ORIGINAL ARTICLE
Genetic diversity among Xanthomonas citri subsp. citri strains in Iran
 
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1
Plant Pathology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2
Iranian Research Institute of Plant Protection, Tehran, Iran
CORRESPONDING AUTHOR
Masoud Shams-Bakhsh
Plant Pathology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
 
Journal of Plant Protection Research 2012;52(1):1–9
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ABSTRACT
Citrus bacterial canker (CBC) is one of the most important diseases of citrus. It is caused by Xanthomonas citri subsp. citri ( Xcc ). To investigate the variability of Xcc , a collection of twenty-five strains were isolated from the Fars, Hormozgan, Kerman and Sistan-va-Baluchestan provinces of Iran. The twenty-five strains were assessed phenotypically and genetically. These strains had simi - lar biochemical properties. Based on host range determination, the strains were divided into two groups; the first group was patho- genic on Mexican lime ( Citrus aurantifolia ), citrumelo ( Poncirus trifoliata x C . paradisi ), citrange ( C . sinensis x P . trifoliata ) and sour orange ( C . aurantium ) varieties. The second group was pathogenic on Mexican lime only. Profile of cellular soluble proteins analyzed by sodium dodecyl sulphate-polyacryamid gel electrophoresis (SDS-PAGE) did not reveal any considerable differences among strains. Genetic diversity analyses were performed using two marker systems; repetitive polymerase chain reaction (rep-PCR) and random amplified polymorphic DNA (RAPD). The results of this research showed that two primers, ERIC 1R and 232, with the highest marker index, resulted in the most genetic variability among strains. Cluster analysis by band patterns showed that strains from the Sistan-va- Baluchestan province were a different group, so it was concluded that geographical origin of strains from the Sistan-va-Baluchestan province is different than the geographical origin of strains isolated from other provinces.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (45)
1.
Albufera U., Bhugaloo-Vial P., Issack M.I., Jaufeerally-Fakim Y. 2009. Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis. Infect. Genet. Evol. 9 (3): 322–327.
 
2.
Alizadeh A., Rahimian H. 1990. Citrus canker in Kerman province. Iranian J. Plant Pathol. 26, p. 118.
 
3.
Al-Saadi A., Reddy J.D., Duan Y.P., Bruning A.M., Yuan Q., Gabriel D.W. 2007. All five host-range variants of Xanthomonas Citri carry one pthAhomolog with 17.5 repeats that determines pathogenicity on citrus, but not host-range variation. Mol. Plant Microbe Interaction 20 (8): 934–943.
 
4.
Bruning A.H., Gabriel D.W. 2003. Xanthomonas citri: breking the surface. Mol. Plant Pathol. 4 (3): 141–157.
 
5.
Buithingoc L.B., Verniere C., Vital K., Guerin F., Gagnevin L., Brisse S., Ah-You N., Pruvost O. 2009. Development of 14 minisatellite markers for the citrus canker bacterium, Xanthomonas Citri subsp. citri. Mol. Ecol. Res. 9: 125–127.
 
6.
Chadha S., Gopalakrishna T. 2007. Comparative assessment of REMAP and ISSR marker assays for genetic polymorphism studies in Magnaporthe grisea. Current Sci. 93 (5): 688–692.
 
7.
Civerolo E.L. 1984. Bacterial canker disease of citrus. J. Rio Grande Valley Hortic. Soc. 37: 127–146.
 
8.
Coletta-Filho H.D., Takita M.A., Souza A.A., Neto J.R., Destefano S.A.L., Hartung J.S., Machado M.A. 2005. Primers based on the rpfgene region provide improved detection of Xanthomonas axonopodis pv. citri in naturally and artificially infected citrus plants. J. Appl. Microbiol. 100 (2): 279–285.
 
9.
Cubero J. Graham J.H. 2002. Genetic relationship among world wide strains of Xanthomonas causig canker in citrus species and design of new primers for their identification by PCR. Appl. Environ. Microbiol. 68 (3): 1256–1264.
 
10.
Dye D.W., Bradbury J.F., Goto M., Hayward A.C., Lelliot R.A., Schroth M.N. 1980. International standards for naming pathovars of phytopathogenic bacteria and a list of pathovar names and pathotype strains. Rev. Plant Pathol. 59 (4): 153–168.
 
11.
Egel D.S., Graham J.H., Stall R.E. 1991. Genomic relatedness of Xanthomonas campestris strains causing diseases of citrus. Appl. Environ. Microbiol. 57 (9): 2724–2730.
 
