RAPID COMMUNICATION
Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew
| 1 | Department of Studies in Microbiology, Karnataka State Open University, Mukthagangotri, Mysuru, Karnataka, India |
| 2 | Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka, India |
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Niranjan-Raj Sathyanarayana
Department of Studies in Microbiology, Karnataka State Open University, Mukthagangotri, Mysuru, Karnataka, India
Department of Studies in Microbiology, Karnataka State Open University, Mukthagangotri, Mysuru, Karnataka, India
Online publish date: 2019-10-02
Submission date: 2018-11-17
Acceptance date: 2019-08-22
Journal of Plant Protection Research 2019;59(3):423–427
KEYWORDS
TOPICS
ABSTRACT
Polyphenol oxidase partial gene PG-PPO was cloned and characterized from Pennisetum
glaucum (pearl millet) which showed 42% identity to a PPO sequence isolated from wheat
at the region of Copper B with a score of 40 and e-value of 2.8. Multiple sequence alignment
results revealed similarity to polyphenol oxidase (PPO) sequences from wheat, trifolium,
lettuce, apricot, tobacco, tomato, pokeweed, apple, grape and poplar especially at the
Copper B region of PPO. The 395 bp pearl millet PPO sequence was AT rich (53.3%) and
contained the highly conserved amino acids of histidine-rich copper binding sites similar
to PPO sequences from other crops. Results also indicated that PPO in pearl millet exists
in multi copy. The role of the isolated PPO gene during pearl millet-downy mildew interaction
was analyzed and the results showed significantly higher and rapid accumulation of
PPO mRNAs in resistant pearl millet seedlings inoculated with Sclerospora graminicola in
comparison to the susceptible control, demonstrating that the PPO plays a prominent role
in pearl millet defense against pathogens, particularly downy mildew pathogen.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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