RAPID COMMUNICATION
The molecular identification and phylogeny of moths feeding on cereals, belonging to Cnephasia species based on cytochrome c oxidase subunit I gene
Marta Budziszewska 1, A-F  
,   Wojciech Kubasik 2, B,D
 
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1
Department of Molecular Biology and Biotechnology, Institute of Plant Protection – National Research Institute, Poznań, Poland
2
Department of Monitoring and Signalling of Agrophages, Institute of Plant Protection – National Research Institute, Poznań, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Marta Budziszewska   

Department of Molecular Biology and Biotechnology, Institute of Plant Protection – National Research Institute, W. Wegorka 20,, 60-318, Poznan, Poland
Submission date: 2020-06-16
Acceptance date: 2020-08-10
Online publication date: 2020-11-10
 
Journal of Plant Protection Research 2020;60(4):410–414
 
KEYWORDS
TOPICS
ABSTRACT
The genus Cnephasia is represented by more than 70 species of insects worldwide, including serious pests of agricultural plants, mainly cereals. Since members of this genus are frequently very similar externally, species determination based on morphotaxonomy is time-consuming and difficult, especially for non-taxonomists. Hence, it could possibly be replaced by molecular biology approaches. A short nucleotide sequence of mitochondrial cytochrome oxidase I (mtCOI) constitutes a commonly used molecular marker for phylogenetic analyses identification. The aim of this work was molecular species determination of leaf rollers, collected in Poland, that on the basis of external features were hardly distinguishable. We amplified, sequenced and phylogenetically studied the fragment of the mtCOI gene for each individual. Comparative analyses showed the highest nucleotide similarity to C. genitalana, C. longana, C. pasiuana, C. asseclana and C. stephensiana, which was also confirmed by phylogeny. Obtained results showed genetic variation of the analyzed fragment of the mtCOI gene between analyzed Cnephasia spp. found in Poland that can be helpful in proper species determination. This in turn, may be essential for successful biological control and pest monitoring in crop cultivation.
ACKNOWLEDGEMENTS
We wish to thank Dr. A. Obrępalska-Stęplowska from IPP-INR for her valuable help and suggestions during the preparation of the manuscript.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
FUNDING
This work was supported by the Polish Ministry of Science and Higher Education, statutory activity project: BIOTECH1.
 
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