12.
Fahy P.C. Persley G.J. 1983. Plant Bacterial Disease. A Diagnostic Guide. Academic Press, Syndey, 393 pp.
 
13.
Gottwald T.R., Graham J.H., Civerolo E.L., Barrett H.C., Hearn C.J. 1993. Differential host range reaction of citrus and citrus relatives to citrus canker and citrus bacterial spot determined by leaf mesophyll susceptibility. Plant Dis. 77 (10): 1004–1009.
 
14.
Graham J.H., Hartung J.S., Stall R.E., Chase A.R. 1990. Pathological, restriction-fragment length polymorphism, and fatty acid profile relationship between Xanthomonas campestrisfrom citrus and noncitrus hosts. Phytopathology 80 (9): 829–836.
 
15.
Hartung J.S. 1992. Plasmid-based hybridization probes for detection and identification of Xanthomonas campestrisb pv. citri. Plant Dis. 76 (9): 889–893.
 
16.
Hasse C.H. 1915. Pseudomonas citri, the cause of citrus canker. J. Agric. Res. 4 (1): 97–100.
 
17.
Hu J., Zhang Y., Qian W., He C. 2007. Avirulence gene and insertion element based RFLP as well as RAPD markers reveal high levels of genomic polymorphism in the rice pathogen Xanthomonas oryzae pv. oryzae. Syst. Appl. Microbiol. 30 (8): 587–560.
 
18.
Khodakaramian G., Swings J. 2002. AFLP fingerprinting of the strains of Xanthomonas axonopodis including citrus canker disease in southern Iran. J. Phytopathol. 150 (4): 227–231.
 
19.
Khodakaramian G., Rahimian H., Mohamadi M., Alameh A. 1999. Phenotypic features, host range and distribution of the strains Xanthomonasn axonopodis including citrus canker in southern Iran. Iranian J. Plant Pathol. 35: 102–111.
 
20.
Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature 227 (5259): 680–685.
 
21.
Lee Y.H., Lee S., Lee D.H., Yu S.M., Lee J., Heu S., Hyum J.W.,.Ra D.S. Park E.W. 2008. Difference of physiochemical characteristics between citrus bacterial canker pathotypes and identification isolates with repetitive sequence PCRs. Plant Pathol. J. 24 (4): 423–432.
 
22.
Louws F.J., Fulbright D.W., Stephens C.T. de Bruijn F.J. 1994. Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strain generated with repetitive sequences and PCR. Appl. Environ. Microbiol. 60 (7): 2286–2295.
 
23.
Louws F.J., Fulbright D.W., Stephens C.T. de Bruijn F.J. 1995. Differentiation of genomic structure by rep-PCR fingerprinting to rapidally classify Xanthomonas campestris pv. vesicatoria. Phytopathology 85 (5): 528–536.
 
24.
Mohammadi M., Mirzaee M.R., Rahimian H. 2001. Physiological and biochemical characteristics of Iranian strains of Xanthomonas axonopodis pv. citri, the casual agent of citrus bacterial canker disease. J. Phytopathol. 149 (2): 65–75.
 
25.
Mohammadi S.A., Prasanna B.M. 2003. Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Sci. 43 (3): 1235–1248.
 
26.
Opgenorth D.C., Smart C.D., Louws F.J., de Bruijin F.J., Kirkpatrick B.C. 1996. Identification of Xanthomonas fragariae filed isolates by rep-PCR genomic fingerprinting. Plant Dis. 80 (8): 868–873.
 
27.
Powell W., Morgante M., Andre C., Vogel J., Tingey S. Rafalski A.The comparison of RFLP, RAPD, AFLP and SSR (microsattlite) markers for germplasm analysis. Mol. Breed. 2 (3): 225–238.
 
28.
Pruvost O., Hartung J.S., Civerolo E.L., Dubios C. Perrier X. 1992. Plasmid DNA fingerprinst distinguish pathotypes of Xanthomonas campestris pv. citri, the casual agent of citrus bacterial canker disease. Phtopathology 82 (4): 485–490.
 
29.
Rademarker J.L.W., de Bruijn F.J. 1997. Chracterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. p. 151–157. In: “DNA Markers: Protocols, Application and Overviews” (G. Caetano, A. Nolles, P.H. Gresshoff, eds.). Diley, Liss, 364 pp.
 
