
6.
Jaimes C.P., Aristizabal G.F.A., Bernal M.M., Suarez Z.R., Montoya D. 2006. AFLP fingerprinting of Colombian Clostridium spp. strains, multivariate data analysis and its taxonomical implications. J. Microbiol. Methods 67 (1): 64–69.
 
7.
Kałużna M., Puławska J., Sobiczewski P. 2009. The use of PCR melting profile for typing of Pseudomonas syringae isolates from stone fruit trees. Eur. J. Plant Pathol. 126 (4): 437–443.
 
8.
Khoshkdaman M., Kazempour M.N., Ebadi A.A., Mossanejad E., Pedramfar H. 2008. Identification of causal agents of foot and sheath rot of rice in the field of Guilan province of Iran. Agric. Tropica et Subtropica 41 (1): 17–20.
 
9.
Kimura M. Crow J.F. 1964. The number of alleles that can be maintained in a finite population. Genetics 49 (4): 725–738.
 
10.
Klement Z. 1995. A new bacterial disease of rice caused by Pseudomonas oryzicola n. sp. Acta. Microbiol. Acad. Sci. Hung. 2 (3): 265–274.
 
11.
Kolliker R., Kraehenbuehl R., Boller B., Widmer F. 2006. Genetic diversity and pathogenecity of the grass pathogen Xanthomonas translucens pv. graminis. Syst. Appl. Microb. 29 (2): 109–119.
 
12.
Kosawang C., Smitamana R., Toojinda T., Nilpanit N., Sirithunya P. 2006. Amplified fragment length polymorphism fingerprinting differentiates genetic diversity of Xanthomonas Oryzae pv. Oryzae from Northern Thailand. J. Phytopathol. 154 (9): 550–555.
 
13.
Lewontin R.C. 1974. The Genetic Basis of Evolutionary Change. Columbia University Press, New York, USA, 346 pp.
 
14.
Little E.L., Bostock R.M., Kirkpatrick B.C. 1998. Genetic characterization of Pseudomonas syringae pv. Syringae starains from stone fruit in California. Appl. Environ. Microb. 64 (10): 3818–3823.
 
15.
Maurilio L.M., Pinto C.L.O., Rocha R.B., Araujo de E.F., Vanetti M.C.D. 2006. Genetic diversity of Gram-negative, proteolytic, psychrotrophic bacteria isolated from refrigerated raw milk. Int. J. Food. Microbiol. 111 (2): 144–148.
 
16.
Natalini E., Rossi M.P., Barionovi D., Scortichini M. 2006. Genetic and pathogenic diversity of Pseudomonas syringae pv. syringae isolates associated with bud necrosis and leaf spot of pear in a single orchard. J. Plant Pathol. 88 (2): 219–223.
 
17.
Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. 70 (12): 3321–3323.
 
18.
Ochiai H., Horino O., Miyajima K., Kaku H. 2000. Genetic diversity of Xanthomonas oryzae pv. Oryzae strains from Sri Lanka. Phytopathology 90 (4): 415–421.
 
19.
Restrepo S., Duque M., Tohme J., Verdier V. 1999. AFLP fingerprinting: An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis. Microbiology 145 (1): 107–114.
 
20.
Scortichini M. 2005. The population structure of some plant pathogenic bacteria: An ecological and adaptive perspective. J. Plant Pathol. 87 (1): 5–12.
 
21.
Sisto A., Cipriani M.G., Tegli S., Cerboneschi M., Stea G., Santilli E. 2007. Genetic characterization by fluorescent AFLP of Pseudomonas savastanoi pv. savastanoi strains isolated from different host species. Plant Pathol. 56 (3): 366–372.
 
22.
Slawiak M., Przysowa J., Szymanowska J., Nesme X., Lojkowska E. 2005. Application of PCR-RFLP and simplified AFLP for differentiation of bacteria from the Pseudomonas species. Phytopath. Pol. 35: 85–93.
 
23.
Tae Soon K., Hung Goo W., Chan Sun C. 2008. Importance of rice genetic resources and current status of rice breeding and cultivation in Korea. J. Fac. Agric. Shinshu Univ. 44 (1–2): 35–37.
 
24.
Yu Q., Alvarez A.M., Moore P.H., Zee F., Kim M.S., Silva de A., Hepperly P.R., Ming R. 2003. Molecular diversity of Ralstonia solanacearum isolated from ginger in Hawaii. Phytopathology 93 (9): 1124–1130.
 
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