ORIGINAL ARTICLE
Detection of barley- and wheat-specific forms of Wheat Dwarf Virus in it's vector Psammotettix alienus by duplex PCR assay
| 1 | Department of Virology and Bacteriology, Institute of Plant Protection − National Research Institute, Władysława Węgorka 20, Poznań, Poland |
| 2 | Department of Entomology, Institute of Plant Protection − National Research Institute, Władysława Węgorka 20, Poznań, Poland |
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Katarzyna Trzmiel
Department of Virology and Bacteriology, Institute of Plant Protection − National Research Institute, Władysława Węgorka 20, Poznań, Poland
Department of Virology and Bacteriology, Institute of Plant Protection − National Research Institute, Władysława Węgorka 20, Poznań, Poland
Submission date: 2017-10-06
Acceptance date: 2017-12-18
Journal of Plant Protection Research 2018;58(1):54–57
KEYWORDS
TOPICS
ABSTRACT
Wheat dwarf virus (WDV) has been one of the most common viruses on cereal crops in Poland, in the last years. This ss DNA virus is transmitted by leafhopper, Psammotettix alienus (Dahlb.) in persistent manner. It induces yellowing and streaking of leaves, dwarfing or even death of infected plants. The presence of barley- and wheat- specific forms of WDV (WDV-B and WDV-W) and its vector was previously reported in the country, however the literature data did not include any information on the vectors infectivity in Poland. A duplex polymerase chain reaction (PCR) procedure was developed and optimized for simultaneous detection and differentiation of both WDV forms in its vector. Two sets of primers amplify 734 bp and 483 bp specific fragments for WDV-W and WDV-B, respectively. The results were verified by sequencing method. The studies were carried out on a set of insect samples collected in autumn from four different locations in Greater Poland. The results confirmed the presence of WDV-W in tested samples as well as suggested the possibility of two forms of the virus in the vector. Additional studies to determine virus-vector relationships should be undertaken.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (24)
1.
Commandeur U., Huth W. 1999. Differentiation of strain of wheat dwarf virus in infected wheat and barley plants by means of polymerase chain reaction. Journal of Plant Diseases and Protection 106 (5): 550–552.
2.
Ekzayez A.M., Kumari S.G., Ismail I. 2011. First report of Wheat dwarf virus and its vector (Psammotettix provincialis) affecting wheat and barley crops in Syria. Plant Disease 95 (1): 76. DOI: https://doi.org/10.1094/PDIS-0....
3.
Gadiou S., Ripl J., Jaňourová B., Jarošová J., Kundu J.K. 2012. Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus. Virus Genes 44: 349–355. DOI: http://dx.doi.org/10.1007/s112....
4.
Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
5.
Jeżewska M. 2001. First report of Wheat dwarf virus occurring in Poland. Phytopathologica Polonica 21: 93–100.
6.
Jeżewska M., Cajza M., Buchowska-Ruszkowska M. 2010. Monitoring i diagnostyka molekularna wirusów zbóż. [Monitoring and molecular diagnostics of cereal viruses]. p. 157–180. In: „Ograniczanie strat w plonach roślin uprawnych z zachowaniem bezpieczeństwa żywności”. [“Reducing losses in crop yields with food safety”] (D. Sosnowska, ed.). Instytut Ochrony Roślin – PIB, Poznań.
7.
Klejdysz T., Wałkowski W. 2008. Dynamika liczebności wybranych populacji piewików (Hemiptera: Auchenorrhyncha) występujących na jęczmieniu jarym w polowej stacji doświadczalnej Instytutu Ochrony Roślin w Winnej Górze na tle zmian klimatycznych. [Frequency dynamics of the selected populations of Auchenorrhyncha (Hemiptera) occurring on spring barley at Winna Góra on background of climatic change]. Progress in Plant Protection/Postępy w Ochronie Roślin 48 (3): 864–872.
8.
Klejdysz T. 2013. Piewiki (Hemiptera: Cicadomorpha & Fulgo-romorpha) jako składnik szkodliwej entomofauny ważniejszych upraw w Polsce. [Leafhoppers and planthoppers (Hemiptera: Cicadomorpha & Fulgoromorpha) as a component of the harmful entomofauna of most important crops in Poland]. Ph.D. thesis, Institute of Plant Protection – PIB, Poznań, Poland, 250 pp.
9.
Kundu J.K., Gadiou S., Červená G. 2009. Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic. Virus Genes 38: 468–474. DOI: 10.1007/s11262-009-0352-3.
