Molecular characterization of the partial coat protein gene of an Onion yellow dwarf virus isolate detected in garlic (Allium sativum L.) from the West Shewa zone of Ethiopia
Yohanis Kebede 1, 2, B,D,   Jyoti Singh 1, B,   Shahana Majumder 1, 3, A,C,E-F  
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Department of Biotechnology, School of Engineering and Technology, Sharda University, Knowledge Park III, Greater Noida, India
Department of Plant Sciences, College of Agriculture and Veterinary Science, Ambo University, Ambo, Ethiopia
Department of Botany, School of Life Sciences, Mahatma Gandhi Central University, Chanaky Parisar, Motihari, District East Champaran, Bihar, India
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Shahana Majumder   

Department of Biotechnology, School of Engineering and Technology, Sharda University, 32-34, Knowledge Park III,, 201306, Greater Noida, Gautam Budh Nagar, India
Submission date: 2019-05-14
Acceptance date: 2019-09-02
Online publication date: 2020-03-09
Journal of Plant Protection Research 2020;60(1):106–111
Onion yellow dwarf virus (OYDV), an aphid-borne potyvirus is one of the major viral pathogens of garlic causing significant yield losses worldwide. It is found almost everywhere in the world where Allium species is grown. The aim of this study was to test the presence of OYDV infection in garlic from Ethiopia. The presence of the virus was tested by Reverse transcription polymerase chain reaction (RT-PCR). The direct sequencing of the PCR product produced a sequence of 296 bp. Sequence analysis showed 89.27% sequence homology with an isolate from Australia (HQ258894) and 89.29% with an isolate from Spain (JX429964). A phylogenetic tree constructed with MEGA 7.0 revealed high levels of homology with various isolates of OYDV from all over the world and thus further confirmed the identity of the virus.
The authors have declared that no conflict of interests exist.
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