Detection of barley- and wheat-specific forms of Wheat Dwarf Virus in it's vector Psammotettix alienus by duplex PCR assay
Tomasz Klejdysz 2, A-C,E-F
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Department of Virology and Bacteriology, Institute of Plant Protection − National Research Institute, Władysława Węgorka 20, Poznań, Poland
Department of Entomology, Institute of Plant Protection − National Research Institute, Władysława Węgorka 20, Poznań, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2017-10-06
Acceptance date: 2017-12-18
Corresponding author
Katarzyna Trzmiel   

Department of Virology and Bacteriology, Institute of Plant Protection − National Research Institute, Władysława Węgorka 20, Poznań, Poland
Journal of Plant Protection Research 2018;58(1):54-57
Wheat dwarf virus (WDV) has been one of the most common viruses on cereal crops in Poland, in the last years. This ss DNA virus is transmitted by leafhopper, Psammotettix alienus (Dahlb.) in persistent manner. It induces yellowing and streaking of leaves, dwarfing or even death of infected plants. The presence of barley- and wheat- specific forms of WDV (WDV-B and WDV-W) and its vector was previously reported in the country, however the literature data did not include any information on the vectors infectivity in Poland. A duplex polymerase chain reaction (PCR) procedure was developed and optimized for simultaneous detection and differentiation of both WDV forms in its vector. Two sets of primers amplify 734 bp and 483 bp specific fragments for WDV-W and WDV-B, respectively. The results were verified by sequencing method. The studies were carried out on a set of insect samples collected in autumn from four different locations in Greater Poland. The results confirmed the presence of WDV-W in tested samples as well as suggested the possibility of two forms of the virus in the vector. Additional studies to determine virus-vector relationships should be undertaken.
The authors have declared that no conflict of interests exist.
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