ORIGINAL ARTICLE
Identification of Pch1 eyespot resistance gene in the collection of wheat lines (Triticum aestivum L.)
| 1 | Institute of Plant Genetics Polish Academy of Science
Strzeszyńska 34, 60-479 Poznań, Poland |
| 2 | Institute of Plant Protection – National Research Institute
Władysława Węgorka 20, 60-318 Poznań, Poland |
CORRESPONDING AUTHOR
Michał Kwiatek
Institute of Plant Genetics Polish Academy of Science Strzeszyńska 34, 60-479 Poznań, Poland
Institute of Plant Genetics Polish Academy of Science Strzeszyńska 34, 60-479 Poznań, Poland
Journal of Plant Protection Research 2012;52(2):254–258
KEYWORDS
TOPICS
ABSTRACT
Endopeptidase marker
EpD1b
and STS marker
XustSSR2001-7DL
are closely linked to very effective eyespot resistance gene
Pch1
. Because of this, the aim of this study was to compare the results obtained under lab conditions using such markers with the
results obtained under field conditions. 134 wheat breeding lines and
Triticum aestivum
L. var. Randevous used as a eyespot resistance
control were analized. The combination of three methods allowed to select eight completely resistant or high resistant lines, that
could be used in following breeding processes. Results obtained using endopeptidase and STS markers in 100% correlate with the
phenotyping scoring.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (22)
1.
Batts C.C.V., Fiddian W.E.H. 1955. Effect of previous cropping on eyespot in four varieties of winter wheat. Plant Pathol.4: 25–28.
2.
Chapman N.H., Burt C., Dong H., Nicholson P. 2008. The development of PCR-based markers for the selection of eyespot resistance genes Pch1 and Pch2. Theor. Appl. Genet. 117: 425–433.
3.
Crous P.W., Groenewald J.Z.E., Gams W. 2003. Eyespot of cereals revisited Its phylogeny reveals new species relationships. Eur. J. Plant Pathol. 109: 841–850.
4.
Doohan F.M., Parry D.W., Jenkinson P., Nicholson P. 1998. The use of species-specific PCR-based assays to analyse Fusarium ear blight of wheat. Plant Pathol. 47: 197–205.
5.
Fitt B.D.L., Goulds A., Pollet R.W. 1988. Eyespot (Pseudocercosporella herpotrichoides) epidemiology in relation to prediction of disease severity and yield loss in winter wheat; a review. Plant Pathol. 37: 311–328.
6.
Groenewald J.Z., Marais A.S., Marais G.F. 2003. Amplified fragmentlength polymorphism-derived micro-satellite sequence linked to the Pch1 and Ep-D1loci in common wheat. Plant Breed. 122: 83–85.
7.
Hart G.E., Langston P.J. 1977. Chromosomal location and evolution of isozyme structural genes in hexaploid wheat. Heredity 39: 263–277.
8.
Hollins T.W., Lockley K.D., Blackman J.A., Scott P.R., Bingham J. 1988. Field performance of Rendezvous, a wheat cultivar with resistance to eyespot (Pseudocercosporella herpotrichoides) derived from Aegilops ventricosa. Plant Pathol. 37: 251–260.
9.
Jahier J., Doussinault G., Dosba F., Bourgeois F. 1978. Monosomic analysis of resistance to eyespot in the variety “Roazon”. p. 437–440. In: Proc. 5th International Wheat Genet. Symposium (S. Ramamujan, ed.). 23–28, New Dehli, India, February 1978. 510 pp.
10.
Janczewska A. 1991. Yield loss of winter wheat as influenced by the intensity of Pseudocercosporella herpotrichoides (Fron.) attack. Phytopathol. Pol. 1 (XIII): 41–44.
11.
Koebner R.M.D., Miller T.E., Snape J.W., Law C.N. 1988. Wheat endopeptidase: genetic control, polymorphism, intrachromosomal gene location, and alien variation. Genome 30: 186–192.
13.
Law C.N., Scott P.R., Worland A.J., Hollins T.W. 1975. The inheritance of resistance to eyespot Cercosporella herpotrichoides.in wheat. Genet. Res. 25: 73–79.
14.
Maia N. 1967. Obtention de blés tendres résistants au piétinverse par croisements interspécifiques blés × Aegilops. C.R. Acad. Agric. Fr. 53: 149–154.
15.
McIntosh R.A., Hart G.E.,Devos K.M., Gale M.D., Rogers W.J. 2006. Catalogue of Gene Symbols for Wheat.http://www.shigen.nig.ac.jp/wh....
16.
McMillin D.E., Tuleen N.A. 1977. Determination of the linkage relationships and the gene centromere genetic distances for endopeptidase structural genes in hexaploid wheat. Genetics 86 (Suppl.), p. 44.
17.
McMillin D.E., Allan R.E., Roberts D.E. 1986. Association of an isozyme locus and strawbreaker foot rot resistance derived from Aegilops ventricosa in wheat. Theor. Appl. Genet. 72: 743–747.
18.
Mena M., Doussinault G., Lopez-Brana I., Aguaded S., Garcia-Olmedo F., Delibes A. 1992. Eyespot resistance gene Pch-1. H-93 wheat lines. Evidence of linkage to markers of chromosome group 7 and resolution from the endopeptidase locus Ep-D1b. Theor. Appl. Genet. 83: 1044–1047.
19.
Murray T.D. 1992. Pseudocercosporella. p. 149–152. In: “Methods for Research on Soilborne Phytopathogenic Fungi” (L.L. Singleton, J.D. Mihail, C.M. Rush, eds). APS Press, St. Paul, MN, USA, 265 pp.
20.
Santra D.K., Watt C., Little L., Kidwell K.K., Campbell K.G. 2006. Comparison of a modified assay method for the endopeptidase marker Ep-D1bwith the Sequence Tag Site marker XustSSR2001–7DL for strawbreaker foot rot resistance in wheat. Plant Breed. 125: 13–18.
21.
Vincent A., Ponchet J., Koller J. 1952. Recherche de varietes de bles tendres peu sensibles au pietin-verse: resullats preliminaires. [Research on wheat varieties insusceptible to eyespot: Preliminary results]. Ann. de I’Amelioration des Plantem 3: 459–472.
22.
Worland A.J., Law C.N., Hollins T.W., Koebner R.M.D., Giura A. 1988. Location of a gene for resistance to eyespot (Pseudocercosporella herpotrichoides) on chromosome 7D of bread wheat. Plant Breed. 101: 43–51.
RELATED ARTICLE
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.