10.
Lindblad M., Sandgren M., Sigvald R. 1999. Epidemiology and control of wheat dwarf. In: Proccedings of VIIth International Plant Virus Epidemiology Symposium. Aguadulce (Almeria), Spain, 11–16 April 1999, 114 pp.
11.
Lindsten K., Vacke J. 1991. A possible barley adapted strain of Wheat dwarf virus (WDV). Acta Phytopathologica et Entomologica Hungarica 26: 175–180.
12.
Manurung B., Witsack W., Mehner S., Grüntzig M., Fuchs E. 2004. The epidemiology of Wheat dwarf virus in relation to occurrence of the leafhopper Psammotettix alienus in Middle-Germany. Virus Research 100 (1): 109–113. DOI: https://doi.org/10.1016/j.viru....
13.
Mehner S., Manurung B., Grüntzig M., Habekuß A., Witsack W., Fuchs E. 2003. Investigations into the ecology of the Wheat dwarf virus (WDV) in Saxony-Anhalt, Germany. Journal of Plant Disease Protection 110 (4): 313–323.
14.
Muhire B., Martin D.P., Brown J.K., Navas-Castillo J., Moriones E., Zerbini F.M., Rivera-Bustamante R., Malathi V.G., Briddon R.W., Varsani A. 2013. A genome-wide pairwise-identity-based proposal for the classification of the viruses in the genus Mastrevirus (family Geminiviridae). Archives of Virology 158 (6): 1411–1424. DOI: https://doi.org/10.1007/s00705....
15.
Nowacka W. 1982. Skoczki (Homoptera, Auchenorrhyncha) występujące na uprawach zbóż i traw nasiennych na terenie Polski. [Leafhoppers (Homoptera, Auchenorryncha) occurring on cereals and seed grass crops in Poland]. Roczniki Akademii Rolniczej w Poznaniu, Rozprawy Naukowe, Zeszyt 122: 81 pp.
16.
Rabenstein F., Sukhacheva E., Habekuß A., Schubert J. 2005. Differentiation of Wheat dwarf virus isolates from wheat, triticale and barley by means of a monoclonal antibody p. 60. In: Proceedings of the X Conference on Viral Diseases of Gramineae in Europe, Louvain-la-Neuve, Belgium, 12–14 September 2005.
17.
Rosen S., Skaletski H.J. 2000. Primer3 on the WWW for general use and for biologist programmers. p. 365–386. In: “Bioinformatics Methods and Protocols: Methods in Molecular Biology” (S. Krawetz, S. Misenes, eds.). Totova/New Jersey, Humana Press. DOI: https://doi.org/10.1385/1-5925...: 365.
18.
Schubert J., Habekuß A., Kazmaier K., Jeske H., 2007. Surveying cereal-infecting geminiviruses in Germany – Diagnostic and direct sequencing using rolling circle amplification. Virus Research 127 (1): 61–70. DOI: https://doi.org/10.1016/j.viru....
19.
Schubert J., Habekuß A., Wu B., Thieme T., Wang X. 2014. Analysis of complete genomes of isolates of the Wheat dwarf virus from new geographical locations and descriptions of their defective forms. Virus Genes 48 (1): 133–139. DOI: https://doi.org/10.1007/s11262....
20.
Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W In: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choise. Nucleic Acids Research 22 (22): 4673–4680.
21.
Vacke J. 1961. Wheat dwarf virus disease. Biologia Plantarum (Praha) 3 (3): 228–233.
22.
Vacke J. 1962. Some new findings on wheat dwarf virus. p. 331–334. In: Plant Virology, Proceedings of the 5th Conference of the Czechoslovak Plant Virologists, Publishing House of the Czechoslovak Academy of Sciences, Prague, Czechoslovakia.
23.
Vacke J., Kvarnheden A., Lindbland M., Lindsten K. 2004. Wheat dwarf. p. 590–593. In: “Viruses and Virus Diseases of Poaceae (Gramineae)” (H. Lapierre, P.A. Signoret, eds). INRA, Paris, 857 pp.
24.
Wu B., Shang X., Schubert J., Habekuß A., Elena S.F., Wang X. 2015. Global-scale computational analysis of genomic sequences reveals the recombination pattern and coevolution dynamics of cereal-infecting geminiviruses. Scientific Reports 5: e 8153. DOI: https://doi.org/10.1038/srep08....
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