30.
Rohlf F.J. 2000. NTSYSpc: Numerical Taxonomy System, ver. 2.1. New York, Exeter Publishing.
 
31.
Schaad N.W., Jones J.B. Chun W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. APS, St. Paul, MN, USA, 373 pp.
 
32.
Schaad, N.W., Postnikova E., Lacy G., Sechler A., Agarkova I., Stormberg P.E., Stormberg V.K. Vidaver A.K. 2005. Reclas sification of Xanthomonas campestris pv. Citri (ex Hasse 1915) Dye1978 forms A, B/C/D, and E as X. smithii subsp. citri (ex Hasse) sp. nov.nom. rev. comb. nov., X. fuscanssubsp. Aurantifolii (ex Gabriel 1989) sp. nov. nom. rev. comb. nov., and X. alfalfae subsp. citrumelo (ex Riker andJones) Gabriel et al., 1989 sp. nov. nom. rev.comb. nov.; X. campestris pv. Malvacearum (ex Smith 1901) Dye 1978 as X. smithii subsp. smithii nov. comb. nov. nom. nov.; X. campestris pv. Alfalfae (ex Riker and Jones,1935) Dye 1978 as X. alfalfae subsp. Alfalfae (ex Riker et al., 1935) sp.nov. nom. rev.; and ‘‘var. fuscans’’ of X.Campestris pv. Phaseoli (ex Smith, 1987) Dye 1978 as X. fuscans subsp. Fuscans sp. nov. Syst. Appl. Microbiol. 28 (6): 494–518.
 
33.
Schaad N.W., Postnikova E., Lacy G., Sechler A., Agarkova I., Stormberg P.E., Stormberg V.K. Vidaver A.K. 2006. Emendd classification of xanthomonad pathogens on citrus. Syst. Appl. Microbiol. 29 (8): 690–695.
 
34.
Stall R.E., Civerolo. 1991. Research relating to the recent outbreaks of citrus canker in Florida. Annu. Rev. Phytopathol. 29: 399–420.
 
35.
Sun X., Stall R.E., Jones J.B., Cubero J., Gottwald T.R., Graham J.H., Dixon W.N., Schubert T.S., Chaloux P.H., Stromberg V.K., Lacy G.H. Sutton B.D. 2004. Detection and characterization of a new strain of citrus canker bacteria from Key/Mexican lime and alemow in South Florida. Plant Dis. 88 (11): 1179–1188.
 
36.
Trebaol G., Manceau C., Tirilly Y., Boury S. 2001. Assessment of the genetic diversity among strains of XanthomonasCynarae by randomly amplified polymorphic DNA analysis and development of specific characterized amplified regions for the rapid identification of X. cynarae. Appl. Environ. Microbiol. 67 (8): 3379–3384.
 
37.
Vauterin L., Yang P., Hoste B., Vancanneyt M., Civerolo E.L., Swings J. Kersters K. 1991. Differentiation of Xanthomonas Campestris pv. Citri strains by sodium dodecyl sulfate-polyacrylamide gel electrophresis of proteins, fatty acid analysis, and DNA-DNA hybridization. Int. J. Syst. Bacteriol. 41 (4): 535–542.
 
38.
Vauterin L., Hoste B., Kersters K. Swings J. 1995. Reclassification of Xanthomonas. International. J. Syst. Bacteriol. 45 (3): 472–489.
 
39.
Vernière C., Hartung J.S., Pruvost O.P., Civerolo E.L., Alvarez A.M., Maestri P. Luisetti J.1998. Characterization of phenotypically distinct strains of Xanthomonas axonopodis pv. Citri from Southwest Asia. Eur. J. Plant Pathol. 104 (5): 477–487.
 
40.
Versalovic J., Koeuth T., Lupski J.R. 1991. Distribution of repetitive DNA sequences in Eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acid Res. 19 (24): 6823–6831.
 
41.
Versalovic J., Schneider M., de Bruijn F.J., Lupski J.R. 1994. Genomic fingerprinting of bacteria using repetitive sequence based PCR (rep-PCR). Methods Cell Mol. Biol. 5 (1): 25–40.
 
42.
Welsh J., McClelland M. 1990. Fingerprinting genome using PCR with arbitrary primers. Nucleic Acid Res. 18 (24): 7213–7218.
 
43.
Williams J.G., Kubelik A.R., Livak K.J., Rafalski J.A. Tingey S.V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res. 18 (22): 6531–6535.
 
44.
Witeside J.O., Gransey L.W., Timmer L.W. 1993. Compendium of Citrus Disease. APS, St. Paul, MN, USA, 80 pp.
 
45.
Young J.M., Dye D.W., Bradbury J.F., Panagopoulos C.G., Robbs C.F. 1978. A proposed nomenclature and classification for plant pathogenic bacteria. New Zealand J. Agric. Res. 21: 153–177.
 
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