Genetic polymorphism of Fusarium culmorum isolates originating from roots and stem bases of barley
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Poznań University of Life Sciences, Department of Phytopathology Dąbrowskiego 159, 60-594 Poznań, Poland
University of Technology and Life Sciences, Department of Phytopathology Kordeckiego 20, 85-225 Bydgoszcz, Poland
Lidia Irzykowska
Poznań University of Life Sciences, Department of Phytopathology Dąbrowskiego 159, 60-594 Poznań, Poland
Journal of Plant Protection Research 2008;48(3):303–312
Fusarium culmorum is an etiologic agent of barley foot rot. The identification and variability evaluation of F. culmorum isolates, originating from roots and stem bases of spring barley, was carried out using molecular methods. Species-specific SCAR primers were successfully applied to identify F. culmorum isolates from northern and south-eastern Poland. To determine DNA polymorphism on intraspecies level RAPD technique was used. Twenty three RAPD markers revealed DNA polymorphism suitable to assess genetic variation among isolates examined. Cluster analysis of RAPD data identified a few groups of isolates. In some cases grouping of isolates was correlated with their geographic origin.
The authors have declared that no conflict of interests exist.
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