First report of twig blight of ban tulsi (Croton bonplandianus Bail.) caused by Choanephora cucurbitarum (Berk. & Ravenel) Thaxt. in India
More details
Hide details
Department of Plant Pathology, Bidhan Chandra Krishi Viswa Vidyalaya, Mohanpur, Nadia, West Bengal 741252, India
Submission date: 2017-07-14
Acceptance date: 2017-10-07
Corresponding author
Subrata Dutta
Department of Plant Pathology, Bidhan Chandra Krishi Viswa Vidyalaya, Mohanpur, Nadia, West Bengal 741252, India
Journal of Plant Protection Research 2017;57(4):441–446
In August 2015, twig blight disease of ban tulsi (Croton bonplandianus Bail.) caused by Choanephora cucurbitarum (Berk. & Ravenel) Thaxt. was observed for the first time, in the Gangetic alluvial region of West Bengal, India. A severe disease incidence (40–50%) showed twig blight symptoms starting with shoot apical meristem (SAM), leaf, and blossom blight symptoms. Typical symptoms were characterized by over-projecting black pin head like emerging sporangiola which formed mycelial cushion on the infected surface. The present report describes the identification of the causal pathogen as C. cucurbitarum based on its morphology and the internal transcribed spacer of its ribosomal DNA (rDNA – ITS) sequences with 100% identity of NCBI-GenBank published Choanephora database. Optimum temperatures, 28–30°C, coupled with high relative humidity (80–90%) during the monsoon season enhances the disease’s progress. To the best of our knowledge this is the first report of twig blight of C. bonplandianus, caused by C. cucurbitarum, in India as well as globally.
The authors have declared that no conflict of interests exist.
Agrios G.N. 2005. Plant Pathology. 5th ed. Academic Press, San Diego, CA, USA: 434–435.
Boysen M., Borja M., del Moral C., Salazar O., Rubio V. 1996. Identification at strain level of Rhizoctonia solani AG 4 isolates by direct sequence of asymmetric PCR products of the ITS regions. Current Genetics 29 (2): 174–181. DOI: https://doi.org/10.1007/s00294....
Das S., Dutta S., Mandal B. 2017. First report of Choanephora cucurbitarum, causing leaf blight of hyacinth bean in India. Journal of Plant Pathology 99 (2): 533–543. DOI: http://dx.doi.org/10.4454/jpp.....
Doyle J.J., Doyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Physics Bulletin 19: 11–15.
Fransworth N.R. 1994. Ethnopharmacology and drug development. p. 42–51. In: “Ethnobotany and the Search for New Drugs” (D.J. Chadwick, J. Marsh, eds). Wiley, Chichester, New York, 90 pp.
Ghosh P., Mandal A., Golam Rasul M. 2013. A new bioactive ursane-type triterpenoid from Croton bonplandianum Bail. Journal of Chemical Sciences 125 (2): 359–364. DOI: https://doi.org/10.1007/s12039....
Kaul V. 1967. Distribution and dispersal of Croton sparsiflorus Morong. Journal of Indian Botanical Society 46: 154–159.
Kong J.M., Goh N.K., Chia L.S., Chia T.F. 2003. Recent advances in traditional plants drugs and orchids. Acta Pharmacologia Sinica 24: 7–21.
Kwon J.H., Hyeong J.J. 2005. Soft rot of eggplant (Solanum melongena) caused by Choanephora cucurbitarum in Korea. Mycobiology 33 (3): 163–165. DOI: https://doi.org/10.4489/MYCO.2....
Phillipson J.D. 1995. A matter of some sensitivity. Phytochemistry 38 (6): 1319–1343. DOI: https://doi.org/10.1016/0031-9....
Saroj A., Kumar A., Qamar N., Alam M., Singh H.N., Khaliq A. 2012. First report of wet rot of Withania somnifera caused by Choanephora cucurbitarum in India. Plant Disease 96 (2): 293. DOI: https://doi.org/10.1094/pdis-0....
Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. DOI: https://doi.org/10.1093/molbev....
Tapsell L.C. 2006. Health benefits of herbs and spices: the past, the present, the future. Medical Journal of Australia (4 Suppl.): 1–24.
Tiwary K.P., Choudhary R.N., Pandey G.D. 1981. A new alkaloid, 3-methoxy-4,6-dihydroxymorphinandien-7-one, and norsinoacutine have been isolated from extracts of Croton bonplandianum. Phytochemistry 20 (4): 863–864.
Walther G., Pawlowska J., Alastruey-Izquierdo A., Wrzosek M., Rodriguez-Tudela J.L., Dolatabadi S., Chakrabarti A., de Hoog G.S. 2013. DNA barcoding in mucorales: an inven-tory of biodiversity. Persoonia – Molecular Phylogeny and Evolution of Fungi 30 (1): 11–47. DOI: https://doi.org/10.3767/003158....
White T.J., Bruns T., Lee S., Taylor J. 1990. Amplification and direct sequencingof fungal ribosomal RNA genes for phylogenetics. p. 315–322. In: “PCR Protocols. a Guide to Methods and Applications” (M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White, eds). Academic Press, London, UK. DOI: https://doi.org/10.1016/b978-0....
WHO. 2001. Legal status of traditional medicines and complementary/alternative medicine: a worldwide review. World Health Organization, Geneva, 200 pp.